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Cardiovascular Toxicology

Erschienen in:

10.07.2020

Protective Effects of Spermidine and Melatonin on Deltamethrin-Induced Cardiotoxicity and Neurotoxicity in Zebrafish

verfasst von: Xingyu Liu, Qian Gao, Zeyang Feng, Yaqiu Tang, Xin Zhao, Dongyan Chen, Xizeng Feng

Erschienen in: Cardiovascular Toxicology | Ausgabe 1/2021

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Abstract

Increased application of the pyrethroid insecticide deltamethrin has adverse effects on the cardiac system and neurobehavior on the non-target organisms, which has raised the public’s attention. Because of spermidine and melatonin considered to have cardioprotective and neuroprotective characteristics, zebrafish were utilized as the model organism to explore the protective effects of spermidine and melatonin against deltamethrin-induced toxicity. We tested the neurobehavior of zebrafish larvae through a rest/wake behavior assay, and evaluated the levels of the expression of Scn5lab, gata4, nkx2.5, hcrt, hcrtr, and aanat2 by qRT-PCR. Besides that cmlc2 was evaluated by whole-mount in situ hybridization. Results have shown that compared with control group, 0.025 mg/L deltamethrin could significantly disturb the cardiac development, downregulating the expression of Scn5lab and transcriptional factors gata4 and nkx2.5, disturbing cardiac looping, resulting in defects in cardiac morphology and function. Moreover, deltamethrin could alter the expression levels of rest/wake genes and cause hyperactivity in zebrafish larvae. Besides, compared with deltamethrin group, the exogenous 0.01 mg/L spermidine and 0.232 mg/L melatonin could significantly rescue the adverse effects of deltamethrin on the cardiac system and neurobehavior in zebrafish. This indicated that spermidine and melatonin have neuroprotective and cardioprotective effects against deltamethrin-induced adverse effects in zebrafish.

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Elliott, M., Farnham, A. W., Janes, N. F., Needham, P. H., & Pulman, D. A. (1974). Synthetic insecticide with a new order of activity. Nature, 248, 710–711.PubMedCrossRef

Soderlund, D. M. (2012). Molecular mechanisms of pyrethroid insecticide neurotoxicity: Recent advances. Archives of Toxicology, 86, 165–181.PubMedCrossRef

Bouwman, H., Sereda, B., & Meinhardt, H. M. (2006). Simultaneous presence of DDT and pyrethroid residues in human breast milk from a malaria endemic area in South Africa. Environmental Pollution, 144, 902–917.PubMedCrossRef

Whyatt, R. M., Garfinkel, R., Hoepner, L. A., Holmes, D., Borjas, M., Williams, M. K., et al. (2007). Within- and between-home variability in indoor-air insecticide levels during pregnancy among an inner-city cohort from New York City. Environmental Health Perspectives, 115, 383–389.PubMedCrossRef

Naeher, L. P., Tulve, N. S., Egeghy, P. P., Barr, D. B., Adetona, O., Fortmann, R. C., et al. (2010). Organophosphorus and pyrethroid insecticide urinary metabolite concentrations in young children living in a southeastern United States city. Science of the Total Environment, 408, 1145–1153.CrossRefPubMed

Richardson, J. R., Taylor, M. M., Shalat, S. L., Guillot, T. S., Caudle, W. M., Hossain, M. M., et al. (2015). Developmental pesticide exposure reproduces features of attention deficit hyperactivity disorder. The FASEB Journal, 29, 1960–1972.PubMedPubMedCentralCrossRef

Dhivya Vadhana, M. S., Siva Arumugam, S., Carloni, M., Nasuti, C., & Gabbianelli, R. (2013). Early life permethrin treatment leads to long-term cardiotoxicity. Chemosphere, 93, 1029–1034.PubMedCrossRef

Luo, H., Masika, J., Guan, X., Nie, L., Ao, D., Qi, Y., et al. (2019). Long term perinatal deltamethrin exposure alters electrophysiological properties of embryonic ventricular cardiomyocyte. Current Medical Science, 39, 21–27.PubMedCrossRef

Widmark, J., Sundstrom, G., Ocampo Daza, D., & Larhammar, D. (2011). Differential evolution of voltage-gated sodium channels in tetrapods and teleost fishes. Molecular Biology and Evolution, 28, 859–871.PubMedCrossRef

10.

