Skip to main content
Erschienen in: Urolithiasis 6/2012

01.12.2012 | Original Paper

Protective effect of Flos carthami extract against ethylene glycol-induced urolithiasis in rats

verfasst von: Wu-Chou Lin, Ming-Tsung Lai, Huey-Yi Chen, Chien-Yi Ho, Kee-Ming Man, Jui-Lung Shen, Yuan-Ju Lee, Fuu-Jen Tsai, Yung-Hsiang Chen, Wen-Chi Chen

Erschienen in: Urolithiasis | Ausgabe 6/2012

Einloggen, um Zugang zu erhalten

Abstract

Flos carthami (FC), also known as Carthamus tinctorius, is a traditional Chinese herbal plant that has been prescribed since centuries for treating various symptoms related to blood circulation improvement. This study aimed to investigate the effects of FC on calcium oxalate (CaOx) formation in ethylene glycol (EG)-fed rats. A total of 50 male Sprague–Dawley rats were divided into the following 6 groups: group 1, as the normal control (n = 5); group 2 received gastric gavages of starch and 0.75% EG (placebo, n = 5) as a stone inducer; group 3 (n = 10) received EG and potassium citrate as positive controls; group 4 (n = 10) received 0.75% EG and 300 mg/day FC; group 5 (n = 10) was treated with EG and 600 mg/day FC; group 6 (n = 10) received with EG and 1,200 mg/day FC. For all experimental animals, 24-h urine and blood samples were analyzed at the beginning and end of the experiment. Kidney tissue was histopathologically examined using a polarized light microscope, and crystal deposits were evaluated by a semi-quantitative scoring method; these scores were significantly lower in the FC groups (600 and 1,200 mg/day) than in the placebo group. Thus, FC administration appeared to inhibit the deposition of CaOx crystal EG-fed rats. We, therefore, consider that FC may be effective for preventing stone disease, albeit with certain side effects, such as a bleeding tendency. Further clinical trials are needed for evaluating its benefits and possible side effects.
Literatur
1.
Zurück zum Zitat Lee YH, Huang WC, Tsai JY, Lu CM, Chen WC, Lee MH, Hsu HS, Huang JK, Chang LS (2002) Epidemiological studies on the prevalence of upper urinary calculi in Taiwan. Urol Int 68:172–177PubMedCrossRef Lee YH, Huang WC, Tsai JY, Lu CM, Chen WC, Lee MH, Hsu HS, Huang JK, Chang LS (2002) Epidemiological studies on the prevalence of upper urinary calculi in Taiwan. Urol Int 68:172–177PubMedCrossRef
2.
Zurück zum Zitat Bansal AD, Hui J, Goldfarb DS (2009) Asymptomatic nephrolithiasis detected by ultrasound. Clin J Am Soc Nephrol 4:680–684PubMedCrossRef Bansal AD, Hui J, Goldfarb DS (2009) Asymptomatic nephrolithiasis detected by ultrasound. Clin J Am Soc Nephrol 4:680–684PubMedCrossRef
3.
Zurück zum Zitat Sowers MR, Jannausch M, Wood C, Pope SK, Lachance LL, Peterson B (1998) Prevalence of renal stones in a population-based study with dietary calcium, oxalate, and medication exposures. Am J Epidemiol 147:914–920PubMedCrossRef Sowers MR, Jannausch M, Wood C, Pope SK, Lachance LL, Peterson B (1998) Prevalence of renal stones in a population-based study with dietary calcium, oxalate, and medication exposures. Am J Epidemiol 147:914–920PubMedCrossRef
4.
Zurück zum Zitat Goldfarb DS (2003) Increasing prevalence of kidney stones in the United States. Kidney Int 63:1951–1952PubMedCrossRef Goldfarb DS (2003) Increasing prevalence of kidney stones in the United States. Kidney Int 63:1951–1952PubMedCrossRef
5.
