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15.04.2020 | Original Article

Prevalence and molecular characterization of parechovirus A in children with acute gastroenteritis in Shenzhen, 2016–2018

verfasst von: Ya-Nan Zhu, Yu-Hui Ye, Zhen Zhang, Yan-Jie Wu, Long Chen, Jing Wang, Yi-Jun Tang, Jun Meng, Hai-Long Zhang, Gui-Fang Hu

Erschienen in: Archives of Virology | Ausgabe 6/2020

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Abstract

Parechovirus A (PeV-A), which causes a wide variety of diseases, is prevalent among young children. However, little is currently known about PeV-A infections in children with acute gastroenteritis in mainland China. In this study, we investigated the molecular epidemiology of acute gastroenteritis in Shenzhen, southern China, with an emphasis on PeV-A infections. A total of 1220 stool specimens from 1220 outpatient children under 5 years old with acute gastroenteritis were collected from January 2016 to December 2018. Viral RNA was detected by a real-time RT-PCR and PCR method. The PeV-A isolates were genotyped by sequencing the VP3/VP1 region. Of 1220 specimens, 148 (12.1%) were positive for PeV-A. The predominant genotype was PeV-A 1B (68.9%), followed by PeV-A 4 (12.2%), PeV-A 14 (6.1%), PeV-A 1A (5.4%), PeV-A 6 (2.7%), PeV-A 3 (2.7%) and PeV-A 5 (2.0%). It was found that 68.2% of PeV-A infections occurred in the summer and rainy months (June to September) in southern China. The majority of PeV-A-positive patients (97.3%) were younger than 24 months old. PeV-A coinfection with norovirus, rotavirus, astrovirus and adenovirus was found in thirty specimens (30/148, 20.3%), five specimens (5/148, 3.4%), five specimens (5/148, 3.4%), and two specimens (2/148, 1.4%), respectively. Coinfections with more than one other enteric virus were not observed in any of the PeV-A-positive specimens. Phylogenetic analysis revealed that the PeV-A isolates from Shenzhen were closely related to each other and to strains circulating in China, suggesting endemic circulation of PeV-A in China. The results of this study indicate that PeV-A is one of important pathogens of acute gastroenteritis in young children and that coinfection is a possible mode of PeV-A infection. PeV-A associated with acute gastroenteritis exhibited high genotypic diversity in Shenzhen, southern China.

Joki-Korpela P, Hyypia T (2001) Parechoviruses, a novel group of human picornaviruses. Ann Med 33(7):466–471. https://doi.org/10.3109/07853890109002095 CrossRefPubMed

Bottcher S, Obermeier PE, Diedrich S, Kabore Y, D’Alfonso R, Pfister H, Kaiser R, Di Cristanziano V (2017) Genome sequence of novel human parechovirus type 17. Genome Announc. https://doi.org/10.1128/genomea.01649-16 CrossRefPubMedPubMedCentral

Graul S, Bottcher S, Eibach D, Krumkamp R, Kasmaier J, Adu-Sarkodie Y, May J, Tannich E, Panning M (2017) High diversity of human parechovirus including novel types in stool samples from Ghanaian children. J Clin Virol 96:116–119. https://doi.org/10.1016/j.jcv.2017.10.008 CrossRefPubMed

Nix WA, Khetsuriani N, Penaranda S, Maher K, Venczel L, Cselko Z, Freire MC, Cisterna D, Lema CL, Rosales P, Rodriguez JR, Rodriguez W, Halkyer P, Ronveaux O, Pallansch MA, Oberste MS (2013) Diversity of picornaviruses in rural Bolivia. J Gen Virol 94(9):2017–2028. https://doi.org/10.1099/vir.0.053827-0 CrossRefPubMedPubMedCentral

Hyypia T, Horsnell C, Maaronen M, Khan M, Kalkkinen N, Auvinen P, Kinnunen L, Stanway G (1992) A distinct picornavirus group identified by sequence analysis. Proc Natl Acad Sci USA 89(18):8847–8851. https://doi.org/10.1073/pnas.89.18.8847 CrossRefPubMed

