Effect of Mahonia aquifolium Active Compounds on Interleukin-8 Production in the Human Monocytic Cell Line THP-1

 

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Abstract

The effect of the crude extract and of two alkaloid fractions prepared from Mahonia aquifolium on interleukin-8 (IL-8) production in the lipopolysaccharide (LPS)-stimulated human monocytic cell line THP-1 was studied. The production of IL-8 by cells stimulated with 20 ng/ml LPS after 48 h treatment with 20 μg/ml crude extract was inhibited by about 30 %. LPS-stimulated cells treated with 0.1 μg/ml bisbenzylisoquinoline alkaloid fraction exhibited a 40 % inhibition of IL-8 production in comparison with control cells. The fraction of protoberberine alkaloids had no significant inhibitory activity. Weak or no inhibition of IL-8 production was found after treatment with 0.5 μg/ml of protoberberine alkaloids berberine and jatrorrhizine and with berbamine from the group of BBI alkaloids. In contrast, the production was inhibited after treatment with BBI alkaloids baluchistine (about 20 %) and aromoline (up to 30 %).

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Assoc. Prof. Dr. Daniela Košt’álová

Department of Pharmacognosy and Botany

Faculty of Pharmacy, Comenius University

Odbojárov 10

SK-832 32 Bratislava 3

Slovak Republic

Fax: +421-2-55572065

Email: kostalova@fpharm.uniba.sk