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Graefe's Archive for Clinical and Experimental Ophthalmology

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Open Access 24.07.2020 | Basic Science

Protective effect of the extremolytes ectoine and hydroxyectoine in a porcine organ culture

verfasst von: Teresa Tsai, Ana M. Mueller-Buehl, Yathavan Satgunarajah, Sandra Kuehn, H. Burkhard Dick, Stephanie C. Joachim

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 10/2020

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Abstract

Purpose

Hypoxic damage to the retina is a relevant component of neurodegenerative pathologies such as glaucoma or retinal ischemia. In porcine retina organ cultures, hypoxic damage can be induced by applying cobalt chloride (CoCl₂). The aim of our study was to investigate possible neuroprotective effects of the extremolytes ectoine and hydroxyectoine in this hypoxia-damaged retina model.

Methods

To simulate hypoxia, porcine retina organ cultures were damaged with 300 μM CoCl₂ for 48 h starting on day 1 (n = 8–9/group). In order to investigate the possible neuroprotective effects of ectoine and hydroxyectoine, 0.5 mM of each extremolyte was added to the culture at the same time as the stressor and for the same duration. On day 8, the retina organ cultures were taken for (immuno)-histochemical examinations. Retinal ganglion cells (RGCs), macroglia, and apoptotic and hypoxic cells were detected with appropriate markers followed by cell counts and group comparisons.

Results

Treatment with ectoine resulted in RGC protection (p < 0.05) and reduced rate of apoptosis (p < 0.001) in hypoxia-treated retina organ cultures. However, the macroglia area and the amount of hypoxic, HIF-1α⁺ cells were unaffected by the ectoine treatment (p = 0.99). Treatment with hydroxyectoine also protected RGCs (p < 0.01) by inhibiting apoptosis (p < 0.001). In addition, the number of hypoxic, HIF-1α⁺ cells could be significantly reduced by treatment with hydroxyectoine (p < 0.05). The macroglia area on the other hand was unchanged after CoCl₂ and treatment with hydroxyectoine.

Conclusion

Both extremolytes had a protective effect on CoCl₂-induced hypoxia in the porcine retina organ culture. Regarding the reduction of hypoxic stress, hydroxyectoine appears to be more effective. Thus, both extremolytes represent an interesting potential new therapeutic approach for patients with ocular diseases in which hypoxic processes play a significant role.

Cohen LH, Noell WK (1965) Relationships between visual function and metabolism. In: Graymore CN (ed) Biochemistry of the Retina. Academic press Inc, Orlando, pp 36–50

Wangsa-Wirawan ND, Linsenmeier RA (2003) Retinal oxygen: fundamental and clinical aspects. Arch Ophthalmol 121(4):547–557. https://doi.org/10.1001/archopht.121.4.547CrossRefPubMed

Kaur C, Foulds WS, Ling EA (2008) Hypoxia-ischemia and retinal ganglion cell damage. Clin Ophthalmol 2(4):879–889. https://doi.org/10.2147/opth.s3361CrossRefPubMedPubMedCentral

Linsenmeier RA, Braun RD, McRipley MA, Padnick LB, Ahmed J, Hatchell DL, McLeod DS, Lutty GA (1998) Retinal hypoxia in long-term diabetic cats. Invest Ophthalmol Vis Sci 39(9):1647–1657PubMed

Harris A, Arend O, Danis RP, Evans D, Wolf S, Martin BJ (1996) Hyperoxia improves contrast sensitivity in early diabetic retinopathy. Br J Ophthalmol 80(3):209–213. https://doi.org/10.1136/bjo.80.3.209CrossRefPubMedPubMedCentral

Flammer J (1994) The vascular concept of glaucoma. Surv Ophthalmol 38(Suppl):S3–S6. https://doi.org/10.1016/0039-6257(94)90041-8CrossRefPubMed

Tielsch JM, Katz J, Sommer A, Quigley HA, Javitt JC (1995) Hypertension, perfusion pressure, and primary open-angle glaucoma. A population-based assessment. Arch Ophthalmol 113(2):216–221. https://doi.org/10.1001/archopht.1995.01100020100038CrossRefPubMed

Chung HS, Harris A, Evans DW, Kagemann L, Garzozi HJ, Martin B (1999) Vascular aspects in the pathophysiology of glaucomatous optic neuropathy. Surv Ophthalmol 43(Suppl 1):S43–S50. https://doi.org/10.1016/s0039-6257(99)00050-8CrossRefPubMed

EGS (2017) European Glaucoma society terminology and guidelines for Glaucoma, 4th edition - Chapter2: classification and terminology. Br J Ophthalmol 101(5):73–127. https://doi.org/10.1136/bjophthalmol-2016-EGSguideline.002CrossRef

10.