Harvala H, Simmonds P (2009) Human parechoviruses: biology, epidemiology and clinical significance. J Clin Virol 45(1):1–9. https://doi.org/10.1016/j.jcv.2009.03.009 CrossRefPubMed

Guo Y, Duan Z, Qian Y (2013) Changes in human parechovirus profiles in hospitalized children with acute gastroenteritis after a three-year interval in Lanzhou, China. PLoS One. https://doi.org/10.1371/journal.pone.0068321 CrossRefPubMedPubMedCentral

Tauriainen S, Martiskainen M, Oikarinen S, Lonnrot M, Viskari H, Ilonen J, Simell O, Knip M, Hyoty H (2007) Human parechovirus 1 infections in young children–no association with type 1 diabetes. J Med Virol 79(4):457–462. https://doi.org/10.1002/jmv.20831 CrossRefPubMed

Benschop KS, Schinkel J, Minnaar RP, Pajkrt D, Spanjerberg L, Kraakman HC, Berkhout B, Zaaijer HL, Beld MG, Wolthers KC (2006) Human parechovirus infections in Dutch children and the association between serotype and disease severity. Clin Infect Dis 42(2):204–210. https://doi.org/10.1086/498905 CrossRefPubMed

10.

Esposito S, Rahamat-Langendoen J, Ascolese B, Senatore L, Castellazzi L, Niesters HG (2014) Pediatric parechovirus infections. J Clin Virol 60(2):84–89. https://doi.org/10.1016/j.jcv.2014.03.003 CrossRefPubMed

11.

Boivin G, Abed Y, Boucher FD (2005) Human parechovirus 3 and neonatal infections. Emerg Infect Dis 11(1):103–105. https://doi.org/10.3201/eid1101.040606 CrossRefPubMedPubMedCentral

12.

Levorson RE, Jantausch BA (2009) Human parechoviruses. Pediatr Infect Dis J 28(9):831–832. https://doi.org/10.1097/INF.0b013e3181badb6a CrossRefPubMed

13.

Pajkrt D, Benschop KS, Westerhuis B, Molenkamp R, Spanjerberg L, Wolthers KC (2009) Clinical characteristics of human parechoviruses 4–6 infections in young children. Pediatr Infect Dis J 28(11):1008–1010. https://doi.org/10.1097/INF.0b013e3181a7ab5f CrossRefPubMed

14.

Shan TL, Guo W, Cui L, Shang XG, Dai XQ, Yuan CL, Yu Y, Zhang W, Zhu JG, Shen Q (2009) The first detection of human parechovirus infections in China. J Clin Virol 45(4):371–372. https://doi.org/10.1016/j.jcv.2009.05.033 CrossRefPubMed

15.

Chen H, Yao Y, Liu X, Xiao N, Xiao Y, Huang Y, Chen Q, Yu S (2014) Molecular detection of human parechovirus in children with acute gastroenteritis in Guangzhou, China. Arch Virol 159(5):971–977. https://doi.org/10.1007/s00705-013-1915-0 CrossRefPubMed

16.

Chen HF, Zheng XY, Chen XM, Shi TL, Yao YX, Yuan Q, Chen Q, Yu SY (2015) Diversity and recombination of human parechovirus in children with acute gastroenteritis in Guangzhou, China. J Med Virol 87(2):296–302. https://doi.org/10.1002/jmv.24030 CrossRefPubMed

17.

Chiang GPK, Chen Z, Chan MCW, Lee SHM, Kwok AK, Yeung ACM, Nelson EAS, Hon KL, Leung TF, Chan PKS (2017) Clinical features and seasonality of parechovirus infection in an Asian subtropical city, Hong Kong. PLoS One. https://doi.org/10.1371/journal.pone.0184533 CrossRefPubMedPubMedCentral

18.

Zhang DL, Jin Y, Li DD, Cheng WX, Xu ZQ, Yu JM, Jin M, Yang SH, Zhang Q, Cui SX, Liu N, Duan ZJ (2011) Prevalence of human parechovirus in Chinese children hospitalized for acute gastroenteritis. Clin Microbiol Infect 17(10):1563–1569. https://doi.org/10.1111/j.1469-0691.2010.03390.x CrossRefPubMed

19.

