Background
Methods
Study population and include criteria
Cervical sample collection
HPV DNA extraction and genotyping
Thin prep liquid-based cytology test
Histology diagnosis
Statistical analysis
Results
Prevalence of HPV infection in HEC and OGOC group
Age Group (Year) | Total case | HPV positive cases | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
HEC | OGOC | Total | HEC | OGOC | Total | |||||||
n | %a | n | %a | n | %a | n | %b | n | %b | n | %b | |
≤20 | 2 | 0.02 | 149 | 2.74 | 151 | 1.06 | 1 | 0.01 | 86 | 1.58 | 87 | 0.61 |
21–30 | 902 | 10.31 | 2111 | 38.85 | 3013 | 21.24 | 149 | 1.70 | 801 | 14.74 | 950 | 6.70 |
31–40 | 2722 | 31.11 | 1368 | 25.17 | 4090 | 28.83 | 404 | 4.62 | 462 | 8.50 | 866 | 6.11 |
41–50 | 3552 | 40.59 | 1201 | 22.10 | 4753 | 33.51 | 513 | 5.86 | 397 | 7.31 | 910 | 6.42 |
51–60 | 1262 | 14.42 | 457 | 8.41 | 1719 | 12.12 | 220 | 2.51 | 215 | 3.96 | 435 | 3.07 |
61–70 | 274 | 3.13 | 129 | 2.37 | 403 | 2.84 | 47 | 0.54 | 75 | 1.38 | 122 | 0.86 |
≥71 | 37 | 0.42 | 19 | 0.35 | 56 | 0.39 | 4 | 0.05 | 8 | 0.15 | 12 | 0.08 |
Total | 8751 | 100.00 | 5434 | 100.00 | 14,185 | 100.00 | 1338 | 15.29 | 2044 | 37.62 | 3382 | 23.84 |
Prevalence of HPV grouped by age in study population
Prevalence of HPV single and multiple HPV genotypes infection
Prevalence of HPV infection | Total HPV+ | HEC HPV+ | OGOC HPV+ | |||
---|---|---|---|---|---|---|
n | %a | n | %a | n | %a | |
Single Infection | 2099 | 62.06 | 995 | 74.36 | 1104 | 54.01 |
Mutlple Infection | 1283 | 37.94 | 343 | 25.64 | 940 | 45.99 |
Double Infection | 903 | 26.70 | 267 | 19.96 | 636 | 31.12 |
Triple Infection | 266 | 7.87 | 64 | 4.78 | 202 | 9.88 |
Quadruple Infection | 76 | 2.25 | 11 | 0.82 | 65 | 3.18 |
Quintet Infection | 22 | 0.65 | 0 | 0.00 | 22 | 1.08 |
Sextuple Infection | 10 | 0.30 | 1 | 0.07 | 9 | 0.44 |
Septuple Infection | 6 | 0.18 | 0 | 0.00 | 6 | 0.29 |
Total Infection | 3382 | 100.00 | 1338 | 100.00 | 2044 | 100.00 |
Prevalence of HPV genotypes in HPV-positive population
Total HPV+ | HEC HPV+ | OGOC HPV+ | pa | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
S%c (n = 2478) | M%d (n = 904) | T%e (n = 3382) | CI (95%) (n = 3382) | S%c (n = 1052) | M%d (n = 267) | T%e (n = 1319) | CI (95%) (n = 1319) | S%c (n = 1426) | M%d (n = 637) | T%e (n = 2063) | CI (95%) (n = 2063) | ||
High Risk | |||||||||||||
16 | 8.10 | 5.50 | 13.60 | 12.45–14.76 | 5.98 | 3.44 | 9.42 | 7.85–10.98 | 9.49 | 6.85 | 16.34 | 14.74–17.94 | < 0.001b |
18 | 2.28 | 2.69 | 4.97 | 4.24–5.70 | 2.24 | 1.79 | 4.04 | 2.98–5.09 | 2.30 | 3.28 | 5.58 | 4.58–6.57 | 0.044b |
31 | 1.77 | 1.77 | 3.55 | 2.92–4.17 | 1.79 | 1.27 | 3.06 | 2.14–3.99 | 1.76 | 2.10 | 3.86 | 3.03–4.70 | 0.218 |
33 | 2.75 | 2.28 | 5.03 | 4.29–5.76 | 3.21 | 1.42 | 4.63 | 3.51–5.76 | 2.45 | 2.84 | 5.28 | 4.31–6.25 | 0.398 |
35 | 0.50 | 0.59 | 1.09 | 0.74–1.44 | 0.75 | 0.45 | 1.20 | 0.61–1.78 | 0.34 | 0.68 | 1.03 | 0.59–1.46 | 0.645 |
39 | 4.82 | 4.46 | 9.28 | 8.31–10.26 | 5.53 | 3.44 | 8.97 | 7.44–10.50 | 4.35 | 5.14 | 9.49 | 8.22–10.76 | 0.609 |