Quigley HA, Broman AT (2006) The number of people with glaucoma worldwide in 2010 and 2020. Br J Ophthalmol 90(3):262–267. https://doi.org/10.1136/bjo.2005.081224CrossRefPubMedPubMedCentral

11.

Tham YC, Li X, Wong TY, Quigley HA, Aung T, Cheng CY (2014) Global prevalence of glaucoma and projections of glaucoma burden through 2040: a systematic review and meta-analysis. Ophthalmology 121(11):2081–2090. https://doi.org/10.1016/j.ophtha.2014.05.013CrossRefPubMed

12.

McDonnell F, Irnaten M, Clark AF, O'Brien CJ, Wallace DM (2016) Hypoxia-induced changes in DNA methylation Alter RASAL1 and TGFbeta1 expression in human trabecular meshwork cells. PLoS One 11(4):e0153354. https://doi.org/10.1371/journal.pone.0153354CrossRefPubMedPubMedCentral

13.

Tezel G, Wax MB (2004) Hypoxia-inducible factor 1alpha in the glaucomatous retina and optic nerve head. Arch Ophthalmol 122(9):1348–1356. https://doi.org/10.1001/archopht.122.9.1348CrossRefPubMed

14.

Semenza GL, Agani F, Iyer N, Jiang BH, Leung S, Wiener C, Yu A (1998) Hypoxia-inducible factor 1: from molecular biology to cardiopulmonary physiology. Chest 114(1 Suppl):40S–45S. https://doi.org/10.1378/chest.114.1_supplement.40sCrossRefPubMed

15.

Huang LE, Gu J, Schau M, Bunn HF (1998) Regulation of hypoxia-inducible factor 1alpha is mediated by an O2-dependent degradation domain via the ubiquitin-proteasome pathway. Proc Natl Acad Sci U S A 95(14):7987–7992. https://doi.org/10.1073/pnas.95.14.7987CrossRefPubMedPubMedCentral

16.

Hellwig-Burgel T, Stiehl DP, Wagner AE, Metzen E, Jelkmann W (2005) Review: hypoxia-inducible factor-1 (HIF-1): a novel transcription factor in immune reactions. J Interferon Cytokine Res. 25(6):297–310. https://doi.org/10.1089/jir.2005.25.297CrossRefPubMed

17.

Lopez-Hernandez B, Cena V, Posadas I (2015) The endoplasmic reticulum stress and the HIF-1 signalling pathways are involved in the neuronal damage caused by chemical hypoxia. Br J Pharmacol 172(11):2838–2851. https://doi.org/10.1111/bph.13095CrossRefPubMedPubMedCentral

18.

Ergorul C, Ray A, Huang W, Wang DY, Ben Y, Cantuti-Castelvetri I, Grosskreutz CL (2010) Hypoxia inducible factor-1alpha (HIF-1alpha) and some HIF-1 target genes are elevated in experimental glaucoma. J Mol Neurosci : MN 42(2):183–191. https://doi.org/10.1007/s12031-010-9343-zCrossRefPubMed

19.

Reszec J, Zalewska R, Bernaczyk P, Chyczewski L (2012) HIF-1 expression in retinal ganglion cells and optic nerve axons in glaucoma. Folia Histochem Cytobiol 50(3):456–459. https://doi.org/10.5603/19757CrossRefPubMed

20.

Yamada K (2013) Cobalt: its role in health and disease. Met Ions Life Sci 13:295–320. https://doi.org/10.1007/978-94-007-7500-8_9CrossRefPubMed

21.

Caltana L, Merelli A, Lazarowski A, Brusco A (2009) Neuronal and glial alterations due to focal cortical hypoxia induced by direct cobalt chloride (CoCl2) brain injection. Neurotox Res 15(4):348–358. https://doi.org/10.1007/s12640-009-9038-9CrossRefPubMed

22.

Grasselli F, Basini G, Bussolati S, Bianco F (2005) Cobalt chloride, a hypoxia-mimicking agent, modulates redox status and functional parameters of cultured swine granulosa cells. Reprod Fertil Dev 17(7):715–720CrossRef

23.

Cheng Z, Yao W, Zheng J, Ding W, Wang Y, Zhang T, Zhu L, Zhou F (2019) A derivative of betulinic acid protects human retinal pigment epithelial (RPE) cells from cobalt chloride-induced acute hypoxic stress. Exp Eye Res 180:92–101. https://doi.org/10.1016/j.exer.2018.12.011CrossRefPubMed

24.

Zimmerman MA, Biggers CD, Li PA (2018) Rapamycin treatment increases hippocampal cell viability in an mTOR-independent manner during exposure to hypoxia mimetic, cobalt chloride. BMC Neurosci 19(1):82. https://doi.org/10.1186/s12868-018-0482-4CrossRefPubMedPubMedCentral

25.

del Olmo-Aguado S, Nunez-Alvarez C, Ji D, Manso AG, Osborne NN (2013) RTP801 immunoreactivity in retinal ganglion cells and its down-regulation in cultured cells protect them from light and cobalt chloride. Brain Res Bull 98:132–144. https://doi.org/10.1016/j.brainresbull.2013.08.002CrossRefPubMed

26.

Kuehn S, Hurst J, Rensinghoff F, Tsai T, Grauthoff S, Satgunarajah Y, Dick HB, Schnichels S, Joachim SC (2017) Degenerative effects of cobalt-chloride treatment on neurons and microglia in a porcine retina organ culture model. Exp Eye Res 155:107–120. https://doi.org/10.1016/j.exer.2017.01.003CrossRefPubMed

27.

Rothschild LJ, Mancinelli RL (2001) Life in extreme environments. Nature 409(6823):1092–1101. https://doi.org/10.1038/35059215CrossRefPubMed

28.

Lippert K, Galinski EA (1992) Enzyme stabilization by ectoine-type compatible solutes; protection against heating, freezing and drying. Appl Microbiol Biotechnol 37:61. https://doi.org/10.1007/BF00174204CrossRef

29.

Arakawa T, Timasheff SN (1985) The stabilization of proteins by osmolytes. Biophys J 47(3):411–414. https://doi.org/10.1016/S0006-3495(85)83932-1CrossRefPubMedPubMedCentral

30.

Malin G, Iakobashvili R, Lapidot A (1999) Effect of tetrahydropyrimidine derivatives on protein-nucleic acids interaction. Type II restriction endonucleases as a model system. J Biol Chem 274(11):6920–6929. https://doi.org/10.1074/jbc.274.11.6920CrossRefPubMed

31.

Galinski EA, Pfeiffer HP, Truper HG (1985) 1,4,5,6-Tetrahydro-2-methyl-4-pyrimidinecarboxylic acid. A novel cyclic amino acid from halophilic phototrophic bacteria of the genus Ectothiorhodospira. Eur J Biochem 149(1):135–139CrossRef

32.

Inbar L, Lapidot A (1988) The structure and biosynthesis of new tetrahydropyrimidine derivatives in actinomycin D producer Streptomyces parvulus. Use of 13C- and 15N-labeled L-glutamate and 13C and 15N NMR spectroscopy. J Biol Chem 263(31):16014–16022CrossRef

33.

Zaccai G, Bagyan I, Combet J, Cuello GJ, Deme B, Fichou Y, Gallat FX, Galvan Josa VM, von Gronau S, Haertlein M, Martel A, Moulin M, Neumann M, Weik M, Oesterhelt D (2016) Neutrons describe ectoine effects on water H-bonding and hydration around a soluble protein and a cell membrane. Sci Rep 6:31434. https://doi.org/10.1038/srep31434CrossRefPubMedPubMedCentral

34.

Buenger J, Driller H (2004) Ectoin: an effective natural substance to prevent UVA-induced premature photoaging. Skin Pharmacol Physiol 17(5):232–237. https://doi.org/10.1159/000080216CrossRefPubMed

35.

Graf R, Anzali S, Buenger J, Pfluecker F, Driller H (2008) The multifunctional role of ectoine as a natural cell protectant. Clin Dermatol 26(4):326–333. https://doi.org/10.1016/j.clindermatol.2008.01.002CrossRefPubMed

36.

Marini A, Reinelt K, Krutmann J, Bilstein A (2014) Ectoine-containing cream in the treatment of mild to moderate atopic dermatitis: a randomised, comparator-controlled, intra-individual double-blind, multi-center trial. Skin Pharmacol Physiol 27(2):57–65. https://doi.org/10.1159/000351381CrossRefPubMed

37.

Werkhauser N, Bilstein A, Sonnemann U (2014) Treatment of allergic rhinitis with ectoine containing nasal spray and eye drops in comparison with azelastine containing nasal spray and eye drops or with cromoglycic acid containing nasal spray. J Allergy 2014:176597. https://doi.org/10.1155/2014/176597CrossRef

38.

Sonnemann U, Moller M, Bilstein A (2014) Noninterventional open-label trial investigating the efficacy and safety of ectoine containing nasal spray in comparison with beclomethasone nasal spray in patients with allergic rhinitis. J Allergy 2014:297203. https://doi.org/10.1155/2014/297203CrossRef

39.

Eichel A, Bilstein A, Werkhauser N, Mosges R (2014) Meta-analysis of the efficacy of ectoine nasal spray in patients with allergic rhinoconjunctivitis. J Allergy 2014:292545. https://doi.org/10.1155/2014/292545CrossRef

40.

Muller D, Lindemann T, Shah-Hosseini K, Scherner O, Knop M, Bilstein A, Mosges R (2016) Efficacy and tolerability of an ectoine mouth and throat spray compared with those of saline lozenges in the treatment of acute pharyngitis and/or laryngitis: a prospective, controlled, observational clinical trial. Eur Arch Oto-Rhino L 273(9):2591–2597. https://doi.org/10.1007/s00405-016-4060-zCrossRef

41.

Sydlik U, Gallitz I, Albrecht C, Abel J, Krutmann J, Unfried K (2009) The compatible solute ectoine protects against nanoparticle-induced neutrophilic lung inflammation. Am J Respir Crit Care Med 180(1):29–35. https://doi.org/10.1164/rccm.200812-1911OCCrossRefPubMed

42.

Sydlik U, Peuschel H, Paunel-Gorgulu A, Keymel S, Kramer U, Weissenberg A, Kroker M, Seghrouchni S, Heiss C, Windolf J, Bilstein A, Kelm M, Krutmann J, Unfried K (2013) Recovery of neutrophil apoptosis by ectoine: a new strategy against lung inflammation. Eur Respir J 41(2):433–442. https://doi.org/10.1183/09031936.00132211CrossRefPubMed

43.

Unfried K, Kroker M, Autengruber A, Gotic M, Sydlik U (2014) The compatible solute ectoine reduces the exacerbating effect of environmental model particles on the immune response of the airways. J Allergy 2014:708458. https://doi.org/10.1155/2014/708458CrossRef

44.

Eichel A, Wittig J, Shah-Hosseini K, Mosges R (2013) A prospective, controlled study of SNS01 (ectoine nasal spray) compared to BNO-101 (phytotherapeutic dragees) in patients with acute rhinosinusitis. Curr Med Res Opin 29(7):739–746. https://doi.org/10.1185/03007995.2013.800474CrossRefPubMed

45.

Hurst J, Kuehn S, Jashari A, Tsai T, Bartz-Schmidt KU, Schnichels S, Joachim SC (2017) A novel porcine ex vivo retina culture model for oxidative stress induced by H(2)O(2). Altern Lab Anim : ATLA 45(1):11–25CrossRef

46.

Kuehn S, Hurst J, Jashari A, Ahrens K, Tsai T, Wunderlich IM, Dick HB, Joachim SC, Schnichels S (2016) The novel induction of retinal ganglion cell apoptosis in porcine organ culture by NMDA - an opportunity for the replacement of animals in experiments. Altern Lab Anim : ATLA 44(6):557–568CrossRef

47.

Maliha AM, Kuehn S, Hurst J, Herms F, Fehr M, Bartz-Schmidt KU, Dick HB, Joachim SC, Schnichels S (2019) Diminished apoptosis in hypoxic porcine retina explant cultures through hypothermia. Sci Rep 9(1):4898. https://doi.org/10.1038/s41598-019-41113-4CrossRefPubMedPubMedCentral

48.

Horstmann L, Schmid H, Heinen AP, Kurschus FC, Dick HB, Joachim SC (2013) Inflammatory demyelination induces glia alterations and ganglion cell loss in the retina of an experimental autoimmune encephalomyelitis model. J Neuroinflammation 10:120. https://doi.org/10.1186/1742-2094-10-120CrossRefPubMedPubMedCentral

49.

Reinehr S, Kuehn S, Casola C, Koch D, Stute G, Grotegut P, Dick HB, Joachim SC (2018) HSP27 immunization reinforces AII amacrine cell and synapse damage induced by S100 in an autoimmune glaucoma model. Cell Tissue Res 371(2):237–249. https://doi.org/10.1007/s00441-017-2710-0CrossRefPubMed

50.

Casola C, Reinehr S, Kuehn S, Stute G, Spiess BM, Dick HB, Joachim SC (2016) Specific inner retinal layer cell damage in an autoimmune Glaucoma model is induced by GDNF with or without HSP27. Invest Ophthalmol Vis Sci 57(8):3626–3639. https://doi.org/10.1167/iovs.15-18999R2CrossRefPubMed

51.

Oren A (1999) Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev : MMBR 63(2):334–348CrossRef

52.

Antón J (2011) Compatible Solute. In: Gargaud M, Amils R, Quintanilla JC et al (eds) Encyclopedia of astrobiology. Springer, Berlin Heidelberg, pp 351–352. https://doi.org/10.1007/978-3-642-11274-4_336CrossRef

53.

Arora A, Ha C, Park CB (2004) Inhibition of insulin amyloid formation by small stress molecules. FEBS Lett 564(1–2):121–125. https://doi.org/10.1016/S0014-5793(04)00326-6CrossRefPubMed

54.

Furusho K, Yoshizawa T, Shoji S (2005) Ectoine alters subcellular localization of inclusions and reduces apoptotic cell death induced by the truncated Machado-Joseph disease gene product with an expanded polyglutamine stretch. Neurobiol Dis 20(1):170–178. https://doi.org/10.1016/j.nbd.2005.02.011CrossRefPubMed

55.

Kanapathipillai M, Ku SH, Girigoswami K, Park CB (2008) Small stress molecules inhibit aggregation and neurotoxicity of prion peptide 106-126. Biochem Biophys Res Commun 365(4):808–813. https://doi.org/10.1016/j.bbrc.2007.11.074CrossRefPubMed

56.

Kanapathipillai M, Lentzen G, Sierks M, Park CB (2005) Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer's beta-amyloid. FEBS Lett 579(21):4775–4780. https://doi.org/10.1016/j.febslet.2005.07.057CrossRefPubMed

57.

Caldas T, Demont-Caulet N, Ghazi A, Richarme G (1999) Thermoprotection by glycine betaine and choline. Microbiology 145(Pt 9):2543–2548. https://doi.org/10.1099/00221287-145-9-2543CrossRefPubMed

58.

Kim YG, Lim HH, Lee SH, Shin MS, Kim CJ, Yang HJ (2015) Betaine inhibits vascularization via suppression of Akt in the retinas of streptozotocin-induced hyperglycemic rats. Mol Med Rep 12(2):1639–1644. https://doi.org/10.3892/mmr.2015.3613CrossRefPubMedPubMedCentral

59.

Park SW, Jun HO, Kwon E, Yun JW, Kim JH, Park YJ, Kang BC, Kim JH (2017) Antiangiogenic effect of betaine on pathologic retinal neovascularization via suppression of reactive oxygen species mediated vascular endothelial growth factor signaling. Vasc Pharmacol 90:19–26. https://doi.org/10.1016/j.vph.2016.07.007CrossRef

60.

Hseu YC, Chen XZ, Vudhya Gowrisankar Y, Yen HR, Chuang JY, Yang HL (2020) The skin-whitening effects of Ectoine via the suppression of alpha-MSH-stimulated Melanogenesis and the activation of antioxidant Nrf2 pathways in UVA-irradiated keratinocytes. Antioxidants (Basel) 9(1). https://doi.org/10.3390/antiox9010063

61.

