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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Population genetic structure of Plasmodium falciparum across a region of diverse endemicity in West Africa

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Victor A Mobegi, Kovana M Loua, Ambroise D Ahouidi, Judith Satoguina, Davis C Nwakanma, Alfred Amambua-Ngwa, David J Conway
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-223) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

VAM and DJC designed the experiments. VAM performed the experiments. VAM and DJC analysed the data. VAM, KML, ADA, JS, DCN, AA-N, and DJC provided materials and tools. VAM and DJC wrote the paper. All authors read and approved the final manuscript.

Abstract

Background

Malaria parasite population genetic structure varies among areas of differing endemicity, but this has not been systematically studied across Plasmodium falciparum populations in Africa where most infections occur.

Methods

Ten polymorphic P. falciparum microsatellite loci were genotyped in 268 infections from eight locations in four West African countries (Republic of Guinea, Guinea Bissau, The Gambia and Senegal), spanning a highly endemic forested region in the south to a low endemic Sahelian region in the north. Analysis was performed on proportions of mixed genotype infections, genotypic diversity among isolates, multilocus standardized index of association, and inter-population differentiation.

Results

Each location had similar levels of pairwise genotypic diversity among isolates, although there were many more mixed parasite genotype infections in the south. Apart from a few isolates that were virtually identical, the multilocus index of association was not significant in any population. Genetic differentiation between populations was low (most pairwise FST values < 0.03), and an overall test for isolation by distance was not significant.

Conclusions

Although proportions of mixed genotype infections varied with endemicity as expected, population genetic structure was similar across the diverse sites. Very substantial reduction in transmission would be needed to cause fragmented or epidemic sub-structure in this region.
Zusatzmaterial
Additional file 1: Table S1. Alleles scored in each of the 268 P. falciparum isolates genotyped at 10 microsatellite loci. Microsatellite alleles detected in isolates from the eight sampled populations, with highlighting showing predominant allele calls in the mixed genotypes. (DOCX 70 KB)
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Additional file 2: Table S2. Allele frequencies at 10 microsatellite loci in each of the eight population samples of Plasmodium falciparum. Data on frequency of each allele scored for each microsatellite locus in each of the eight populations sampled. (DOCX 46 KB)
12936_2012_2151_MOESM2_ESM.docx
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Literatur
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