Zurück zum Zitat Brikowski TH, Lotan Y, Pearle MS (2008) Climate-related increase in the prevalence of urolithiasis in the United States. Proc Natl Acad Sci U S A 105:9841–9846PubMedCrossRef Brikowski TH, Lotan Y, Pearle MS (2008) Climate-related increase in the prevalence of urolithiasis in the United States. Proc Natl Acad Sci U S A 105:9841–9846PubMedCrossRef
6.
Zurück zum Zitat Lee YH, Huang WC, Tsai JY, Huang JK (1999) The efficacy of potassium citrate based medical prophylaxis for preventing upper urinary tract calculi: a midterm followup study. J Urol 161:1453–1457PubMedCrossRef Lee YH, Huang WC, Tsai JY, Huang JK (1999) The efficacy of potassium citrate based medical prophylaxis for preventing upper urinary tract calculi: a midterm followup study. J Urol 161:1453–1457PubMedCrossRef
7.
Zurück zum Zitat Tsai CH, Chen YC, Chen LD, Pan TC, Ho CY, Lai MT, Tsai FJ, Chen WC (2008) A traditional Chinese herbal antilithic formula, Wulingsan, effectively prevents the renal deposition of calcium oxalate crystal in ethylene glycol-fed rats. Urol Res 36:17–24PubMedCrossRef Tsai CH, Chen YC, Chen LD, Pan TC, Ho CY, Lai MT, Tsai FJ, Chen WC (2008) A traditional Chinese herbal antilithic formula, Wulingsan, effectively prevents the renal deposition of calcium oxalate crystal in ethylene glycol-fed rats. Urol Res 36:17–24PubMedCrossRef
8.
Zurück zum Zitat Tsai CH, Pan TC, Lai MT, Lee SC, Chen ML, Jheng JR, Chen WC (2009) Prophylaxis of experimentally induced calcium oxalate nephrolithiasis in rats by Zhulingtang, a traditional Chinese herbal formula. Urol Int 82:464–471PubMedCrossRef Tsai CH, Pan TC, Lai MT, Lee SC, Chen ML, Jheng JR, Chen WC (2009) Prophylaxis of experimentally induced calcium oxalate nephrolithiasis in rats by Zhulingtang, a traditional Chinese herbal formula. Urol Int 82:464–471PubMedCrossRef
9.
Zurück zum Zitat Yasui T, Fujita K, Sato M, Sugimoto M, Iguchi M, Nomura S, Kohri K (1999) The effect of takusha, a kampo medicine, on renal stone formation and osteopontin expression in a rat urolithiasis model. Urol Res 27:194–199PubMedCrossRef Yasui T, Fujita K, Sato M, Sugimoto M, Iguchi M, Nomura S, Kohri K (1999) The effect of takusha, a kampo medicine, on renal stone formation and osteopontin expression in a rat urolithiasis model. Urol Res 27:194–199PubMedCrossRef
10.
Zurück zum Zitat Atmani F, Khan SR (2000) Effects of an extract from Herniaria hirsuta on calcium oxalate crystallization in vitro. BJU Int 85:621–625PubMedCrossRef Atmani F, Khan SR (2000) Effects of an extract from Herniaria hirsuta on calcium oxalate crystallization in vitro. BJU Int 85:621–625PubMedCrossRef
11.
Zurück zum Zitat Freitas AM, Schor N, Boim MA (2002) The effect of Phyllanthus niruri on urinary inhibitors of calcium oxalate crystallization and other factors associated with renal stone formation. BJU Int 89:829–834PubMedCrossRef Freitas AM, Schor N, Boim MA (2002) The effect of Phyllanthus niruri on urinary inhibitors of calcium oxalate crystallization and other factors associated with renal stone formation. BJU Int 89:829–834PubMedCrossRef
12.
Zurück zum Zitat Liu QL, Sato S, Kishikawa T, Matsuzaki H, Yamanaka N (2001) Effectiveness of a traditional Chinese medicine, Wulingsan, in suppressing the development of nephrocalcinosis induced by a high phosphorus diet in young rats. Med Electron Microsc 34:103–114PubMedCrossRef Liu QL, Sato S, Kishikawa T, Matsuzaki H, Yamanaka N (2001) Effectiveness of a traditional Chinese medicine, Wulingsan, in suppressing the development of nephrocalcinosis induced by a high phosphorus diet in young rats. Med Electron Microsc 34:103–114PubMedCrossRef
13.
Zurück zum Zitat Koide T, Yamaguchi S, Utsunomiya M, Yoshioka T, Sugiyama K (1995) The inhibitory effect of kampou extracts on in vitro calcium oxalate crystallization and in vivo stone formation in an animal model. Int J Urol 2:81–86PubMedCrossRef Koide T, Yamaguchi S, Utsunomiya M, Yoshioka T, Sugiyama K (1995) The inhibitory effect of kampou extracts on in vitro calcium oxalate crystallization and in vivo stone formation in an animal model. Int J Urol 2:81–86PubMedCrossRef
14.
Zurück zum Zitat Al-Ghamdi SS, Al-Ghamdi AA, Shammah AA (2007) Inhibition of calcium oxalate nephrotoxicity with Cymbopogon schoenanthus (Al-Ethkher). Drug Metab Lett 1:241–244PubMedCrossRef Al-Ghamdi SS, Al-Ghamdi AA, Shammah AA (2007) Inhibition of calcium oxalate nephrotoxicity with Cymbopogon schoenanthus (Al-Ethkher). Drug Metab Lett 1:241–244PubMedCrossRef
15.
Zurück zum Zitat Chen YH, Liu HP, Chen HY, Tsai FJ, Chang CH, Lee YJ, Lin WY, Chen WC (2011) Ethylene glycol induces calcium oxalate crystal deposition in Malpighian tubules: a Drosophila model for nephrolithiasis/urolithiasis. Kidney Int 80:369–377PubMedCrossRef Chen YH, Liu HP, Chen HY, Tsai FJ, Chang CH, Lee YJ, Lin WY, Chen WC (2011) Ethylene glycol induces calcium oxalate crystal deposition in Malpighian tubules: a Drosophila model for nephrolithiasis/urolithiasis. Kidney Int 80:369–377PubMedCrossRef
16.
Zurück zum Zitat Knauf F, Preisig PA (2011) Drosophila: a fruitful model for calcium oxalate nephrolithiasis? Kidney Int 80:327–329PubMedCrossRef Knauf F, Preisig PA (2011) Drosophila: a fruitful model for calcium oxalate nephrolithiasis? Kidney Int 80:327–329PubMedCrossRef
17.
Zurück zum Zitat Li Y, Che Q (1998) Studies on chemical components of Carthamus tinctorius petals. Yao Xue Xue Bao 33:626–628PubMed Li Y, Che Q (1998) Studies on chemical components of Carthamus tinctorius petals. Yao Xue Xue Bao 33:626–628PubMed
18.
Zurück zum Zitat Sun M, Zhang JJ, Shan JZ, Zhang H, Jin CY, Xu S, Wang YL (2009) Clinical observation of Danhong Injection (herbal TCM product from Radix Salviae miltiorrhizae and Flos Carthami tinctorii) in the treatment of traumatic intracranial hematoma. Phytomedicine 16:683–689PubMedCrossRef Sun M, Zhang JJ, Shan JZ, Zhang H, Jin CY, Xu S, Wang YL (2009) Clinical observation of Danhong Injection (herbal TCM product from Radix Salviae miltiorrhizae and Flos Carthami tinctorii) in the treatment of traumatic intracranial hematoma. Phytomedicine 16:683–689PubMedCrossRef
19.
Zurück zum Zitat Lu Y, Hu YL, Kong XF, Wang DY (2008) Selection of component drug in activating blood flow and removing blood stasis of Chinese herbal medicinal formula for dairy cow mastitis by hemorheological method. J Ethnopharmacol 116:313–317PubMedCrossRef Lu Y, Hu YL, Kong XF, Wang DY (2008) Selection of component drug in activating blood flow and removing blood stasis of Chinese herbal medicinal formula for dairy cow mastitis by hemorheological method. J Ethnopharmacol 116:313–317PubMedCrossRef
20.
Zurück zum Zitat Zhang S, Xu C (1997) Progress in the clinical uses of flos Carthami. Zhongguo Zhong Yao Za Zhi 22:439–440, 442 Zhang S, Xu C (1997) Progress in the clinical uses of flos Carthami. Zhongguo Zhong Yao Za Zhi 22:439–440, 442
21.
Zurück zum Zitat Li HX, Han SY, Wang XW, Ma X, Zhang K, Wang L, Ma ZZ, Tu PF (2009) Effect of the carthamins yellow from Carthamus tinctorius L. on hemorheological disorders of blood stasis in rats. Food Chem Toxicol 47:1797–1802PubMedCrossRef Li HX, Han SY, Wang XW, Ma X, Zhang K, Wang L, Ma ZZ, Tu PF (2009) Effect of the carthamins yellow from Carthamus tinctorius L. on hemorheological disorders of blood stasis in rats. Food Chem Toxicol 47:1797–1802PubMedCrossRef
22.
Zurück zum Zitat Reagan-Shaw S, Nihal M, Ahmad N (2008) Dose translation from animal to human studies revisited. FASEB J 22:659–661PubMedCrossRef Reagan-Shaw S, Nihal M, Ahmad N (2008) Dose translation from animal to human studies revisited. FASEB J 22:659–661PubMedCrossRef
23.
Zurück zum Zitat Lee YH, Huang WC, Chiang H, Chen MT, Huang JK, Chang LS (1992) Determinant role of testosterone in the pathogenesis of urolithiasis in rats. J Urol 147:1134–1138PubMed Lee YH, Huang WC, Chiang H, Chen MT, Huang JK, Chang LS (1992) Determinant role of testosterone in the pathogenesis of urolithiasis in rats. J Urol 147:1134–1138PubMed
24.
Zurück zum Zitat McMartin K (2009) Are calcium oxalate crystals involved in the mechanism of acute renal failure in ethylene glycol poisoning? Clin Toxicol (Phila) 47:859–869CrossRef McMartin K (2009) Are calcium oxalate crystals involved in the mechanism of acute renal failure in ethylene glycol poisoning? Clin Toxicol (Phila) 47:859–869CrossRef
25.
Zurück zum Zitat Khan SR, Glenton PA, Byer KJ (2007) Dietary oxalate and calcium oxalate nephrolithiasis. J Urol 178:2191–2196PubMedCrossRef Khan SR, Glenton PA, Byer KJ (2007) Dietary oxalate and calcium oxalate nephrolithiasis. J Urol 178:2191–2196PubMedCrossRef
26.
Zurück zum Zitat Khan SR, Glenton PA, Byer KJ (2006) Modeling of hyperoxaluric calcium oxalate nephrolithiasis: experimental induction of hyperoxaluria by hydroxy-l-proline. Kidney Int 70:914–923PubMedCrossRef Khan SR, Glenton PA, Byer KJ (2006) Modeling of hyperoxaluric calcium oxalate nephrolithiasis: experimental induction of hyperoxaluria by hydroxy-l-proline. Kidney Int 70:914–923PubMedCrossRef
27.
Zurück zum Zitat Khan SR, Johnson JM, Peck AB, Cornelius JG, Glenton PA (2002) Expression of osteopontin in rat kidneys: induction during ethylene glycol induced calcium oxalate nephrolithiasis. J Urol 168:1173–1181PubMedCrossRef Khan SR, Johnson JM, Peck AB, Cornelius JG, Glenton PA (2002) Expression of osteopontin in rat kidneys: induction during ethylene glycol induced calcium oxalate nephrolithiasis. J Urol 168:1173–1181PubMedCrossRef
28.
Zurück zum Zitat Jammalamadaka D, Raissi S (2010) Ethylene glycol, methanol and isopropyl alcohol intoxication. Am J Med Sci 339:276–281PubMedCrossRef Jammalamadaka D, Raissi S (2010) Ethylene glycol, methanol and isopropyl alcohol intoxication. Am J Med Sci 339:276–281PubMedCrossRef
29.
Zurück zum Zitat Green ML, Hatch M, Freel RW (2005) Ethylene glycol induces hyperoxaluria without metabolic acidosis in rats. Am J Physiol Renal Physiol 289:F536–F543PubMedCrossRef Green ML, Hatch M, Freel RW (2005) Ethylene glycol induces hyperoxaluria without metabolic acidosis in rats. Am J Physiol Renal Physiol 289:F536–F543PubMedCrossRef
30.
Zurück zum Zitat Selvam R, Kalaiselvi P, Govindaraj A, Bala Murugan V, Sathish Kumar AS (2001) Effect of A. lanata leaf extract and Vediuppu chunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria. Pharmacol Res 43:89–93PubMedCrossRef Selvam R, Kalaiselvi P, Govindaraj A, Bala Murugan V, Sathish Kumar AS (2001) Effect of A. lanata leaf extract and Vediuppu chunnam on the urinary risk factors of calcium oxalate urolithiasis during experimental hyperoxaluria. Pharmacol Res 43:89–93PubMedCrossRef
31.
Zurück zum Zitat Araujo Viel T, Diogo Domingos C, da Silva Monteiro AP, Riggio Lima-Landman MT, Lapa AJ, Souccar C (1999) Evaluation of the antiurolithiatic activity of the extract of Costus spiralis Roscoe in rats. J Ethnopharmacol 66:193–198PubMedCrossRef Araujo Viel T, Diogo Domingos C, da Silva Monteiro AP, Riggio Lima-Landman MT, Lapa AJ, Souccar C (1999) Evaluation of the antiurolithiatic activity of the extract of Costus spiralis Roscoe in rats. J Ethnopharmacol 66:193–198PubMedCrossRef
32.
Zurück zum Zitat Gohel MD, Wong SP (2006) Chinese herbal medicines and their efficacy in treating renal stones. Urol Res 34:365–372PubMedCrossRef Gohel MD, Wong SP (2006) Chinese herbal medicines and their efficacy in treating renal stones. Urol Res 34:365–372PubMedCrossRef
33.
Zurück zum Zitat Cao ZG, Liu JH, Zhou SW, Wu W, Yin CP, Wu JZ (2004) The effects of the active constituents of Alisma orientalis on renal stone formation and bikunin expression in rat urolithiasis model. Zhonghua Yi Xue Za Zhi 84:1276–1279PubMed Cao ZG, Liu JH, Zhou SW, Wu W, Yin CP, Wu JZ (2004) The effects of the active constituents of Alisma orientalis on renal stone formation and bikunin expression in rat urolithiasis model. Zhonghua Yi Xue Za Zhi 84:1276–1279PubMed
34.
Zurück zum Zitat Yoshimura K, Miyake O, Okuyama A, Yoshioka T, Honda M, Yamaguchi S, Koide T (1998) Effect of chorei-to and gorei-san on calcium oxalate crystallization in human urine. Hinyokika Kiyo 44:13–16PubMed Yoshimura K, Miyake O, Okuyama A, Yoshioka T, Honda M, Yamaguchi S, Koide T (1998) Effect of chorei-to and gorei-san on calcium oxalate crystallization in human urine. Hinyokika Kiyo 44:13–16PubMed
35.
Zurück zum Zitat Miyaoka R, Monga M (2009) Use of traditional Chinese medicine in the management of urinary stone disease. Int Braz J Urol 35:396–405PubMedCrossRef Miyaoka R, Monga M (2009) Use of traditional Chinese medicine in the management of urinary stone disease. Int Braz J Urol 35:396–405PubMedCrossRef
36.
Zurück zum Zitat Cao ZG, Liu JH, Radman AM, Wu JZ, Ying CP, Zhou SW (2003) An experimental study of effect of different extracts of Alisma orientalis on urinary calcium oxalate stones formation in rats. Zhongguo Zhong Yao Za Zhi 28:1072–1075PubMed Cao ZG, Liu JH, Radman AM, Wu JZ, Ying CP, Zhou SW (2003) An experimental study of effect of different extracts of Alisma orientalis on urinary calcium oxalate stones formation in rats. Zhongguo Zhong Yao Za Zhi 28:1072–1075PubMed
37.
Zurück zum Zitat Butterweck V, Khan SR (2009) Herbal medicines in the management of urolithiasis: alternative or complementary? Planta Med 75:1095–1103PubMedCrossRef Butterweck V, Khan SR (2009) Herbal medicines in the management of urolithiasis: alternative or complementary? Planta Med 75:1095–1103PubMedCrossRef
38.
Zurück zum Zitat Atmani F (2003) Medical management of urolithiasis, what opportunity for phytotherapy? Front Biosci 8:s507–s514PubMedCrossRef Atmani F (2003) Medical management of urolithiasis, what opportunity for phytotherapy? Front Biosci 8:s507–s514PubMedCrossRef
Metadaten
Titel
Protective effect of Flos carthami extract against ethylene glycol-induced urolithiasis in rats
verfasst von
Wu-Chou Lin
Ming-Tsung Lai
Huey-Yi Chen
Chien-Yi Ho
Kee-Ming Man
Jui-Lung Shen
Yuan-Ju Lee
Fuu-Jen Tsai
Yung-Hsiang Chen
Wen-Chi Chen
Publikationsdatum
01.12.2012
Verlag
Springer-Verlag
Erschienen in
Urolithiasis / Ausgabe 6/2012
Print ISSN: 2194-7228
Elektronische ISSN: 2194-7236
DOI
https://doi.org/10.1007/s00240-012-0472-4

Weitere Artikel der Ausgabe 6/2012

Urolithiasis 6/2012 Zur Ausgabe

Update Urologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.