The online version of this article (https://doi.org/10.1186/s12879-017-2911-x) contains supplementary material, which is available to authorized users.
Urinary tract infection (UTI) remains to be one of the most common infectious diseases diagnosed in developing countries. And a widespread use of antibiotics against uropathogens has led to the emergence of antibiotic resistant species. A laboratory based cross-sectional survey was conducted in Shashemene referral hospital to determine the prevalence and antibiotic susceptibility of uropathogens.
We have collected 384 clean catch mid-stream urine samples from all suspected UTI outpatients using sterile screw capped container. The urine samples were cultured and processed for subsequent uropathogens isolation. The isolated pure cultures were grown on BiOLOG Universal Growth agar (BUG) and identified using GEN III OmniLog® Plus ID System identification protocols. The identified species were then exposed to selected antibiotics to test for their susceptibility.
The overall prevalence of urinary tract infection in the area was 90.1%. Most frequently isolated uropathogen in our study was Escherichia coli (39.3%). While, Staphylococcus species (20.2%), Leuconostoc species (11.4%), Raoultella terrigena/Klebsiella spp./ (8.4%), Salmonella typhimurium (6.3%), Dermacoccus nishinomiyaensis (6.3%), Citerobacter freundii (5.2%) and Issatchenkia orientalis/Candida krusei/ (2.7%) were the other isolates. We find that the relationship between uropathogens and some of UTI risk factors was statistically significant (P < 0.05). Gentamicin was the most effective drug against most of the isolates followed by chloramphenicol and nitrofurantoin. In contrast, amoxicillin, vancomycin and cephalexin were the antibiotics to which most of the isolates developed resistance.
Urinary tract infection was highly prevalent in the study area and all uropathogens isolated developed a resistance against mostly used antibiotics.
Additional file 1: Questionnaire. (DOCX 102 kb)12879_2017_2911_MOESM1_ESM.docx
Additional file 2: Figures S1. Different pictures of laboratory processes. The picture indicates the detail of processes followed in the study including outpatients interview, bacterial inoculation and incubation and further analyses. (DOC 1081 kb)12879_2017_2911_MOESM2_ESM.doc
Additional file 3: Tables S1. Univariate and Multivariate logistic regression of risk factors pertinent to females for the prevalence of UTI. Univariate and multivariate logistic regression were used to correlate risk factors and prevalence of UTI. Except diaphragm use, the rest risk factors have significant association with prevalence of UTI in females. (XLSX 44 kb)12879_2017_2911_MOESM3_ESM.xlsx
Additional file 4: Table S2. The proportion of sensitive, intermediate and resistant bacterial isolates to eleven different antibiotics. The table indicates the sensitivity of isolates to various antibiotics used in the study. (XLSX 41 kb)12879_2017_2911_MOESM4_ESM.xlsx
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- Prevalence and antibiotic susceptibility of Uropathogens from cases of urinary tract infections (UTI) in Shashemene referral hospital, Ethiopia
Wubalem Desta Seifu
Alemayehu Desalegn Gebissa
- BioMed Central
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