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Archives of Gynecology and Obstetrics

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01.11.2013 | Reproductive Medicine

Polymorphisms in MTHFR, MTHFD, and PAI-1 and recurrent miscarriage among North Indian women

verfasst von: Farah Parveen, Moni Tuteja, Suraksha Agrawal

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 5/2013

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Abstract

Purpose

The aim of this study was to investigate the association between MTHFR C677T, A1298C, MTHFD G1958A and plasminogen activator inhibitor type 1 (PAI-1) 4G/5G polymorphism among first trimester recurrent miscarriages.

Materials and methods

DNA was extracted from peripheral blood samples from 200 patients and 300 controls. Polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) and sequencing were used to identify the polymorphisms. We have analyzed the frequencies, odds ratio, Hardy–Weinberg equilibrium.

Results

MTHFR C677T, A1298C, and MTHFD G1958A variant alleles were found to be significantly more prevalent in patients than control. However, variant genotype of MTHFR C677T (OR = 2.54; 95 % CI = 1.23–5.24; p value = 0.014), 1298C (OR = 2.23; 95 % CI = 1.09–4.52; p value = 0.028), and MTHFD-1958 showed significant association with pregnancy loss (OR = 2.36; 95 % CI = 1.39–4.02; p value = 0.002). Both MTHFR 677 and MTHFD 1958 showed susceptible effect under recessive model of inheritance. PAI-1 mutations showed no significance.

Conclusion

We observed significant susceptible effects of MTHFR C677T, A1298C, and MTHFD G1958A among RM cases. Our data points toward the multifactorial nature of the recurrent miscarriage as relative contribution of variant genotype of MTHFR C677T is only twofold and further decreased to only onefold, and MTHFD-1958 lost its significance upon meta-analysis.

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Titel: Polymorphisms in MTHFR, MTHFD, and PAI-1 and recurrent miscarriage among North Indian women
verfasst von: Farah Parveen
Moni Tuteja
Suraksha Agrawal
Publikationsdatum: 01.11.2013
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 5/2013
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-013-2877-x

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Polymorphisms in MTHFR, MTHFD, and PAI-1 and recurrent miscarriage among North Indian women

Abstract

Purpose

Materials and methods

Results

Conclusion

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Abstract

Purpose

Materials and methods

Results

Conclusion

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