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01.12.2018 | Research | Ausgabe 1/2018 Open Access

Immunity & Ageing 1/2018

Porphyromonas gingivalis, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice

Zeitschrift:
Immunity & Ageing > Ausgabe 1/2018
Autoren:
Ye Ding, Jingyi Ren, Hongqiang Yu, Weixian Yu, Yanmin Zhou
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12979-017-0110-7) contains supplementary material, which is available to authorized users.

Abstract

Background

A possible relationship between periodontitis and Alzheimer’s disease (AD) has been reported. However, there is limited information on the association between the Porphyromonas gingivalis (P. gingivalis) periodontal infection and the pathological features of AD. The hypothesis that P. gingivalis periodontal infection may cause cognitive impairment via age-dependent neuroinflammation was tested.

Results

Thirty 4-week-old (young) female C57BL/6 J mice were randomly divided into two groups, the control group and the experimental group. Thirty 12-month-old (middle-aged) were grouped as above. The mouth of the mice in the experimental group was infected with P. gingivalis. Morris water maze(MWM) was performed to assess the learning and memory ability of mice after 6 weeks. Moreover, the expression levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the mice brain tissues were determined by Quantitative real-time polymerase chain reaction (qRT-PCR), Enzyme Linked Immunosorbent Assay(ELISA) and immunohistochemistry. Our results showed that the learning and memory abilities of the middle-aged P. gingivalis infected mice were impaired. Moreover, the expression levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the brain tissues of the middle-aged P. gingivalis infected mice were increased.

Conclusions

These results suggest that P. gingivalis periodontal infection may cause cognitive impairment via the release of the pro-inflammatory cytokines TNF-α, IL-6, and IL-1β in the brain tissues of middle-aged mice.
Zusatzmaterial
Additional file 1: Table 1. Escape latency time(s) assessed by the MWM test in young and middle-aged mice. Table 2. The number of platform crossings assessed by the MWM test. Table 3. Analysis of the TNF-α, IL-6, IL-1β mRNA expression levels in mice brain tissues by qRT-PCR. Table 4. The protein expression levels of TNF-α, IL-6, IL-1β in brain samples, measured by ELISA. Table 5. The mean number of the TNF-α, IL-6, IL-1β positive cells per section. (XLSX 14 kb)
12979_2017_110_MOESM1_ESM.xlsx
Literatur
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