Background
Methods
Patients and sample collection
Small RNA transcriptome sequencing
NGS data analysis
Real time-quantitative PCR (RT-qPCR)
Results
Small non-coding RNA transcriptomes of Arab prostate cancer specimens
Library composition and mapping results
Sample | Total | Exon antisense | Exon sense | Intron antisense | Intron sense | miRNA | rRNA | Repeat | scRNA | snRNA | snoRNA | tRNA | Unann |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Q1N | 28,277,250a
100 | 2403 0.01 | 179469 0.63 | 6658 0.02 | 19,589 0.07 | 21,189,795 74.94 | 1,066,129 3.77 | 18,421 0.07 | 20,980 0.07 | 29,803 0.11 | 57,285 0.20 | 326,441 1.15 | 5,360,132 18.96 |
Q1T | 27,918,838 100 | 3281 0.01 | 189,423 0.68 | 9349 0.03 | 21,878 0.08 | 19,224,820 68.86 | 1,327,029 4.75 | 23,952 0.09 | 88,860 0.32 | 82,114 0.29 | 203,246 0.73 | 1,064,063 3.81 | 5,680,444 20.35 |
Q2N | 35,391,692 100 | 3470 0.01 | 298,030 0.84 | 26,105 0.07 | 80,756 0.23 | 15,998,067 45.2 | 5,369,926 15.17 | 76,891 0.22 | 196,764 0.56 | 199,951 0.56 | 221,878 0.63 | 8,445,871 23.86 | 4,472,358 12.64 |
Q2T | 33,773,903 100 | 10,013 0.03 | 524,796 1.55 | 25,779 0.08 | 164,968 0.49 | 18,912,932 56.00 | 4,493,690 13.31 | 158,765 0.47 | 106,115 0.31 | 855,596 2.53 | 690,244 2.04 | 767,289 2.27 | 7,062,748 20.91 |
Q3N | 35,735,732 100 | 1705 0.00 | 297,385 0.83 | 4644 0.01 | 24,114 0.07 | 23,955,712 67.04 | 1,974,819 5.53 | 35,437 0.10 | 151,635 0.42 | 71,211 0.20 | 131,070 0.37 | 1,937,351 5.42 | 7,150,228 20.01 |
Q3T | 36,095,235 100 | 4090 0.01 | 497,973 1.38 | 8652 0.02 | 82,083 0.23 | 19,275,478 53.40 | 3,251,981 9.01 | 104,960 0.29 | 189,016 0.52 | 281,679 0.78 | 516,959 1.43 | 5,106,698 14.15 | 6,774,339 18.77 |
Q4N | 45,235,749 100 | 2750 0.01 | 68,350 0.15 | 5387 0.01 | 14,524 0.03 | 34,012,084 75.19 | 1,382,848 3.06 | 29,433 0.07 | 71,732 0.16 | 30,671 0.07 | 71,326 0.16 | 982,107 2.17 | 8,564,307 18.93 |
Q4T | 32,598,915 100 | 2939 0.01 | 52,485 0.16 | 8446 0.03 | 18,961 0.06 | 24,364,229 74.74 | 690,166 2.12 | 45,388 0.14 | 43,589 0.13 | 32,698 0.10 | 106,106 0.33 | 484,692 1.49 | 6,749,107 20.70 |
Q5N | 34,197,138 100 | 15,613 0.05 | 1,110,918 3.25 | 4628 0.01 | 19,319 0.06 | 22,754,694 66.54 | 1,418,810 4.15 | 25,247 0.07 | 31,011 0.09 | 32,193 0.09 | 54,705 0.16 | 307,863 0.90 | 8,421,998 24.63 |
Q5T | 35,779,358 100 | 8384 0.02 | 297,959 0.83 | 8508 0.02 | 30,761 0.09 | 21,985,915 61.45 | 2,656,228 7.42 | 49,736 0.14 | 116,160 0.32 | 167,169 0.47 | 270,220 0.76 | 1,596,387 4.46 | 8,590,786 24.01 |
S1N | 32,055,346 100 | 6117 0.02 | 1,845,217 5.76 | 12,057 0.04 | 60,258 0.19 | 17,015,120 53.08 | 6,283,167 19.60 | 68,725 0.21 | 24,960 0.08 | 104,261 0.33 | 234,082 0.73 | 350,428 1.09 | 6,050,477 18.88 |
S1T | 41,112,010 100 | 17,603 0.04 | 3,936,180 9.57 | 44,451 0.11 | 251,699 0.61 | 8,314,806 20.22 | 20,102,292 48.90 | 344,943 0.84 | 69,247 0.17 | 428,991 1.04 | 986,772 2.40 | 672,186 1.64 | 5,940,943 14.45 |
S2N | 29,397,073 100 | 3898 0.01 | 897,203 3.05 | 7963 0.03 | 46,597 0.16 | 19,689,931 66.98 | 3,052,566 10.38 | 62,226 0.21 | 16,824 0.06 | 56,902 0.19 | 108,604 0.37 | 186,500 0.63 | 5,267,657 17.92 |
S2T | 26,137,643 100 | 13,962 0.05 | 295,974 1.13 | 16,398 0.06 | 95,037 0.36 | 10,893,729 41.68 | 7,311,047 27.97 | 129,995 0.50 | 61,762 0.24 | 212,641 0.81 | 494,207 1.89 | 912,770 3.49 | 5,699,007 21.80 |
S3N | 27,264,478 100 | 4766 0.02 | 226,353 0.83 | 15,660 0.06 | 39,350 0.14 | 18,702,182 68.60 | 2,369,035 8.69 | 92,587 0.34 | 30,752 0.11 | 57,087 0.21 | 127,926 0.47 | 230,624 0.85 | 5,367,833 19.69 |
S3T | 30,690,489 100 | 9707 0.03 | 2,599,251 8.47 | 17,755 0.06 | 99,710 0.32 | 16,952,101 55.24 | 4,516,427 14.72 | 116,291 0.38 | 44,387 0.14 | 183,781 0.60 | 415,388 1.35 | 349,767 1.14 | 5,385,487 17.55 |
S4N | 42,147,101 100 | 11,918 0.03 | 333,573 0.79 | 7982 0.02 | 43,044 0.10 | 31,240,514 74.12 | 3,129,257 7.42 | 52,155 0.12 | 30,886 0.07 | 64,665 0.15 | 129,612 0.31 | 273,707 0.65 | 6,829,490 16.20 |
S4T | 34,881,814 100 | 6136 0.02 | 71,254 0.20 | 11,995 0.03 | 24,355 0.07 | 25,685,322 73.64 | 1,061,270 3.04 | 61,568 0.18 | 51,806 0.15 | 27,056 0.08 | 59,345 0.17 | 476,327 1.37 | 7,345,173 21.06 |
S5N | 47,111,742 100 | 2764 0.01 | 495,709 1.05 | 11,953 0.03 | 31,017 0.07 | 35,338,827 75.01 | 1,482,687 3.15 | 61,971 0.13 | 70,137 0.15 | 56,524 0.12 | 127,766 0.27 | 462,461 0.98 | 8,969,539 19.04 |
S5T | 35,434,376 100 | 4534 0.01 | 254,597 0.72 | 10,168 0.03 | 39,678 0.11 | 24,840,095 70.10 | 3,051,937 8.61 | 65,451 0.18 | 45,425 0.13 | 41,078 0.12 | 107,746 0.30 | 978,442 2.76 | 5,994,715 16.92 |
Prostate cancer miRNA expression profiling
Up | Down | ||||
---|---|---|---|---|---|
miRNA | References | Note | miRNA | References | Note |
hsa-let-7a-5p | [8] | hsa-let-7e-5p | [8] | ||
hsa-miR-125a-5p | [8] | hsa-miR-107 | [8] | Up | |
hsa-miR-125b-5p | [8] | hsa-miR-130a-3p | [8] | ||
hsa-miR-141-3p | [8] | hsa-miR-143-3p | [8] | ||
hsa-miR-141-5p | [15] | hsa-miR-143-5p | |||
hsa-miR-148a-3p | [8] | hsa-miR-145-3p | |||
hsa-miR-148a-5p | [16] | hsa-miR-145-5p | [8] | ||
hsa-miR-148b-3p | [8] | hsa-miR-184 | [8] | ||
hsa-miR-148b-5p | [17] | hsa-miR-195-3p | |||
hsa-miR-151a-5p | [8] | hsa-miR-204-5p | [8] | ||
hsa-miR-15b-5p | [8] | hsa-miR-221-3p | [8] | ||
hsa-miR-17-5p | [8] | hsa-miR-221-5p | [8] | ||
hsa-miR-182-5p | [8] | hsa-miR-24-3p | [8] | ||
hsa-miR-183-5p | [8] | hsa-miR-30a-3p | [8] | ||
hsa-miR-191-5p | [8] | hsa-miR-320a | [18] | ||
hsa-miR-200c-3p | [8] | hsa-miR-320b | |||
hsa-miR-20b-5p | [8] | hsa-miR-328 | [8] | ||
hsa-miR-25-3p | [8] | hsa-miR-451a | [8] | ||
hsa-miR-363-3p | [8] | ||||
hsa-miR-375 | [8] | ||||
hsa-miR-423-3p | [8] | ||||
hsa-miR-425-5p | [8] | ||||
hsa-miR-484 | [8] | ||||
hsa-miR-671-3p | |||||
hsa-miR-92a-3p | [8] | ||||
hsa-miR-93-5p | [8] | ||||
hsa-miR-96-5p | [8] |
Identification of miRNAs targets
KEGG ID | KEGG term | Hypergeometric
P-value | Corrected
P-value (BF) | |
---|---|---|---|---|
hsa05200 | Pathways in cancer | 6.16E−08 | 1.23E−05 | |
hsa04110 | Cell cycle | 7.96E−08 | 1.58E−05 | |
hsa04144 | Endocytosis | 1.34E−07 | 2.67E−05 | |
Down | hsa04520 | Adherens junction | 2.55E−07 | 5.08E−05 |
hsa04310 | Wnt signaling pathway | 1.16E−06 | 2.31E−04 | |
hsa05220 | Chronic myeloid leukemia | 4.39E−06 | 8.73E−04 | |
hsa04114 | Oocyte meiosis | 4.90E−06 | 9.75E−04 | |
hsa05200 | Pathways in cancer | 2.47E−09 | 5.14E−07 | |
hsa05220 | Chronic myeloid leukemia | 4.54E−08 | 9.45E−06 | |
hsa04144 | Endocytosis | 3.80E−07 | 7.91E−05 | |
hsa05215 | Prostate cancer | 7.45E−07 | 1.55E−04 | |
Up | hsa04110 | Cell cycle | 7.71E−07 | 1.60E−04 |
hsa04115 | p53 signaling pathway | 2.31E−06 | 4.80E−04 | |
hsa04120 | Ubiquitin mediated proteolysis | 2.33E−06 | 4.84E−04 | |
hsa05210 | Colorectal cancer | 2.51E−06 | 5.22E−04 | |
hsa04520 | Adherens junction | 2.82E−06 | 5.87E−04 |
miRNA editing analysis
Patient | N/T | Count with base edit | Count without base edit | Count with base edit/total (%) |
---|---|---|---|---|
Q1 | N | 5,321,896 | 21,198,776 | 25.10 |
T | 7,787,197 | 19,220,573 | 28.83 | |
Q2 | N | 5,823,249 | 15,969,875 | 26.72 |
T | 7,467,449 | 18,853,718 | 28.37 | |
Q3 | N | 8,579,821 | 23,983,810 | 26.35 |
T | 9,210,644 | 19,298,828 | 32.30 | |
Q4 | N | 8,076,872 | 34,118,648 | 19.14 |
T | 7,532,111 | 24,404,950 | 23.58 | |
Q5 | N | 6,186,388 | 22,761,105 | 21.37 |
T | 8,750,023 | 22,044,971 | 28.41 | |
S1 | N | 10,050,073 | 17,020,307 | 37.13 |
T | 4,820,530 | 8,264,552 | 36.83 | |
S2 | N | 8,645,936 | 19,689,897 | 30.51 |
T | 5,213,543 | 10,899,980 | 32.36 | |
S3 | N | 6,020,810 | 18,710,729 | 24.34 |
T | 7,471,140 | 16,950,204 | 30.59 | |
S4 | N | 12,781,917 | 31,251,537 | 29.03 |
T | 6,021,975 | 25,742,476 | 18.96 | |
S5 | N | 11,084,722 | 35,390,547 | 23.85 |
T | 13,259,479 | 24,854,309 | 34.79 | |
Total | N | 82,571,684 | 240,095,231 | 25.59 |
T | 77,534,091 | 190,534,561 | 28.92 |
Patient | Non-tumor | Tumor | ||||
---|---|---|---|---|---|---|
Edited | Wildtype | Percentage | Edited | Wildtype | Percentage | |
Q1 | 240,370 | 87,935 | 73.22 | 557,205 | 44,596 | 92.59 |
Q2 | 446,002 | 64,943 | 87.29 | 854,634 | 32,478 | 96.34 |
Q3 | 212,470 | 9154 | 95.87 | 354,812 | 8077 | 97.77 |
Q4 | 151,335 | 8809 | 94.50 | 92,280 | 3395 | 96.45 |
Q5 | 116,611 | 17,170 | 87.17 | 142,264 | 7492 | 95.00 |
S1 | 535,538 | 27,674 | 95.09 | 197,120 | 11,975 | 94.27 |
S2 | 298,654 | 21,904 | 93.17 | 296,729 | 16,490 | 94.74 |
S3 | 174,168 | 20,140 | 89.64 | 203,879 | 15,356 | 93.00 |
S4 | 115,859 | 8238 | 93.36 | 83,231 | 4713 | 94.64 |
S5 | 235,026 | 12,658 | 94.89 | 194,898 | 14,228 | 93.20 |