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Horm Metab Res 1993; 25(9): 457-461
DOI: 10.1055/s-2007-1002148
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© Georg Thieme Verlag, Stuttgart · New York

Metabolic Derangements in Excessive Insulin and Sulfonylurea Therapy

Mileni J. M. Ursich, Rosa T. Fukui, Dalva M. Rocha, Maria E. R. Silva, B. L. Wajchenberg
  • Laboratory of Medical Investigation (LIM 18) and Endocrine Service, Hospital das Clinicas of the University of São Paulo Medical School, São Paulo, Brazil
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Publication History

1992

1993

Publication Date:
14 March 2008 (online)

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Summary

Mild streptozotocin diabetic rats, characterized by normal or slighty elevated fasting blood glucose levels and glucose intolerance, treated with excessive doses of monocomponent pork insulin (0.5 U/day) (I) or glybenclamide (0.6 mg/day) (S) were compared to controls (C) and streptozotocin-diabetic rats without treatment (D). Intravenous glucose tolerance tests (0.75 g/kg) were performed in all animals and repeated after overtreatment. Insulin binding and insulin-induced D-(U-14C)-glucose transport and oxidation were also determined in isolated epididymal adipocytes. Diabetic rats showed a failure in the initial phase of insulin release and glucose intolerance as compared with (C). In overtreated rats glucose tolerance worsened (p<0.05) after therapy. Maximal insulin binding by isolated adipocytes at tracer insulin concentration was unchanged after excessive insulin or sulfonylurea therapy. Besides, glucose transport and oxidation in the cells of overtreated rats were greater than in D and even greater than in C. These apparently divergent results, i.e. deterioration of glucose tolerance with increased insulin action in adipocytes suggest that overtreatment induces a state of resistance to hormone action in other target tissue(s) than the adipose one, possibly muscle.

Key words

Hyperinsulinization - Streptozotocin Diabetes - Glucose Tolerance - Insulin Binding - Glucose Transport in Adipocytes

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