There is substantial evidence both in humans and in animals that a prolonged reduction in plasma thyroid hormone concentration leads to reproductive problems, including disturbed folliculogenesis, impaired ovulation and fertilization rates, miscarriage and pregnancy complications. The objective of the present study is to examine the consequences of chronic hypothyroidism, induced in adulthood, for the size of the ovarian follicle pool. In order to investigate this, adult female rats were provided either a control or an iodide deficient diet in combination with perchlorate supplementation to inhibit iodide uptake by the thyroid. Sixteen weeks later animals were sacrificed. Blood was collected for hormone analyses and ovaries were evaluated histologically.
At the time of sacrifice, plasma thyroid-stimulating hormone concentrations were 20- to 40-fold increased, thyroxine concentrations were negligible while tri-iothyronin concentrations were decreased by 40% in the hypothyroid group, confirming that the animals were hypothyroid. Primordial, primary and preantral follicle numbers were significantly lower in the hypothyroid ovaries compared to the euthyroid controls, while a downward trend in antral follicle and corpora lutea numbers was observed. Surprisingly the percentage of atretic follicles was not significantly different between the two groups, suggesting that the reduced preantral and antral follicle numbers were presumably not the consequence of increased degeneration of these follicle types in the hypothyroid group. Plasma anti-Müllerian hormone (AMH) levels showed a significant correlation with the growing follicle population represented by the total ovarian number of primary, preantral and antral follicles, suggesting that also under hypothyroid conditions AMH can serve as a surrogate marker to assess the growing ovarian follicle population.
The induction of a chronic hypothyroid condition in adult female rats negatively affects the ovarian follicular reserve and the size of the growing follicle population, which may impact fertility.
Joshi JV, Bhandarkar SD, Chadha M, Balaiah D, Shah R. Menstrual irregularities and lactation failure may precede thyroid dysfunction or goitre. J Postgrad Med. 1993;39:137–41. PubMed
Chai J, Yeung WYT, Lee CYV, Li HWR, Ho PC, Ng HYE. Live birth rates following in vitro fertilization in women with thyroid autoimmunity and/or subclinical hypothyroidism. Clin Endocrinol. 2014;80:122–27. CrossRef
Poppe K, Velkeniers B, Glinoer D. The role of thyroid autoimmunity in fertility and pregnancy. Nat Clin Pract Endocrinol Metab. 2008;4:393–405. CrossRef
Meng L, Rijntjes E, Swarts H, Bunschoten A, van der Stelt I, Keijer J, Teerds K. Dietary-induced chronic hypothyroidism negatively affects rat follicular development and ovulation rate and is associated with oxidative stress. Biol Reprod. 2016;94:1–11. CrossRef
Reeves PG, Nielsen FH, Fahey GC. Ain-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the Ain-76A rodent diet. J Nutr. 1993;123:1939–51. PubMed
Schroder-Van der Elst JP, Van der Heide D, De Escobar GM, Obregon MJ. Iodothyronine deiodinase activities in fetal rat tissues at several levels of iodine deficiency: A role for the skin in 3,5,3 ′-triiodothyronine economy? Endocrinology. 1998;139:2229–34. PubMed
Palm IF, van der Beek EM, Swarts HJ, van der Vliet J, Wiegant VM, Buijs RM, Kalsbeek A. Control of the estradiol-induced prolactin surge by the suprachiasmatic nucleus. Endocrinology. 2001;142:2296–302. CrossRef
Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL. Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature. 2004;428:145-150. Erratum in Nature. 2004;430:1062–62.
Jefferys A, Vanderpump M, Yasmin E. Thyroid dysfunction and reproductive health. Obstet Gynaecol. 2015;17:39–45. CrossRef
Practice Committee of the American Society for Reproductive M. Subclinical hypothyroidism in the infertile female population: a guideline. Fertil Steril. 2015;104:545–53. CrossRef
Magri F, Schena L, Capelli V, Gaiti M, Zerbini F, Brambilla E, Rotondi M, De Amici M, Spinillo A, Nappi RE, Chiovato L. Anti-Müllerian hormone as a protector of ovarian reserve in ART protocols: the hidden role of thyroid autoimmunity. Reprod Biol Endocrinol. 2015;13:106. CrossRefPubMedPubMedCentral
- Prolonged hypothyroidism severely reduces ovarian follicular reserve in adult rats
Hans J. M. Swarts
Katja J. Teerds
- BioMed Central
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