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05.11.2016 | Original Article

Position 22 of the V3 loop is associated with HIV infectivity

verfasst von: Xue-Mei Wei, Hua-Feng Xu, Xue-Di Cheng, Nan Bu, Hai-Zhou Zhou

Erschienen in: Archives of Virology | Ausgabe 3/2017

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Abstract

Human immunodeficiency virus subtype 1B (HIV-1B) binds to the CD4 receptor and co-receptor CCR5 or CXCR4 to enter T lymphocytes. The amino acid sequence of the HIV envelope glycoprotein V3 region determines the co-receptor tropism, thereby influencing the infectivity of the virus. Our research group previously found that the amino acid at position 22 of the V3 region may affect the infectivity of the virus, and in this study, we tested this hypothesis. We constructed pseudoviruses by changing the amino acids at position 22 of the V3 region in CCR5-tropic and CXCR4-tropic viruses and tested their infectivity. When the amino acid at V3 position 22 was altered in the CCR5- and CXCR4-tropic viruses, their ability to infect cells decreased to 20.6% and 17.14%, respectively. Therefore, we propose that residue 22 in the V3 region of subtype HIV-1B significantly influences the infectivity of the virus.

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Titel: Position 22 of the V3 loop is associated with HIV infectivity
verfasst von: Xue-Mei Wei
Hua-Feng Xu
Xue-Di Cheng
Nan Bu
Hai-Zhou Zhou
Publikationsdatum: 05.11.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 3/2017
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-3138-7

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