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Horm Metab Res 2010; 42(7): 514-520
DOI: 10.1055/s-0030-1249639
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Effects of 17β-Estradiol on Preadipocyte Proliferation in Human Adipose Tissue: Involvement of IGF1-R Signaling

E. Dos Santos1 , M.-N. Dieudonné1 , M.-C. Leneveu1 , V. Sérazin1 , V. Rincheval2 , B. Mignotte2 , E. Chouillard3 , P. De Mazancourt1 , Y. Giudicelli1 , R. Pecquery1
  • 1Service de Biochimie et Biologie Moléculaire, UPRES EA 2493, Faculté de Médecine Paris-Ile de France Ouest, Université Versailles St Quentin, PRES Universud Paris, Centre Hospitalier de Poissy, Poissy, France
  • 2Laboratoire de Génétique et Biologie cellulaire, UMR 8159 CNRS, Université Versailles St Quentin, PRES Universud Paris, France
  • 3Service de Chirurgie Viscérale, Centre Hospitalier de Poissy, Poissy, France
Further Information

Publication History

received 27.08.2009

accepted 23.02.2010

Publication Date:
08 April 2010 (online)

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Abstract

Estrogens are known to stimulate the proliferation of human preadipocytes. However, the molecular mechanisms underlying the increased cell growth by these steroids are poorly understood. In the present study, we have demonstrated that the proliferative effect of 17β-estradiol involves the induction of both cell cycle gene expressions, c-myc and cyclin D1. Moreover, the mitogenic effects of 17β-estradiol are suppressed by the pure antagonist ICI 182780 suggesting that estradiol action is mediated by estrogen receptor (ER). We have also shown that 17β-estradiol is able to inhibit human preadipocyte apoptosis capacity as reflected by DNA fragmentation experiments and the mRNA expression of the pro- and antiapoptotic genes. Finally, 17β-estradiol significantly induces both mRNA and protein expression of RIGF1 in human preadipose cells via ER and thus reinforces the signaling pathway of the proliferative factor, IGF1. Taken together, these data reinforce the concept of cross-talk between IGF1- and ER-signaling pathways in preadipocytes and indicate that IGFI may be a critical regulator of estrogen-mediated preadipose growth.

Key words

human adipose tissue - estrogens - proliferation - IGF1 receptor - apoptosis

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Correspondence

E. Dos Santos

Service de Biologie Médicale

Centre Hospitalier

78303 Poissy Cedex

France

Phone: +33/139/27 51 40

Fax: +33/139/27 44 20

Email: biochip@wanadoo.fr

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