Prevalence of sleep disturbance and the association between poor disease control in people with ankylosing spondylitis within the Australian clinical setting (ASLEEP study): a real-world observational study using the OPAL dataset

This is a cross-sectional study of observational data embedded within the OPAL dataset. The OPAL dataset contains de-identified information captured during routine clinical care using purpose-built worksheets in the Audit4 electronic medical record (EMR) [11, 12]. A protocol for this study was developed and approved by the institutional ethics committee prior to analysis. The protocol was not registered on the Open Science Framework, nor was it published. A statistical analysis plan was also developed and approved prior to analysis. No post hoc changes to the statistical analysis plan were made.

Data from patients with a physician diagnosis of AS (ICD code M45, M45.0 or M08.1) in the OPAL dataset, were alive at the time of the data cut and between 18 and 95 years and had completed at least one Insomnia Severity Index (ISI) or Multivariate Apnoea Prediction Index (MAPI) questionnaire between 1 January 2019 and 30 September 2020 were included in the study. Variables used in this study included the patient’s demographics (sex, age, body mass index, symptom duration [defined as the date of sleep questionnaire administration minus the date of onset of symptoms], line of biologic therapy), disease severity (Bath Ankylosing Spondylitis Disease Activity Index [BASDAI], erythrocyte sedimentation rate [ESR], c-reactive protein [CRP]), treatment (corticosteroid, NSAID, bDMARD), the presence of co-morbid depression (ICD codes F06.32, F10.8, F11.8, F13.8, F14.8, F15.8, F16.8, F18.8, F19.8, F32, F33 or F34.1) and responses to patient-reported outcome questionnaires on sleep (ISI, MAPI, Functional Assessment of Chronic Illness Therapy—Fatigue [FACIT-Fatigue]). Follow-up information was collected until 30 September 2020.

The activities of OPAL Rheumatology Ltd have received overarching ethics approval from the University of NSW Human Research Ethics Committee (UNSW HREC), based on a patient opt-out arrangement (HC17799). This research protocol was approved by the UNSW HREC (HC180712).

Validated PROs (ISI, MAPI, FACIT-Fatigue) were sent from the patients EMR within Audit4 and delivered to the patient’s email address at time intervals specified by their treating rheumatologist or completed in the clinic waiting room using a tablet or the patient’s smart phone. Completed questionnaires were encrypted and returned to the patient’s Audit4 EMR for review at the next consultation [12].

The primary objective was to determine the prevalence of sleep disorders as measured by the number of patients with AS who were identified as being at risk of insomnia or obstructive sleep apnoea using the ISI or MAPI during the index period divided by the total number of patients with AS who had completed a survey.

The secondary objectives were to assess the relationship between demographic factors and sleep disturbance; to assess the impact of therapy for AS on sleep outcomes; to assess the prevalence of sleep disturbance in patients by AS therapy, specifically treatment with a tumour necrosis factor inhibitor (TNFi) or an interleukin 17A inhibitor (IL-17Ai), at index; and to assess the correlation between clinical signs and symptoms (measured by the BASDAI and FACIT-Fatigue), with sleep disturbance.

All patients with clinical data in the OPAL dataset that met the inclusion/exclusion criteria were included in the study. Assuming that 500 patients completed the questionnaires (of an approximate patient population with AS in the OPAL dataset of 3,800), then if the prevalence of sleep disturbance was 65% in either treatment group (based on previously published data [13]), the confidence interval around IL-17Ai users was calculated to be 51.2 to 78.8% and around TNFi users was calculated to be 60.5 to 69.5%. This assumes that IL-17Ai users made up 10% of the PRO completers, and TNFi users made up 90% of the PRO completers. The proportions of patients prescribed IL-17Ai vs TNFi was based analysis of prescribing patterns from the OPAL dataset.

