The online version of this article (doi:10.1186/s12985-017-0743-3) contains supplementary material, which is available to authorized users.
Changes in the levels of circulating microRNAs (miRNAs) in the serum of humans and animals have been detected as a result of infection with a variety of viruses. However, to date, such a miRNA profiling study has not been conducted for foot-and-mouth disease virus (FMDV) infection.
The relative abundance of 169 miRNAs was measured in bovine serum collected at three different phases of FMDV infection in a proof-of-concept study using miRNA PCR array plates.
Alterations in specific miRNA levels were detected in serum during acute, persistent, and convalescent phases of FMDV infection. Subclinical FMDV persistence produced a circulating miRNA profile distinct from cattle that had cleared infection. bta-miR-17-5p was highest expressed during acute infection, whereas bta-miR-31 was the highest during FMDV persistence. Interestingly, miR-1281was significantly down-regulated during both acute and persistent infection. Cattle that cleared infection resembled the baseline profile, adding support to applying serum miRNA profiling for identification of sub-clinically infected FMDV carriers. Significantly regulated miRNAs during acute or persistent infection were associated with cellular proliferation, apoptosis, modulation of the immune response, and lipid metabolism.
These findings suggest a role for non-coding regulatory RNAs in FMDV infection of cattle. Future studies will delineate the individual contributions of the reported miRNAs to FMDV replication, determine if this miRNA signature is applicable across all FMDV serotypes, and may facilitate development of novel diagnostic applications.
Additional file 1: Table S3. Cattle serum samples used for the miRNA profiling study. (DOCX 12 kb)12985_2017_743_MOESM1_ESM.docx
Additional file 2: Table S1. Predicted mRNA targets for indicated miRNAs. (DOCX 21 kb)12985_2017_743_MOESM2_ESM.docx
Additional file 3: Table S2. Predicted FMDV genomic targets for indicated miRNAs. (DOCX 15 kb)12985_2017_743_MOESM3_ESM.docx
Mason PW, Chinsangaram J, Moraes MP, Mayr GA, Grubman MJ. Engineering better vaccines for foot-and-mouth disease. Dev Biol (Basel). 2003;114:79–88.
Cejka D, Losert D, Wacheck V. Short interfering RNA (siRNA): tool or therapeutic? Clin Sci (Lond). 2006;110:47–58. CrossRef
Lee CH, Kim JH, Lee SW. The role of MicroRNA in pathogenesis and as markers of HCV chronic infection. Curr Drug Targets. 2016;17:1–10.
Wang C, Hann HW, Ye Z, Hann RS, Wan S, Ye X, Block PD, Li B, Myers RE, Wang X, et al. Prospective evidence of a circulating microRNA signature as a non-invasive marker of hepatocellular carcinoma in HBV patients. Oncotarget. 2016;9429:1–12.
Hussain M, Torres S, Schnettler E, Funk A, Grundhoff A, Pijlman GP, Khromykh AA, Asgari S. West Nile virus encodes a microRNA-like small RNA in the 3’ untranslated region which up-regulates GATA4 mRNA and facilitates virus replication in mosquito cells. Nucleic Acids Res. 2012;40:2210–23. PubMedCrossRef
Burrows R. Studies on the carrier state of cattle exposed to foot-and-mouth disease virus. J Hyg (Lond). 1966;64:81–90. CrossRef
Eschbaumer M, Stenfeldt C, Rekant SI, Pacheco JM, Hartwig EJ, Smoliga GR, Kenney MA, Golde WT, Rodriguez LL, Arzt J. Systemic immune response and virus persistence after foot-and-mouth disease virus infection of naive cattle and cattle vaccinated with a homologous adenovirus-vectored vaccine. BMC Vet Res. 2016;12:205. PubMedPubMedCentralCrossRef
Romao JM, Jin W, Dodson MV, Hausman GJ, Moore SS, Guan LL. MicroRNA regulation in mammalian adipogenesis. Exp Biol Med (Maywood). 2011;236:997–1004. CrossRef
Ding Z, Wang X, Khaidakov M, Liu S, Mehta JL. MicroRNA hsa-let-7g targets lectin-like oxidized low-density lipoprotein receptor-1 expression and inhibits apoptosis in human smooth muscle cells. Exp Biol Med (Maywood). 2012;237:1093–100. CrossRef
Maciejak A, Kiliszek M, Opolski G, Segiet A, Matlak K, Dobrzycki S, Tulacz D, Sygitowicz G, Burzynska B, Gora M. miR-22-5p revealed as a potential biomarker involved in the acute phase of myocardial infarction via profiling of circulating microRNAs. Mol Med Rep. 2016;14:2867–75. PubMed
Wan S, Ashraf U, Ye J, Duan X, Zohaib A, Wang W, Chen Z, Zhu B, Li Y, Chen H, Cao S. MicroRNA-22 negatively regulates poly(I:C)-triggered type I interferon and inflammatory cytokine production via targeting mitochondrial antiviral signaling protein (MAVS). Oncotarget. 2016;7:76667–83. PubMedPubMedCentral
Zhang S, Zhang D, Yi C, Wang Y, Wang H, Wang J. MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma. Oncol Rep. 2016;35:559–67. PubMed
Li JF, Dai XP, Zhang W, Sun SH, Zeng Y, Zhao GY, Kou ZH, Guo Y, Yu H, Du LY, et al. Upregulation of microRNA-146a by hepatitis B virus X protein contributes to hepatitis development by downregulating complement factor H. MBio. 2015;6(2):e02459-14.
Brauer-Hartmann D, Hartmann JU, Wurm AA, Gerloff D, Katzerke C, Verga Falzacappa MV, Pelicci PG, Muller-Tidow C, Tenen DG, Niederwieser D, Behre G. PML/RARalpha-regulated miR-181a/b cluster targets the tumor suppressor RASSF1A in acute promyelocytic leukemia. Cancer Res. 2015;75:3411–24. PubMedPubMedCentralCrossRef
Kaga H, Komatsuda A, Omokawa A, Ito M, Teshima K, Tagawa H, Sawada K, Wakui H. Downregulated expression of miR-155, miR-17, and miR-181b, and upregulated expression of activation-induced cytidine deaminase and interferon-alpha in PBMCs from patients with SLE. Mod Rheumatol. 2015;25:865–70. PubMedCrossRef
Cabrita MA, Vanzyl EJ, Hamill JD, Pan E, Marcellus KA, Tolls VJ, Alonzi RC, Pastic A, Rambo TM, Sayed H, McKay BC. A temperature sensitive variant of p53 drives p53-dependent MicroRNA expression without evidence of widespread post-transcriptional gene silencing. PLoS One. 2016;11:e0148529. PubMedPubMedCentralCrossRef
Tong AW, Fulgham P, Jay C, Chen P, Khalil I, Liu S, Senzer N, Eklund AC, Han J, Nemunaitis J. MicroRNA profile analysis of human prostate cancers. Cancer Gene Ther. 2009;16:206–16. PubMed
El-Guendy NM, Helwa R, El-Halawany MS, Abdel Rahman Ali S, Tantawy Aly M, Hasan Alieldin N, Fouad SA, Saeid H, Abdel-Wahab AH. The liver MicroRNA expression profiles associated with chronic hepatitis C virus (HCV) genotype-4 infection: a preliminary study. Hepat Mon. 2016;16:e33881. PubMedPubMedCentral
Pang X, Huang K, Zhang Q, Zhang Y, Niu J. miR-154 targeting ZEB2 in hepatocellular carcinoma functions as a potential tumor suppressor. Oncol Rep. 2015;34:3272–9. PubMed
Zhao D, Wang R, Fang J, Ji X, Li J, Chen X, Sun G, Wang Z, Liu W, Wang Y, et al. MiR-154 functions as a tumor suppressor in glioblastoma by targeting Wnt5a. Mol Neurobiol. 2016;1–8.
Zheng Y, Zhu C, Ma L, Shao P, Qin C, Li P, Cao Q, Ju X, Cheng G, Zhu Q, et al. miRNA-154-5p inhibits proliferation, migration and invasion by targeting E2F5 in prostate cancer cell lines. Urol Int. 2016;98(1):102–10.
Zhou H, Zhang M, Yuan H, Zheng W, Meng C, Zhao D. MicroRNA-154 functions as a tumor suppressor in osteosarcoma by targeting Wnt5a. Oncol Rep. 2016;35:1851–8. PubMed
Satoh M, Takahashi Y, Tabuchi T, Tamada M, Takahashi K, Itoh T, Morino Y, Nakamura M. Circulating Toll-like receptor 4-responsive microRNA panel in patients with coronary artery disease: results from prospective and randomized study of treatment with renin-angiotensin system blockade. Clin Sci (Lond). 2015;128:483–91. CrossRef
- Proof-of-concept study: profile of circulating microRNAs in Bovine serum harvested during acute and persistent FMDV infection
- BioMed Central
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