Zhong H, Lin Y, Sun J, Su L, Cao L, Yang Y, Xu J (2011) Prevalence and genotypes of human parechovirus in stool samples from hospitalized children in Shanghai, China, 2008 and 2009. J Med Virol 83(8):1428–1434. https://doi.org/10.1002/jmv.22114 CrossRefPubMed

20.

Kumthip K, Khamrin P, Ushijima H, Maneekarn N (2019) Enteric and non-enteric adenoviruses associated with acute gastroenteritis in pediatric patients in Thailand, 2011 to 2017. PLoS One. https://doi.org/10.1371/journal.pone.0220263 CrossRefPubMedPubMedCentral

21.

Nix WA, Maher K, Johansson ES, Niklasson B, Lindberg AM, Pallansch MA, Oberste MS (2008) Detection of all known parechoviruses by real-time PCR. J Clin Microbiol 46(8):2519–2524. https://doi.org/10.1128/jcm.00277-08 CrossRefPubMedPubMedCentral

22.

Harvala H, Robertson I, McWilliam Leitch EC, Benschop K, Wolthers KC, Templeton K, Simmonds P (2008) Epidemiology and clinical associations of human parechovirus respiratory infections. J Clin Microbiol 46(10):3446–3453. https://doi.org/10.1128/jcm.01207-08 CrossRefPubMedPubMedCentral

23.

Tippmann HF (2004) Analysis for free: comparing programs for sequence analysis. Brief Bioinform 5(1):82–87. https://doi.org/10.1093/bib/5.1.82 CrossRefPubMed

24.

Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22(22):4673–4680. https://doi.org/10.1093/nar/22.22.4673 CrossRefPubMedPubMedCentral

25.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739. https://doi.org/10.1093/molbev/msr121 CrossRefPubMedPubMedCentral

26.

Patil PR, Ganorkar NN, Gopalkrishna V (2018) Epidemiology and genetic diversity of human parechoviruses circulating among children hospitalised with acute gastroenteritis in Pune, Western India: a 5-years study. Epidemiol Infect 146(1):11–18. https://doi.org/10.1017/s095026881700262x CrossRefPubMed

27.

Pham NTK, Thongprachum A, Shimizu Y, Trinh QD, Okitsu S, Komine-Aizawa S, Shimizu H, Hayakawa S, Ushijima H (2019) Diversity of human parechovirus in infants and children with acute gastroenteritis in Japan during 2014–2016. Infect Genet Evol 75:104001. https://doi.org/10.1016/j.meegid.2019.104001 CrossRefPubMed

28.

Malasao R, Khamrin P, Kumthip K, Ushijima H, Maneekarn N (2019) Molecular epidemiology and genetic diversity of human parechoviruses in children hospitalized with acute diarrhea in Thailand during 2011–2016. Adv Virol 164(7):1743–1752. https://doi.org/10.1007/s00705-019-04249-2 CrossRef

29.

Han TH, Kim CH, Park SH, Chung JY, Hwang ES (2011) Detection of human parechoviruses in children with gastroenteritis in South Korea. Adv Virol 156(8):1471–1475. https://doi.org/10.1007/s00705-011-0995-y CrossRef

30.

Baumgarte S, de Souza Luna LK, Grywna K, Panning M, Drexler JF, Karsten C, Huppertz HI, Drosten C (2008) Prevalence, types, and RNA concentrations of human parechoviruses, including a sixth parechovirus type, in stool samples from patients with acute enteritis. J Clin Microbiol 46(1):242–248. https://doi.org/10.1128/jcm.01468-07 CrossRefPubMed

31.

Chieochansin T, Vichiwattana P, Korkong S, Theamboonlers A, Poovorawan Y (2011) Molecular epidemiology, genome characterization, and recombination event of human parechovirus. Virology 421(2):159–166. https://doi.org/10.1016/j.virol.2011.09.021 CrossRefPubMed

32.

Ghazi F, Ataei Z, Dabirmanesh B (2012) Molecular detection of human parechovirus type 1 in stool samples from children with diarrhea. Int J Infect Dis 16(9):673–676. https://doi.org/10.1016/j.ijid.2012.05.1020 CrossRef

33.