45 | 0.33 | 0.68 | 1.01 | 0.67–1.34 | 0.45 | 0.52 | 0.97 | 0.45–1.50 | 0.24 | 0.78 | 1.03 | 0.59–1.46 | 0.874 |
51 | 3.78 | 4.70 | 8.52 | 7.57–9.46 | 4.71 | 3.36 | 8.07 | 6.61–9.53 | 3.18 | 5.58 | 8.81 | 7.58–10.03 | 0.454 |
52 | 12.89 | 8.16 | 21.05 | 19.68–22.43 | 14.13 | 6.80 | 20.93 | 18.75–23.11 | 12.08 | 9.05 | 21.14 | 19.37–22.90 | 0.884 |
53 | 6.80 | 5.32 | 12.12 | 11.02–13.22 | 9.49 | 4.26 | 13.75 | 11.91–15.60 | 5.04 | 6.02 | 11.06 | 9.70–12.42 | 0.019b |
56 | 1.21 | 1.86 | 3.08 | 2.49–3.66 | 1.12 | 1.27 | 2.39 | 1.57–3.21 | 1.27 | 2.25 | 3.52 | 2.72–4.32 | 0.063 |
58 | 9.05 | 6.09 | 15.14 | 13.93–16.35 | 9.42 | 5.01 | 14.42 | 12.54–16.31 | 8.81 | 6.80 | 15.61 | 14.03–17.18 | 0.348 |
59 | 1.12 | 1.27 | 2.40 | 1.88–2.91 | 0.90 | 0.97 | 1.87 | 1.14–2.59 | 1.27 | 1.47 | 2.74 | 2.03–3.45 | 0.105 |
66 | 1.98 | 2.25 | 4.23 | 3.55–4.91 | 2.54 | 1.42 | 3.96 | 2.92–5.01 | 1.61 | 2.79 | 4.40 | 3.51–5.29 | 0.532 |
68 | 2.40 | 2.72 | 5.12 | 4.37–5.86 | 2.77 | 2.32 | 5.08 | 3.91–6.26 | 2.15 | 2.98 | 5.14 | 4.18–6.09 | 0.944 |
Low Risk | |||||||||||||
6 | 2.96 | 3.64 | 6.62 | 5.79–7.46 | 0.82 | 0.90 | 1.72 | 1.02–2.42 | 4.35 | 5.48 | 9.83 | 8.54–11.12 | < 0.001b |
11 | 2.40 | 2.87 | 5.26 | 4.51–6.02 | 1.05 | 0.67 | 1.72 | 1.02–2.42 | 3.28 | 4.26 | 7.58 | 6.44–8.73 | < 0.001b |
42 | 0.06 | 0.30 | 0.35 | 0.15–0.56 | 0.07 | 0.00 | 0.07 | −0.07 ~ 0.22 | 0.05 | 0.49 | 0.54 | 0.22–0.86 | 0.055 |
43 | 0.18 | 0.30 | 0.47 | 0.24–0.70 | 0.15 | 0.07 | 0.22 | −0.03-0.48 | 0.20 | 0.44 | 0.64 | 0.29–0.98 | 0.088 |
44 | 1.01 | 0.98 | 1.98 | 1.51–2.45 | 1.42 | 1.05 | 2.47 | 1.64–3.30 | 0.73 | 0.93 | 1.66 | 1.11–2.22 | 0.101 |
CP8304 | 6.89 | 6.21 | 13.10 | 11.96–14.24 | 10.09 | 5.23 | 15.32 | 13.39–17.25 | 4.79 | 6.85 | 11.64 | 10.25–13.03 | p = 0.002b |
Distribution of HPV genotypes according to cytology abnormalities
ASCUS | LSIL | HSIL | AGC | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
S%a (n = 77) | M%b (n = 48) | T%c (n = 125) | S%a (n = 22) | M%b (n = 15) | T%c (n = 37) | S%a (n = 1) | M%b (n = 0) | T%c (n = 1) | S%a (n = 4) | M%b (n = 4) | T%c (n = 8) | |
High Risk | 81.41% | 88.57% | 100% | 100% | ||||||||
16 | 12.80 | 9.60 | 22.40 | 8.11 | 10.81 | 18.92 | 0.00 | 0.00 | 0.00 | 12.50 | 0.00 | 12.50 |
18 | 0.00 | 2.40 | 2.40 | 5.41 | 8.11 | 13.51 | 0.00 | 0.00 | 0.00 | 0.00 | 12.50 | 12.50 |
31 | 1.60 | 0.00 | 1.60 | 2.70 | 5.41 | 8.11 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
33 | 4.00 | 5.60 | 9.60 | 2.70 | 5.41 | 8.11 | 0.00 | 0.00 | 0.00 | 12.50 | 12.50 | 25.00 |
35 | 0.80 | 0.00 | 0.80 | 0.00 | 5.41 | 5.41 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
39 | 2.40 | 4.80 | 7.20 | 2.70 | 16.22 | 18.92 | 0.00 | 0.00 | 0.00 | 0.00 | 12.50 | 12.50 |
45 | 0.00 | 0.80 | 0.80 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
51 | 0.80 | 6.40 | 7.20 | 2.70 | 0.00 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 37.50 | 37.50 |
52 | 14.40 | 15.20 | 29.60 | 2.70 | 13.51 | 16.22 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
53 | 1.60 | 6.40 | 8.00 | 5.41 | 8.11 | 13.51 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
