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Planta Med 2001; 67(3): 274-276
DOI: 10.1055/s-2001-12002
Letter

© Georg Thieme Verlag Stuttgart · New York

Effect of Genistein Analogs on Oxygen Radical Production in Leukocytes Stimulated by Unopsonized Zymosan

Jieun Yun1 , Sei Ryang Oh2 , Hyeong-Kyu Lee2 , IL-Moo Chang3 , Kiyoshi Takatsu4 , Yeo-Pyo Yun1 , Kyung Rak Min1 , Youngsoo Kim1,*
  • 1 College of Pharmacy, Chungbuk National University, Cheongju, Korea
  • 2 Korea Research Institute of Bioscience & Biotechnology, Taejon, Korea
  • 3 Natural Products Research Institute, Seoul National University, Seoul, Korea
  • 4 Institute of Medical Science, University of Tokyo, Tokyo, Japan
Further Information

Publication History

April 18, 2000

July 21, 2000

Publication Date:
31 December 2001 (online)

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Abstract

Effects of genistein analogs on oxygen radical production have been analyzed in human neutrophils, human monocytes or murine macrophages Raw264.7 stimulated with unopsonized zymosan by lucigenin- and luminol-enhanced chemiluminescence assays. Genistein exhibited IC50 values of 10.7 - 11.5 μM on the oxygen radical production in human neutrophils, 10.9 - 11.0 μM in human monocytes, and 14.8 - 27.3 μM in Raw264.7 cells. Orobol, a genistein analog with an additional hydroxy group at the 3′ position, exhibited IC50 values of 3.0 - 3.3 μM on the oxygen radical production in human neutrophils, 2.8 - 3.1 μM in human monocytes, and 1.5 - 3.9 μM in Raw264.7 cells. Genistin and sophoricoside are genistein glycosides with a glucose moiety at 7 or 4′ position, respectively. The genistein glycosides exhibited 23 - 37 % inhibitory effects at 100 μM on the oxygen radical production.

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Prof. Youngsoo Kim, Ph.D.

College of Pharmacy

Chungbuk National University

Cheongju 361-763

Republic of Korea

Email: youngsoo@cbucc.chungbuk.ac.kr

Fax: +82-43-268-2732

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