Due to the potential biases in observational data, secondary analyses used a propensity score matching method to increase the comparability of the observed index characteristics of patients treated with IL-17Ai compared to TNFi. The propensity score is the conditional probability of receiving treatment (that is IL-17Ai vs TNFi) which was estimated using linear regression. We planned to use age group at index, sex, BASDAI score, duration of disease, concomitant therapy and line of therapy as covariates in the model. We planned to use a ratio of one patient prescribed IL-17Ai to every ten patients prescribed TNFi, which reflected the current treatment usage patterns at the time of study initiation. We assessed the success of matching by comparing the standardized differences in means and proportions between groups, with differences of more than 0.1 considered substantial differences [14]. We were unable to find suitable matches on all the planned covariates due to missing data or inadequate matching; therefore, the propensity score matching was conducted using sex, age category and disease duration. BASDAI category was missing for 183 (37%) of patients, and suitable matches on concomitant medication and line of therapy were not possible. We used a ratio of one user of IL-17Ai to every two users of TNFi. Further matching was not possible due to inadequate matches being available within the dataset.

Patients were defined as having no clinically significant insomnia (ISI score ≤ 7), subthreshold insomnia (ISI > 7 and ≤ 14), moderate severity clinical insomnia (ISI score > 14 and ≤ 21) or severe clinical insomnia (ISI score > 21) [15]. Patients were considered to have poor disease control if their BASDAI score was ≥ 4 [16]. The MAPI is a score for predicting sleep apnoea in patients [17]. Scores range from 0 to 1, with higher scores associated with an increased probability of sleep apnoea. MAPI values higher than 0.5 are suggestive of clinical apnoea [17].

The association of demographic factors on sleep disturbance (ISI category, MAPI) was assessed using ordinal or logistic regression (as appropriate). Covariates included in the model were age (category), sex, weight, body mass index (BMI), symptom duration, BASDAI score (< 4 vs ≥ 4) and use of steroids (no vs yes), non-steroidal anti-inflammatory drugs (NSAIDs; no vs yes) and bDMARDs (IL-17Ai vs TNFi).

Of the 5,323 patients with AS in the OPAL dataset, 629 (11.8%) were sent sleep questionnaires and 495 (9.3%) were included in the study (79% response rate), and the demographics are reported in Table 1. Of the 495 patients, 48 (9.7%) were receiving IL-17Ai, 395 (79.8%) were receiving TNFi and 52 (10.5%) were receiving other biological and non-biological treatments. For patients receiving TNFi, the majority were receiving adalimumab (n = 167/395, 42.3%) or etanercept (n = 92/395, 23.3%). A greater proportion of women were receiving IL-17Ai inhibitors compared to TNFi or other treatments (64.6%, 41.8% and 46.2%, respectively, p < 0.001), and less patients in the IL-17Ai group were receiving the drug as a first line biologic treatment compared to TNFi (64.6% vs 85.3%, p = 0.01). Median CRP was lower in the IL-17Ai group compared to other groups (2.0 in IL-17Ai compared to 3.0 in TNFi or 3.4 in other treatment, p = 0.026). There were no other clinically relevant differences in index demographics in the overall population.

In the propensity score matched set, 48 patients were prescribed IL-17Ai at index, and 94 were prescribed TNFi at index (Table 2). Patients in each group were well matched, with the exception of line of therapy, where there was a higher proportion of TNFi users in first line of bDMARD therapy compared to IL-17Ai users (82% vs 65%, p = 0.034).

At index, the mean ISI total score in the overall population was 8.6 ± 6.2. Moderate or severe clinical insomnia based on the ISI was present in 16% and 3.2% of patients, respectively. This was largely driven by reported difficulties staying asleep, dissatisfaction with sleep and sleep interference. The mean MAPI score at index was 0.4 ± 0.3, with 31.5% of evaluable patients (that is patients with a non-missing MAPI questionnaire) identified as being at risk of sleep apnoea. Few patients self-reported stopping breathing (rarely 10.9%, sometimes 5.1%, frequently 1.6% or always 1.6%). Moderate or severe clinical insomnia based on the ISI and a risk of sleep apnoea based on MAPI score > 0.5 was reported in 5.6% of patients. The mean FACIT-Fatigue total score at index was 36.1 ± 10.7. The greatest difficulties reported in FACIT-Fatigue subscales were with fatigue, tiredness, energy levels and interference with usual activities.

