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Erschienen in: Japanese Journal of Ophthalmology 5/2017

08.06.2017 | Clinical Investigation

Pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty

verfasst von: Takenori Inomata, Koichi Ono, Tsuyoshi Matsuba, Tina Shiang, Antonio Di Zazzo, Satoru Nakatani, Masahiro Yamaguchi, Nobuyuki Ebihara, Akira Murakami

Erschienen in: Japanese Journal of Ophthalmology | Ausgabe 5/2017

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Abstract

Purpose

The aims of this study were to investigate the incidence of positive donor tissue cultures before transfer to preservation medium (Optisol™-GS) for penetrating keratoplasty, to verify the efficacy of antibiotics contained in Optisol™-GS by examining the drug susceptibility and to assess the relationship between the results of our microbial assessments as well as donor factors and the incidence of contamination.

Methods

We conducted a retrospective, cross-sectional study using Juntendo Eye Bank records for all corneal transplantations. Two hundred donor conjunctiva harvestings and storage medium (EP-II®) cultures were performed between July 2008 and June 2011. We analyzed the associations between donor factors (age, gender, history of cataract surgery, death-to-preservation interval, cause of death) and contamination rates using multivariate analysis by the generalized estimating equation model.

Results

We obtained positive bacterial cultures from 154 of the 200 eyes (77.0%). The isolated bacteria were indigenous, such as coagulase-negative Staphylococci, Corynebacterium sp., and methicillin-resistant Staphylococcus aureus (MRSA). There was significant resistance to levofloxacin (18 eyes, 9.0%) and gentamicin (12 eyes, 6.0%), and no vancomycin-resistant bacteria were detected. The donor factors did not correlate with the prevalence of bacterial contamination in our criteria.

Conclusions

Pre-banking microbial assessment allows for microbial detection, bacterial susceptibility and resistance testing. This is useful for developing preservation mediums containing effective spectrum antibiotic agents for high quality control of corneal banking.
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Metadaten
Titel
Pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty
verfasst von
Takenori Inomata
Koichi Ono
Tsuyoshi Matsuba
Tina Shiang
Antonio Di Zazzo
Satoru Nakatani
Masahiro Yamaguchi
Nobuyuki Ebihara
Akira Murakami
Publikationsdatum
08.06.2017
Verlag
Springer Japan
Erschienen in
Japanese Journal of Ophthalmology / Ausgabe 5/2017
Print ISSN: 0021-5155
Elektronische ISSN: 1613-2246
DOI
https://doi.org/10.1007/s10384-017-0521-1

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