56 | 2.40 | 4.80 | 7.20 | 2.70 | 8.11 | 10.81 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
58 | 8.80 | 12.80 | 21.60 | 8.11 | 10.81 | 18.92 | 100.00 | 0.00 | 100.00 | 25.00 | 25.00 | 50.00 |
59 | 0.80 | 0.80 | 1.60 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
66 | 0.00 | 6.40 | 6.40 | 13.51 | 8.11 | 21.62 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
68 | 1.60 | 1.60 | 3.20 | 0.00 | 5.41 | 5.41 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Low Risk | 18.59% | 11.43% | 0.00% | 0.00% | ||||||||
6 | 2.40 | 3.20 | 5.60 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
11 | 2.40 | 2.40 | 4.80 | 2.70 | 2.70 | 5.41 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
42 | 0.00 | 0.00 | 0.00 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
43 | 0.00 | 1.60 | 1.60 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
44 | 0.80 | 2.40 | 3.20 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
CP8304 | 4.00 | 10.40 | 14.40 | 0.00 | 13.51 | 13.51 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Distribution of HPV genotypes according to histological abnormalities
CIN I | CIN II | CIN III | CA | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
S%a (n = 21) | M%b (n = 16) | T%c (n = 37) | S%a (n = 13) | M%b (n = 4) | T%c (n = 17) | S%a (n = 2) | M%b (n = 0) | T%c (n = 2) | S%a (n = 2) | M%b (n = 1) | T%c (n = 3) | |
High Risk | 89.83% | 96.30% | 100% | 100% | ||||||||
16 | 10.81 | 10.81 | 21.62 | 52.94 | 17.65 | 70.59 | 50.00 | 0.00 | 50.00 | 25.00 | 0.00 | 25.00 |
18 | 0.00 | 5.41 | 5.41 | 5.88 | 0.00 | 5.88 | 0.00 | 0.00 | 0.00 | 0.00 | 25.00 | 25.00 |
31 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
33 | 5.41 | 0.00 | 5.41 | 11.76 | 11.76 | 23.53 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
35 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
39 | 2.70 | 8.11 | 10.81 | 0.00 | 11.76 | 11.76 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
45 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
51 | 2.70 | 2.70 | 5.41 | 0.00 | 11.76 | 11.76 | 0.00 | 0.00 | 0.00 | 0.00 | 25.00 | 25.00 |
52 | 13.51 | 13.51 | 27.03 | 5.88 | 17.65 | 23.53 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
53 | 0.00 | 8.11 | 8.11 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
56 | 2.70 | 8.11 | 10.81 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
58 | 18.92 | 16.22 | 35.14 | 0.00 | 5.88 | 5.88 | 50.00 | 0.00 | 50.00 | 25.00 | 0.00 | 25.00 |
59 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
66 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
68 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
Low Risk | 10.17% | 3.70% | 0.00% | 0.00% | ||||||||
6 | 0.00 | 0.00 | 0.00 | 0.00 | 5.88 | 5.88 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
11 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
42 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
43 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
44 | 0.00 | 2.70 | 2.70 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
CP8304 | 0.00 | 8.11 | 8.11 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |