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Clinical Oral Investigations

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23.12.2015 | Original Article

Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota—a clinical study

verfasst von: A. Al-Ahmad, T. M. Auschill, R. Dakhel, A. Wittmer, K. Pelz, C. Heumann, E. Hellwig, N. B. Arweiler

Erschienen in: Clinical Oral Investigations | Ausgabe 8/2016

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Abstract

Objectives

The correlation between caries and the oral prevalence of Candida spp. in children is contradictory in literature. Thereby, authors focused on Candida albicans as the most isolated Candida species from the oral cavity. Therefore, the aim of the present study was to compare caries-free and caries-bearing children regarding their oral carriage of Candida spp.

Material and methods

Twenty-six caries-free (CF group) and 26 caries-active children (CA group) were included into this study. Three different types of specimens were assessed, saliva and plaque, and in the case of caries, infected dentine samples were microbiologically analyzed for aerobic and anaerobic microorganisms and their counts. Special attention was given to the differentiation between C. albicans and Candida dubliniensis. Additionally, different biochemical tests, VITEK 2 (VITEK®2, bioMérieux, Marcy-l’Etoile, France) and 16S and 18S ribosomal DNA (rDNA) sequencing, were applied for identification.

Results

The detection of C. albicans did not differ between the CF and CA groups. C. dubliniensis was never detected in any specimen of the CF group, but occurred in one quarter of the CA group (27 % in plaque, 23 % in saliva), thus leading to a statistically significant difference between the two groups (p < 0.05). In six of these cases, C. dubliniensis was detected concomitantly in saliva and plaque and once only in plaque. CA group harbored statistically more Streptococcus mutans than the control group revealing a correlation between S. mutans and C. dubliniensis regarding the caries group.

Conclusions

This is the first study reporting a frequent detection of C. dubliniensis in caries-active children, which could have been underestimated so far due to difficulties in differentiation between this yeast species and C. albicans.

Clinical relevance

Microbiological diagnostic—especially of oral Candida species—is an important determinant for identifying etiological factors of dental caries in children.

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Titel: Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota—a clinical study
verfasst von: A. Al-Ahmad
T. M. Auschill
R. Dakhel
A. Wittmer
K. Pelz
C. Heumann
E. Hellwig
N. B. Arweiler
Publikationsdatum: 23.12.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 8/2016
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1696-9

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Prevalence of Candida albicans and Candida dubliniensis in caries-free and caries-active children in relation to the oral microbiota—a clinical study

Abstract

Objectives

Material and methods

Results

Conclusions

Clinical relevance

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Abstract

Objectives

Material and methods

Results

Conclusions

Clinical relevance

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