In the overall population, there were statistically significant differences in the proportion of patients reporting the following symptoms on the ISI: difficulty staying asleep (severe or very severe problems: IL-17Ai 14.6%, TNFi 9.6%, other 26.9%, p = 0.002); satisfaction with their sleep (dissatisfied or very dissatisfied: IL-17Ai 20.8%, TNFi 23.5%, other 40.4%, p = 0.011); experiencing sleep interference (much or very much: IL-17Ai 18.7%, TNFi 14.7%, other 27.2%, p < 0.001); problems being noticeable to others (much or very much: IL-17Ai 12.5%, TNFi 8.8%, other 21.1%, p = 0.007); and worry or distress (much or very much: IL-17Ai 10.4%, TNFi 9.9%, other 12.4%, p = 0.012). However, there was no difference between the groups in the proportion of patients with clinically suspected moderate or severe insomnia (IL-17Ai 20.9%, TNFi 16.0%, other 28.9%, p = 0.079).

There were some treatment-related differences in the propensity score matched population: the mean MAPI index was higher in those with IL-17Ai at index (IL-17Ai 0.4 ± 0.3 and TNFi 0.3 ± 0.2, p = 0.046), and the proportion at risk of sleep apnoea based on the MAPI score was higher in those treated with IL-17Ai than those treated with TNFi (66% vs 54%, p = 0.051), although this did not reach statistical significance.

In the propensity score matched population, those with poor disease control (BASDAI ≥ 4) were seven times more likely (odds ratio [OR] 7.29, 95% CI 2.37 to 22.46, p = 0.001) to have a greater severity of insomnia symptoms as measured by ISI category than those with good disease control. Those taking steroids at index were almost four times more likely (OR 3.98, 95% CI 1.03 to 15.34, p = 0.045) to experience greater sleep disturbance than those not taking steroids. There were 87 patients included in this model. No other factors were significant (Table 3). It should be noted that model fit was relatively poor (McFadden’s pseudo R-squared 0.2516), the model is likely over specified (that is the number of covariates in the model is not supported by the number of patients included in it), and there were wide confidence intervals.

In the MAPI model, females were less likely (OR 0.00, 95% CI 0.00 to 0.13, p < 0.001) to be at risk of sleep apnoea; those reported as taking NSAIDs within the EMR were 12 times more likely to be at risk of sleep apnoea (OR 12.1, 95% CI 1.42 to 103.1, p = 0.022) compared to those not taking NSAIDs. No other factors were significant. Again, model fit was poor (McFadden’s pseudo R-squared 0.5518), and for some factors there are extremely wide confidence intervals, and the model is likely over specified (that is the number of covariates in the model is not supported by the number of patients within the model as it included only 83 patients).

In patients administered TNFi at index, there were significant correlations between BASDAI and FACIT-Fatigue (p < 0.0001), BASDAI and ISI total score (p < 0.0001), and FACIT-Fatigue and ISI scores. In those treated with IL-17Ai, only the relationship between MAPI score and BASDAI scores (p = 0.04) and FACIT-Fatigue and ISI scores (p = 0.0001) were significant (Table 4).

In the both IL-17Ai and TNFi populations, MAPI score was significantly negatively correlated with response to BASDAI question 6 ‘how long does your morning stiffness last from the time you wake up’ (IL-17Ai − 0.45, p = 0.02; TNFi − 0.38, p < 0.0001). FACIT-Fatigue score was negatively correlated with BASDAI question 4 ‘how would you describe the overall level of discomfort you have had from any areas tender to touch or pressure’ (IL-17Ai: − 0.42, p = 0.03; TNFi: − 0.46, p < 0.0001). Insomnia symptoms as measured by ISI category were significantly associated with all BASDAI questions (Table 5).

In this subset of patients with AS, 19.2% reported moderate to severe clinical insomnia, and 31.5% were identified as at risk of sleep apnoea. We found no significant differences in the incidence of insomnia or sleep apnoea by treatment type in our matched population (TNFi vs IL-17Ai), with the exception of the MAPI score which was significantly higher in those treated with IL-17Ai. Those with poor disease control were more likely to experience greater sleep disturbance than those with good disease control measured by BASDAI.