Erhunmwunse IP, Eghafona Nosakhare O (2015) Viral agents of diarrhea in young children in two primary health centers in Edo State, Nigeria. Int J Microbiol 2015:1–5. https://doi.org/10.1155/2015/685821 CrossRef

34.

Harvala H, Wolthers KC, Simmonds P (2010) Parechoviruses in children: understanding a new infection. Curr Opin Infect Dis 23(3):224–230CrossRef

35.

Huang YP, Hsieh JY, Wu HS, Yang JY (2016) Molecular and epidemiological study of human parechovirus infections in Taiwan, 2007–2012. J Microbiol Immunol Infect 49(3):321–328. https://doi.org/10.1016/j.jmii.2014.06.013 CrossRefPubMed

36.

Watanabe K, Hirokawa C, Tazawa T (2016) Seropositivity and epidemiology of human parechovirus types 1, 3, and 6 in Japan. Epidemiol Infect 144(16):3451–3460. https://doi.org/10.1017/S0950268816001795 CrossRefPubMed

37.

Aizawa Y, Saitoh A (2015) Human Parechoviruses. Uirusu 65(1):17–26. https://doi.org/10.2222/jsv.65.17 CrossRefPubMed

38.

Lai CC, Wu FT, Ji DD, Mu JJ, Yang JR, Chiu KT, Lin WY, Li CY, Fu YP, Chen WT, Lee BC, Jiang DD, Yen MY, Wu HS (2011) Gastroenteritis in a Taipei emergency department: aetiology and risk factors. Clin Microbiol Infect 17(7):1071–1077. https://doi.org/10.1111/j.1469-0691.2010.03377.x CrossRefPubMed

39.

Chen X, Shi T, Huang J, Xiao G, Huang J, Xiong Y, Li X, Chen H, Zheng X, Yu S, Chen Q (2018) Molecular detection and phylogenetic analysis of human parechovirus in individuals with acute diarrhea and healthy controls in Guangzhou, China. J Med Virol 90(9):1444–1452. https://doi.org/10.1002/jmv.25222 CrossRefPubMed

40.

Pham NTK, Sayaka T, Dinh Nguyen T, Quang Duy T, Chandra A, Asiri A, Pattara K, Shoko O, Hiroyuki S, Masashi M (2011) Human parechovirus infection in children hospitalized with acute gastroenteritis in Sri Lanka. J Clin Microbiol 49(1):364–366. https://doi.org/10.1128/JCM.02151-10 CrossRefPubMed

41.

Benschop K, Thomas X, Serpenti C, Molenkamp R, Wolthers K (2008) High prevalence of human Parechovirus (HPeV) genotypes in the Amsterdam region and identification of specific HPeV variants by direct genotyping of stool samples. J Clin Microbiol 46(12):3965. https://doi.org/10.1128/JCM.01379-08 CrossRefPubMedPubMedCentral

42.

Isabelle S, Etienne J, Yves G, Béatrice K, Geneviève B, Daniel F, Bruno L, Florence M (2012) Human parechovirus infections, Lyon, France, 2008–2010: evidence for severe cases. J Clin Virol 54(4):337–341. https://doi.org/10.1016/j.jcv.2012.04.016 CrossRef

43.

Nix WA, Maher K, Pallansch MA, Oberste MS (2010) Parechovirus typing in clinical specimens by nested or semi-nested PCR coupled with sequencing. J Clin Virol 48(3):202–207. https://doi.org/10.1016/j.jcv.2010.04.007 CrossRefPubMed

Titel: Prevalence and molecular characterization of parechovirus A in children with acute gastroenteritis in Shenzhen, 2016–2018
verfasst von: Ya-Nan Zhu
Yu-Hui Ye
Zhen Zhang
Yan-Jie Wu
Long Chen
Jing Wang
Yi-Jun Tang
Jun Meng
Hai-Long Zhang
Gui-Fang Hu
Publikationsdatum: 15.04.2020
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 6/2020
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04587-6

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Prevalence and molecular characterization of parechovirus A in children with acute gastroenteritis in Shenzhen, 2016–2018

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