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Erschienen in: Endocrine 3/2015

01.04.2015 | Original Article

Protective effect of Schisandrae chinensis oil on pancreatic β-cells in diabetic rats

verfasst von: Liping An, Yingping Wang, Chunmei Wang, Meizhen Fan, Xiao Han, Guangyu Xu, Guangxin Yuan, Hongyu Li, Yu Sheng, Manli Wang, Jingbo Sun, Jinzhuo Zhan, Hui Sun, Na Li, Fuxiang Ding, Peige Du

Erschienen in: Endocrine | Ausgabe 3/2015

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Abstract

Islet cell dysfunction in type 2 diabetes is primarily attributed to increased apoptosis of pancreatic β-Cells. The aim of the present study was to investigate the effects of Schisandrae chinensis oil on pancreatic β-Cells in type 2 diabetes mellitus rats and the associated molecular mechanisms of action. Wistar rats were randomly divided into diabetic rats and control rats, diabetic rats treated with Schisandrae chinensis oil (1 mg/kg), and control rats treated with Schisandrae chinensis oil. The serum fasting blood glucose, insulin, total cholesterol, and triglyceride levels along with MDA content, SOD and CAT activities in pancreatic tissues were measured. TUNEL was used to observe the apoptosis of rat pancreatic cells. Western blot was used to determine specific protein expression. The results showed that the oil significantly decreased fasting blood glucose, total cholesterol, triglyceride levels as well as the pancreatic MDA, but increased SOD and CAT activities. The protein expression of Bcl-2, PDX-1, GLUT-2, and GCK but not caspase 3 was significantly enhanced in the oil-treated rats compared with diabetic rats. However, Bax content was not significantly different between the control and DM groups. Schisandra chinensis oil improves pancreatic β-cell function by enhancing antioxidant potential of the pancreas, upregulating the expression of anti-apoptotic genes, increasing expression of glucose metabolism, and delaying islet cell apoptosis.
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Metadaten
Titel
Protective effect of Schisandrae chinensis oil on pancreatic β-cells in diabetic rats
verfasst von
Liping An
Yingping Wang
Chunmei Wang
Meizhen Fan
Xiao Han
Guangyu Xu
Guangxin Yuan
Hongyu Li
Yu Sheng
Manli Wang
Jingbo Sun
Jinzhuo Zhan
Hui Sun
Na Li
Fuxiang Ding
Peige Du
Publikationsdatum
01.04.2015
Verlag
Springer US
Erschienen in
Endocrine / Ausgabe 3/2015
Print ISSN: 1355-008X
Elektronische ISSN: 1559-0100
DOI
https://doi.org/10.1007/s12020-014-0375-y

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