Background
Methods
Study site and population
Ethical consideration
Anthropometric measurements
Fecal sample collection, processing, and analysis
Collection of information from children and their families
Statistical analysis
Results
Prevalence of Giardia and Cryptosporidium
Status (n)a
| Percentage (95% CI)d
|
---|---|
Uninfected (70) | 40 (33–48) |
Infectionb (103) | 60 (52–67) |
Polyparasitismc(50) | 29 (22–36) |
Pathogenics | |
Cryptosporidium spp. (47) | 27 (20–33) |
Giardia intestinalis (40) | 23 (17–29) |
Entamoeba histolytica/dispar/moshkovskii
e (4) | 2 (0.5–4.5) |
Commensal | |
Endolimax nana (57) | 33 (26–40) |
Entamoeba coli (29) | 17 (11–23) |
Iodamoeba bütschlii (1) | 0.6 (0.02–1.7) |
Infection and nutritional status
Variable | Free of pathogenic species | *G. intestinalis infected |
p valuee
|
---|---|---|---|
n = 93 |
n = 40 | ||
Age (years) | 8.5 (±2.9) | 8.7 (±2.9) | 0.716c
|
Weight (kg)a
| 21.6 (18.2–23.9) | 25.6 (20.8–31.6) | 0.379b
|
Height (cm) | 125.2 (±24.3) | 123.5 (±19.2) | 0.667c
|
W/A (Z score)d
| 0.31 (±1.1) (n = 65) | −0.14 (±1.1) (n = 25) | 0.089c
|
H/A (Z score) | −0.11 (±1.6) | −1.05 (±1.0) | 0.001c
|
BMI/A (Z score) | 0.67 (±1.3)b
| 0.06 (±1.5) | 0.028c
|
Free of pathogenic species | **Cryptosporidium spp. infected | ||
n = 93 |
n = 47 | ||
Age (years) | 8.5 (±2.9) | 9.2 (±2.8) | 0.170c
|
Weight (kg)a
| 21.6 (18.2–23.9) | 29.1 (23.2–36.7) | 0.201b
|
Height (cm) | 125.2 (±24.3) | 124.3 (13.1) | 0.776c
|
W/A (Z score)d
| 0.31 (±1.1) (n = 65) | −0.09 (±1.1) (n = 35) | 0.087c
|
H/A (Z score) | −0.11 (±1.6) | −0.83 (±1.1) | 0.002c
|
BMI/A (Z score) | 0.67 (±1.32)b
| 0.13 (±1.4) | 0.030c
|
Distribution of risk factors of the families of participating children
Characteristic | Number of interviews | Categories | Percentage (n) |
---|---|---|---|
Civil status | |||
Mother | 173 | Married | 32 (56) |
Unmarried (free union, widow, single) | 68 (117) | ||
Father | 45a
| Married | 30 (14) |
Unmarried (free union) | 70 (31) | ||
Economic activity | |||
Mother | 173 | Yes (sales, teacher, babysitter, adult patient care, cleaning of homes, maid, laundry, merchant, employee, retired) | 43 (74) |
No (housewife, student) | 57 | ||
Father | 45 | Yes (sowing, mechanic, laborer, sales, miner, merchant, painter, teacher, blacksmith, carpenter, welder) | 88 (40) |
No (student) | 12 | ||
Education | |||
Mother | 173 | Completed secondary | 25 (40) |
Uncompleted secondary | 75 | ||
Father | 45 | Completed secondary | 25 (11) |
Uncompleted secondary | 75 | ||
Family Income | 173 | ||
˃ 4.86 USD | 21 (36) | ||
≤4.86 USD (≤1 minimum wage/day) | 79 | ||
Crowding | 173 | ||
<3 persons per room | 76 (131) | ||
>3 persons per room | 24 | ||
Household characteristics | 173 | ||
Walls material | Cement, brick, block, adobe | 90 (156) | |
Other material (cardboard) | 10 | ||
Floor material | Laminate, mosaic, cement | 90 (156) | |
Ground | 10 | ||
Roof material | Cement, wood and metal laminate | 90 (156) | |
Only metal laminate | 10 | ||
Drinking Water | 173 | ||
Treated water (boiled or with iodine and/or chlorine solution or commercial purified water) | 41 (71) | ||
Direct tap water (only with chlorine 5 ppm). | 59 | ||
Symptomatology | 173 | ||
Abdominal pain | |||
Yes | 64 (111) | ||
No | 36 | ||
Headache | |||
Yes | 46 (80) | ||
No | 54 | ||
Domestic animals | 173 | ||
No | 57 (99) | ||
Yes (chickens, dogs, cats) | 43 |
Factors associated with G. intestinalis and Cryptosporidium spp.
Giardia
|
Cryptosporidium
| |||||||
---|---|---|---|---|---|---|---|---|
Variable | n |
Giardia infecteda
| OR (95%CI) |
p* | n |
Cryptosporidium spp. infectedb
| OR (95%CI) |
p* |
Drinking water | 40 | 47 | ||||||
Treated water | 9 | 1.0 | 12 | 1.0 | ||||
Tap water | 31 | 3.9 (1.7–8.9) | 0.014 | 35 | 3.9 (1.64–9.10) | 0.002 | ||
Keep domestic animals | 40 | 47 | ||||||
No | 23 | 1.0 | 20 | 1.0 | ||||
Yes | 17 | 2.8 (1.30–5.88) | 0.008 | 27 | 3.5 (1.55–7.56) | 0.002 | ||
Symptoms | 40 | 47 | ||||||
No Abdominal pain | 23 | 1.0 | 16 | 1.0 | ||||
Abdominal pain | 17 | 3.2 (1.32–7.81) | 0.010 | 31 | 1.54 (0.69–3.45) | 0.290 | ||
No Headache | 23 | 1.0 | 27 | 1.0 | ||||
Headache | 17 | 2.9 (1.35–6.02) | 0.006 | 20 | 0.74 (0.35–1.51) | 0.390 | ||
Season for sample collection | 40 | 42 | ||||||
Autumn | 8 | 1.0 | 16 | 1.0 | ||||
Spring | 32 | 3.53 (1.45–8.56) | 0.005 | 26 | 1.1 (0.50–2.25) | 0.902 |
Giardia infecteda (n = 40) | OR | (95%CI) | *p
|
---|---|---|---|
Abdominal pain | 4.00 | 1.11–13.02 | 0.030 |
Sexc
| 1.81 | 0.67–4.90 | 0.237 |
Agec
| 0.84 | 0.65–1.05 | 0.178 |
W/A (Z score)c
| 2.81 | 0.98–8.09 | 0.054 |
H/A (Z score)c
| 0.30 | 0.15–0.64 | 0.002 |
BMI/A (Z score)c
| 0.13 | 0.18–0.92 | 0.032 |
Cryptosporidium spp. infectedb (n = 47) | OR | (95%CI) | *p
|
Tap water | 5.00 | 1.41–17.20 | 0.012 |
Sexc
| 0.47 | 0.15–1.40 | 0.190 |
Agec
| 1.56 | 1.11–2.18 | 0.010 |
W/A (Z score)c
| 3.88 | 1.23–12.20 | 0.020 |
H/A (Z score)c
| 0.39 | 0.20–0.77 | 0.007 |
BMI/A (Z score)c
| 0.35 | 0.14–0.85 | 0.210 |