The proportions of patients who reported moderate to severe clinical insomnia or identified as at risk of sleep apnoea are within the range of previously reported estimates among AS patients [3, 4], but higher, particularly with respect to apnoea, than typical for healthy populations [18, 19]. The high prevalence of insomnia and sleep apnoea in this real-world population of patients with AS highlights the importance of actively assessing patients in routine clinical care for signs of sleep disorders and referring patients to sleep physicians for further assessment and treatment where indicated. Disturbed sleep, including multiple nocturnal awakenings, early morning awakening, sleep onset insomnia, difficulty awakening and daytime malfunctioning can leave patients with AS feeling fatigued [20]. These sleep disorders are noted to be more severe than those experienced by patients without AS [1]. While the present study was not designed to consider the impact of commencement of treatment for sleep disorders in people with AS, treatment of sleep disorders, such as sleep apnoea, has been associated with improvements in AS [21]. Treatment of the underlying AS can also improve sleep disturbance: for example, treatment with TNFi has been reported to improve sleep disturbance in patients with AS [22], although others report that improvements are only with sleep quality, and treatment with TNFi does not improve sleep measured via polysomnography [23]. This is possibly because the specific impact of AS on sleep depends on the underlying symptomology of AS. For example, pain is associated with poor sleep efficiency (the proportion of time attempting sleep that is actually spent asleep), while elevated CRP is associated with longer sleep latency [1]. TNFα and IL-17A have been reported to be important in the pathogenesis of obstructive sleep apnoea; it is possible that TNFα is more strongly associated with its development [24, 25].

We found that those with poor disease control were more likely to experience greater sleep disturbance than those with good disease control measured by BASDAI. This may reflect lesser control of the underlying inflammatory condition (for example levels of TNFα and IL-17A). There is a bidirectional relationship between sleep disturbance and disease control [20]: sleep disorders may contribute to emerging pain disorders, but pain may also lead to or exacerbate sleep disorders [26]. Unsurprisingly, we found that those patients taking concomitant steroids had increased levels of sleep disturbance. This is a well characterized association [27].

We also found that men with AS were at higher risk of sleep apnoea, which reflects the known sex epidemiology of sleep apnoea [28]. Others have reported that two to three times more men without AS are diagnosed with obstructive sleep apnoea compared to women [28]. We found no association between sleep disturbances and BMI, although this was likely confounded by sex, and the relationship between BMI and severity of obstructive sleep apnoea is likely complex [28].

Strengths of our study include that it is based on real-world clinical evidence collected at the point of care for patients with ankylosing spondylitis in Australia. The OPAL dataset is one of the largest datasets of patients with rheumatic diseases in the world [12]. In addition, OPAL captures multifaceted outcomes data from the patient perspective through a novel electronic patient-reported outcome (ePRO) delivery system which allows for health-related quality of life measures to be matched with clinical indices [12]. The patient-reported outcomes included in this study are clinically relevant for the initial assessment of sleep disturbance and sleep apnoea.

Our study has some potential limitations. This is a retrospective study based on data captured during routine clinical care. The analysis is therefore limited by the availability of data for secondary research in the dataset. The presence of missing data may also impact on the analysis; sample size, variables and study duration were selected to minimize the impact of missing data. Of the available patients with AS, only 9.3% were included in this study. Our assessment of sleep disorders was based on self-reported responses to sleep questionnaires and objective assessment via polysomnography, for example, was not conducted. It is possible therefore that the true estimate of sleep disorders may be lower or higher than that observed in our analysis. Additionally, the BASDAI is a subjective questionnaire, and further studies are needed to evaluate the association between sleep disturbance and objective signs of disease activity such as the ASDAS or c-reactive protein levels. Our regression modelling was hampered by poor fit, likely due to a combination of low sample sizes, and that sleep disturbance in this real-world population is likely multi-factorial and requires further investigation. For example, we did not assess the influence of exercise/physical activity on sleep as this information is not available in the dataset. Finally, we made no adjustments for multiple comparisons, and as such, it is possible that some of our statistically significant findings occurred by pure chance.