Kolamunne RT, Dias IH, Vernallis AB, Grant MM, Griffiths HR (2013) Nrf2 activation supports cell survival during hypoxia and hypoxia/reoxygenation in cardiomyoblasts; the roles of reactive oxygen and nitrogen species. Redox Biol 1:418–426. https://doi.org/10.1016/j.redox.2013.08.002CrossRefPubMedPubMedCentral

62.

Sheng L, Lu B, Chen H, Du Y, Chen C, Cai W, Yang Y, Tian X, Huang Z, Chi W, Lin S, Yan G, Yin W (2019) Marine-steroid derivative 5alpha-Androst-3beta, 5alpha, 6beta-triol protects retinal ganglion cells from ischemia(−)reperfusion injury by activating Nrf2 pathway. Mar Drugs 17(5). https://doi.org/10.3390/md17050267

63.

Nakazawa T, Tachi S, Aikawa E, Ihnuma M (1993) Formation of the myelinated nerve fiber layer in the chicken retina. Glia 8(2):114–121. https://doi.org/10.1002/glia.440080207CrossRefPubMed

64.

Taylor L, Arner K, Ghosh F (2016) N-methyl-N-nitrosourea-induced neuronal cell death in a large animal model of retinal degeneration in vitro. Exp Eye Res 148:55–64. https://doi.org/10.1016/j.exer.2016.05.023CrossRefPubMed

65.

Knapp S, Ladenstein R, Galinski EA (1999) Extrinsic protein stabilization by the naturally occurring osmolytes beta-hydroxyectoine and betaine. Extremophiles 3(3):191–198CrossRef

66.

Zhang L, Wang Y, Zhang C, Wang Y, Zhu D, Wang C, Nagata S (2006) Supplementation effect of ectoine on thermostability of phytase. J Biosci Bioeng 102(6):560–563. https://doi.org/10.1263/jbb.102.560CrossRefPubMed

67.

Harishchandra RK, Wulff S, Lentzen G, Neuhaus T, Galla HJ (2010) The effect of compatible solute ectoines on the structural organization of lipid monolayer and bilayer membranes. Biophys Chem 150(1–3):37–46. https://doi.org/10.1016/j.bpc.2010.02.007CrossRefPubMed

68.

Smiatek J, Harishchandra RK, Rubner O, Galla HJ, Heuer A (2012) Properties of compatible solutes in aqueous solution. Biophys Chem 160(1):62–68. https://doi.org/10.1016/j.bpc.2011.09.007CrossRefPubMed

69.

Borges N, Ramos A, Raven ND, Sharp RJ, Santos H (2002) Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes. Extremophiles 6(3):209–216. https://doi.org/10.1007/s007920100236CrossRefPubMed

70.

Louis P, Trtiper HG, Galinski EA (1994) Survival of Escherichia coli during drying and storage in the presence of compatible solutes. Appl Microbiol Biotechnol 41:684–688CrossRef

71.

Schnoor M, Voss P, Cullen P, Boking T, Galla HJ, Galinski EA, Lorkowski S (2004) Characterization of the synthetic compatible solute homoectoine as a potent PCR enhancer. Biochem Biophys Res Commun 322(3):867–872. https://doi.org/10.1016/j.bbrc.2004.07.200CrossRefPubMed

72.

Bursy J, Kuhlmann AU, Pittelkow M, Hartmann H, Jebbar M, Pierik AJ, Bremer E (2008) Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses. Appl Environ Microbiol 74(23):7286–7296. https://doi.org/10.1128/AEM.00768-08CrossRefPubMedPubMedCentral

73.

Herzog M, Dwivedi M, Kumar Harishchandra R, Bilstein A, Galla HJ, Winter R (2019) Effect of ectoine, hydroxyectoine and beta-hydroxybutyrate on the temperature and pressure stability of phospholipid bilayer membranes of different complexity. Colloids Surf B: Biointerfaces 178:404–411. https://doi.org/10.1016/j.colsurfb.2019.03.026CrossRefPubMed

Titel: Protective effect of the extremolytes ectoine and hydroxyectoine in a porcine organ culture
verfasst von: Teresa Tsai
Ana M. Mueller-Buehl
Yathavan Satgunarajah
Sandra Kuehn
H. Burkhard Dick
Stephanie C. Joachim
Publikationsdatum: 24.07.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 10/2020
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-020-04854-x

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Protective effect of the extremolytes ectoine and hydroxyectoine in a porcine organ culture

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