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European Journal of Nutrition

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01.06.2010 | Original Contribution

Prevention of oxidative DNA damage in inner organs and lymphocytes of rats by green tea extract

verfasst von: Nina Kager, Franziska Ferk, Michael Kundi, Karl-Heinz Wagner, Miroslav Mišík, Siegfried Knasmüller

Erschienen in: European Journal of Nutrition | Ausgabe 4/2010

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Abstract

Background

Consumption of green tea (GT) is associated with decreased incidences of specific forms of cancer in humans and it was postulated that its antioxidant (AO) properties may account for these effects. The evidence for AO effects of GT is mainly based on the results from in vitro experiments and on animal studies in which protection against chemically induced damage was monitored.

Aim of the study

The goal of the study was the investigation of the prevention of strand breaks and DNA migration attributable to endogenous oxidation of bases by GT extract (GTE) in inner organs and lymphocytes of untreated rats. In addition, immunological parameters and biochemical markers were monitored.

Methods

DNA migration was measured in hepatocytes, colonocytes and lymphocytes after consumption of a low (1.3 mg/kg bw per day, 5 days) and a high dose (6.5 mg/kg bw per day, 5 days) of GTE in COMET assays (n = 5 animals per group). In addition, immunological parameters (TNF-α, IFN-γ, IL-4 and IL-10), the total AO capacity and oxidized low-density lipoproteins were determined in plasma.

Results

No evidence for reduction in DNA damage was found with a lower dose, whereas with the higher dose, reduction in DNA migration attributable to formamidopyrimidine-DNA-glycosylase sensitive lesions (oxidized purines) and endonuclease III-sensitive sites (oxidized pyrimidines) (58 and 73%) was observed in lymphocytes; also, in colonocytes (reduction in FPG-sensitive sites by 46%) and hepatocytes (decrease in Endo III-sensitive sites by 74%) protective effects were found, while none of the other parameters was altered.

Conclusions

Our results show that a dose of GTE, which is equivalent to consumption of 500 ml GT/p/day in humans protects lymphocytes and to a lesser extent inner organs against oxidative DNA damage, while no effect was seen with a lower dose corresponding to an uptake of 100 ml/p/day.

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Titel: Prevention of oxidative DNA damage in inner organs and lymphocytes of rats by green tea extract
verfasst von: Nina Kager
Franziska Ferk
Michael Kundi
Karl-Heinz Wagner
Miroslav Mišík
Siegfried Knasmüller
Publikationsdatum: 01.06.2010
Verlag: Springer-Verlag
Erschienen in: European Journal of Nutrition / Ausgabe 4/2010
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-009-0068-0

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Prevention of oxidative DNA damage in inner organs and lymphocytes of rats by green tea extract

Abstract

Background

Aim of the study

Methods

Results

Conclusions

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Abstract

Background

Aim of the study

Methods

Results

Conclusions

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