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07.05.2018 | Original Article

Potential forensic biogeographic application of diatom colony consistency analysis employing pyrosequencing profiles of the 18S rDNA V7 region

verfasst von: Yuancun Zhao, Xiaogang Chen, Yiwen Yang, Xiaohong Zhao, Shu Zhang, Zehua Gao, Ting Fang, Yufang Wang, Ji Zhang

Erschienen in: International Journal of Legal Medicine | Ausgabe 6/2018

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Abstract

Diatom examination has always been used for the diagnosis of drowning in forensic practice. However, traditional examination of the microscopic features of diatom frustules is time-consuming and requires taxonomic expertise. In this study, we demonstrate a potential DNA-based method of inferring suspected drowning site using pyrosequencing (PSQ) of the V7 region of 18S ribosome DNA (18S rDNA) as a diatom DNA barcode. By employing a sparse representation-based AdvISER-M-PYRO algorithm, the original PSQ signals of diatom DNA mixtures were deciphered to determine the corresponding taxa of the composite diatoms. Additionally, we evaluated the possibility of correlating water samples to collection sites by analyzing the PSQ signal profiles of diatom mixtures contained in the water samples via multidimensional scaling. The results suggest that diatomaceous PSQ profile analysis could be used as a cost-effective method to deduce the geographical origin of an environmental bio-sample.

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Titel: Potential forensic biogeographic application of diatom colony consistency analysis employing pyrosequencing profiles of the 18S rDNA V7 region
verfasst von: Yuancun Zhao
Xiaogang Chen
Yiwen Yang
Xiaohong Zhao
Shu Zhang
Zehua Gao
Ting Fang
Yufang Wang
Ji Zhang
Publikationsdatum: 07.05.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: International Journal of Legal Medicine / Ausgabe 6/2018
Print ISSN: 0937-9827
Elektronische ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-018-1849-x

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