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01.12.2018 | Research article | Ausgabe 1/2018 Open Access

BMC Cancer 1/2018

Preoperative serum immunoglobulin G and A antibodies to Porphyromonas gingivalis are potential serum biomarkers for the diagnosis and prognosis of esophageal squamous cell carcinoma

Zeitschrift:
BMC Cancer > Ausgabe 1/2018
Autoren:
She-Gan Gao, Jun-Qiang Yang, Zhi-Kun Ma, Xiang Yuan, Chen Zhao, Guang-Chao Wang, Hua Wei, Xiao-Shan Feng, Yi-Jun Qi
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12885-017-3905-1) contains supplementary material, which is available to authorized users.

Abstract

Background

The key-stone-pathogen, Porphyromonas gingivalis associates not only with periodontal diseases but with a variety of other chronic diseases such as cancer. We previously reported an association between the presence of Porphyromonas gingivalis in esophageal squamous cell carcinoma (ESCC) and its progression. We now report the diagnostic and prognostic potential of serum immunoglobulin G and A antibodies (IgG/A) against Porphyromonas gingivalis for ESCC.

Methods

An enzyme-linked immunosorbent assay (ELISA) was used to determine the serum levels of Porphyromonas gingivalis IgG and IgA in 96 cases with ESCC, 50 cases with esophagitis and 80 healthy controls.

Results

The median serum levels of IgG and IgA for P. gingivalis were significantly higher in ESCC patients than non-ESCC controls. P. gingivalis IgG and IgA in serum demonstrated sensitivities/specificities of 29.17%/96.90% and 52.10%/70.81%, respectively, and combination of IgG and IgA produced a sensitivity/specificity of 68.75%/68.46%. The diagnostic performance of serum P. gingivalis IgA for early ESCC was superior to that of IgG (54.54% vs. 20.45%). Furthermore, high serum levels of P. gingivalis IgG or IgA were associated with worse prognosis of ESCC patients, in particular for patients with stage 0-IIor negative lymphnode metastasis, and ESCC patients with high levels of both IgG and IgA had the worst prognosis. Multivariate analysis revealed that lymph node status, IgG and IgA were independent prognostic factors.

Conclusions

The IgG and IgA for P. gingivalis are potential serum biomarkers for ESCC and combination of IgG and IgA improves the diagnostic and prognostic performance. Furthermore, serum P. gingivalis IgG and IgA can detect early stage ESCC.
Zusatzmaterial
Additional file 1: Figure S1. Kaplan-Meier survival curves of ESCC patients with regards to clinical stage. A The 3-year OS rates in ESCC patients with TNMI-II (n = 51) and patients with TNM III-IV (n = 27) were 59.95% and 33.26%, respectively (P = 0.069). B The 3-year OS rates in ESCC patients with IgG < 138.23 EU (n = 59) and IgG > 138.23 EU (n = 19) were 77.59% and 37.65%, respectively, in early clinical stage (P = 0.055). B The 3-year OS rates in ESCC patients with IgG < 138.23 EU (n = 59) and IgG > 138.23 EU (n = 19) were 44.63% and 20.89%, respectively, in late clinical stage (P = 0.055). D The 3-year OS rates in ESCC patients with IgA < 56.56 EU (n = 62) and IgA > 56.56 EU (n = 16) were 68.95% and 23.34%, respectively, in early clinical stage (P = 0.003). D The 3-year OS rates in ESCC patients with IgA < 56.56 EU (n = 62) and IgA > 56.56 EU (n = 16) were 41.45% and 0, respectively, in late clinical stage (P = 0.48). (DOC 334 kb)
12885_2017_3905_MOESM1_ESM.doc
Additional file 2: Figure S2. Kaplan-Meier survival curves of ESCC patients with regards to lymph node stage. A The 3-year OS rates in ESCC patients without lymph node metastasis (n = 44) and patients with lymph node metastasis (n = 34) were 63.87% and 27.85%, respectively (P = 0.018). B The 3-year OS rates in ESCC patients with IgG < 138.23 EU (n = 59) and IgG > 138.23 EU (n = 19) were 87.19% and 37.64%, respectively, in negative lymph node metastasis (P = 0.014). C The 3-year OS rates in ESCC patients with IgG < 138.23 EU (n = 59) and IgG > 138.23 EU (n = 19) were 29.43% and 20.80%, respectively, in lymph node metastasis (P = 0.293). D The 3-year OS rates in ESCC patients with IgA < 56.56 EU (n = 62) and IgA > 56.56 EU (n = 16) were 72.91% and 25.96%, respectively, in negative lymph node metastasis (P = 0.011). E The 3-year OS rates in ESCC patients with IgA < 56.56 EU (n = 62) and IgA > 56.56 EU (n = 16) were 34.52% and 0, respectively, in lymph node metastasis (P = 0.092). (DOC 355 kb)
12885_2017_3905_MOESM2_ESM.doc
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