Ahmed Mahmoud, Nadine A Sherif, Rana Abdella, Amira R El-Genedy, Abdalla Y El Kateb and Ahmed NH Askalani contributed equally to this work.
The authors declare that they have no competing interests, whether financial, or non-financial.
AM carried out the data collection and sampling from the patients. NAS carried out sampling from the patients, drafting of the manuscript, and revising it. RA carried out the analysis of the data, and final approval of the version to be published. ARE carried out the test done for the T. vaginalis (wet mount, Giemsa stain, kalon test and Diamond test) with their interpretation. Both AYE and ANHA carried out the data collection.All authors read and approved the final version.
This is a cross-sectional study carried out in the Obstetrics and Gynecology Department at Kasr Al- Ainy Cairo University Hospitals.
One thousand female patients in the child bearing period (age 18-45 yrs) were included in this study. These females were non-pregnant and non-menstruating with no douching or intercourse for at least 2–3 days, no use of antibiotics, anti-protozoal or steroids for the past 15 days complaining of vaginal discharge with or without itching, burning sensation or both. Vaginal swabs were obtained from all patients for examination by direct wet mount examination, Giemsa staining, Modified Diamond culture and latex agglutination test Kalon) to detect the presence of Trichomonas vaginalis infection.
The prevalence of trichomonas infection was 50 cases, latex agglutination test detected 50 positive cases, 30 of which were also positive by culture, and only 10 were detected both by Giemsa staining and by wet mount.
The wet mount, Giemsa staining and Kalon latex test had sensitivities of 33.3, 33.3% and 100% respectively while their specificities were 100%, 100% and 97.9% respectively.
Screening tests should be done routinely to depict cases of T. vaginalis infection and should be included in the control programs of sexually transmitted infections. Although wet mount is not a sensitive method for diagnosis of T. vaginalis yet, it is a good positive one. Staining is only useful when there is heavy T. vaginalis infection.
Latex agglutination is a highly sensitive, simple, rapid and cost effective test. It provides results within 2-3 minutes and it has the potential for use in screening and diagnosis of T. vaginalis infection.
Kaul P, Gupta I, Sehgal R, Malla N. Trichomonas vaginalis: random amplified polymorphic DNA analysis of isolates from symptomatic and asymptomatic women in India. Parasitol Int. 2004;262:253–5.
Mabey D, Ackers J, Adu-Sarkodie Y. Trichomonas vaginalis infection. Sex Transm Infect. 2006;82(S4):26–7.
World Health Organization. Prevalence and incidence of selected sexually transmitted infections, Chlamydia trachomatis, Neisseria gonorrhea, syphilis and Trichomonas vaginalis: methods and results used by WHO to generate 2005 estimates. Geneva, Switzerland: WHO Press; 2011.
Soper D. Trichomoniasis: under control or undercontrolled? Am J Obst Gynecol. 2004;190:281–90. CrossRef
Ojuromi O, Oyibo W, Tayo A, Ibidapo A, Fagbenro-Beyioku FA, Oladosu OO, et al. Reliance on microscopy in Trichomonas vaginalis diagnosis and its prevalence in females presenting with vaginal discharge in Lagos, Nigeria. J Infect Dev Ctries. 2007;1(2):210–3.
Van Der Pol B, Kwok C, Louis PB, Rinaldi A, Salata RA, Chen P, et al. Trichomonas vaginalis infection and human immunodeficiency virus acquisition in African Women. J Infect Dis. 2008;197:548–54. CrossRef
AI-Saeed WM. Detection of Trichomonas vaginalis by different methods in women from Dohok province, Iraq. East Mediterr Health J. 2011;17(9):706–9.
Silva LC, Miranda AE, Batalha RS, Monte RL, Talhari S. Trichomonas vaginalis and associated factors among women living with HIV/AIDS in Amazonas, Brazil. Trans R Soc Med Hyg. 2013;13:164–5.
Trevisan A, Mengoli C, Rossi L, Cattai M, Cavallaro A. Epidemiology of reproductive tract infections in asymptomatic population of North-East of Italy. Minerva Gynecol. 2008;60(2):135–42.
Darani HY, Firuzeh A, Nozhat Z, Hossein AY, Hedayat S. Development of a Latex Agglutination Test as a Simple and Rapid Method for Diagnosis of Trichomonas vaginalis Infection. Avicenna J Med Biotechnol. 2010;1(2):63–6.
- Prevalence of Trichomonas vaginalis infection among Egyptian women using culture and Latex agglutination: cross-sectional study
Nadine A Sherif
Amira R El-Genedy
Abdalla Y El Kateb
Ahmed NH Askalani
- BioMed Central
Neu im Fachgebiet Gynäkologie und Geburtshilfe
Meistgelesene Bücher aus dem Fachgebiet
e.Med Kampagnen-Visual, Mail Icon II