Moreno, J. D., & Clancy, C. E. (2012). Pathophysiology of the cardiac late Na current and its potential as a drug target. Journal of Molecular and Cellular Cardiology, 52, 608–619.PubMedCrossRef

11.

Mahboob, S., Niazi, F., AlGhanim, K., Sultana, S., Al-Misned, F., & Ahmed, Z. (2015). Health risks associated with pesticide residues in water, sediments and the muscle tissues of Catla catla at Head Balloki on the River Ravi. Environmental Monitoring and Assessment, 187, 81.PubMedCrossRef

12.

Kylin, H. B. A. H. (2014). Malaria control insecticide residues in breast milk: The need to consider infant health risks. Environmental Health Perspectives, 117(10), 1477–1480.

13.

Li, M., Liu, X., & Feng, X. (2019). Cardiovascular toxicity and anxiety-like behavior induced by deltamethrin in zebrafish (Danio rerio) larvae. Chemosphere, 219, 155–164.PubMedCrossRef

14.

Raina, A., & Jänne, J. (1975). Physiology of the natural polyamines putrescine, spermidine and spermine. Medicine and Biology, 53, 121–147.

15.

Eisenberg, T., Knauer, H., Schauer, A., Büttner, S., Ruckenstuhl, C., Carmona-Gutierrez, D., et al. (2009). Induction of autophagy by spermidine promotes longevity. Nature Cell Biology, 11, 1305–1314.PubMedCrossRef

16.

Eisenberg, T., Abdellatif, M., Schroeder, S., Primessnig, U., Stekovic, S., Pendl, T., et al. (2016). Cardioprotection and lifespan extension by the natural polyamine spermidine. Nature Medicine, 22, 1428–1438.PubMedPubMedCentralCrossRef

17.

Sharma, S., Kumar, P., & Deshmukh, R. (2018). Neuroprotective potential of spermidine against rotenone induced Parkinson's disease in rats. Neurochemistry International, 116, 104–111.PubMedCrossRef

18.

Karbownik, M., & Reiter, R. J. (2000). Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation. Proceedings of the Society for Experimental Biology and Medicine, 225, 9–22.PubMedCrossRef

19.

Iggena, D., Winter, Y., & Steiner, B. (2017). Melatonin restores hippocampal neural precursor cell proliferation and prevents cognitive deficits induced by jet lag simulation in adult mice. Journal of Pineal Research, 62, e12397.CrossRef

20.

Calvo, J. R., Gonzalez-Yanes, C., & Maldonado, M. D. (2013). The role of melatonin in the cells of the innate immunity: A review. Journal of Pineal Research, 55, 103–120.PubMedCrossRef

21.

Su, S.-C., Hsieh, M.-J., Yang, W.-E., Chung, W.-H., Reiter, R. J., & Yang, S.-F. (2017). Cancer metastasis: Mechanisms of inhibition by melatonin. Journal of Pineal Research, 62, e12370.CrossRef

22.

Pandi-Perumal, S. R., BaHammam, A. S., Ojike, N. I., Akinseye, O. A., Kendzerska, T., Buttoo, K., et al. (2017). Melatonin and human cardiovascular disease. Journal of Cardiovascular Pharmacology and Therapeutics, 22, 122–132.PubMedCrossRef

23.

Brown, D. R., Samsa, L. A., Qian, L., & Liu, J. (2016). Advances in the study of heart development and disease using zebrafish. Journal of Cardiovascular Development and Disease, 3(2), 13.PubMedPubMedCentralCrossRef

24.

Renier, C., Faraco, J. H., Bourgin, P., Motley, T., Bonaventure, P., Rosa, F., et al. (2007). Genomic and functional conservation of sedative-hypnotic targets in the zebrafish. Pharmacogenetics and Genomics, 17, 237–253.PubMedCrossRef

25.

Zhu, J.-J., Xu, Y.-Q., He, J.-H., Yu, H.-P., Huang, C.-J., Gao, J.-M., et al. (2014). Human cardiotoxic drugs delivered by soaking and microinjection induce cardiovascular toxicity in zebrafish: Zebrafish model for assessing drug-induced cardiovascular toxicity. Journal of Applied Toxicology, 34, 139–148.PubMedCrossRef

26.

Haverinen, J., & Vornanen, M. (2016). Deltamethrin is toxic to the fish (crucian carp, Carassius carassius) heart. Pesticide Biochemistry and Physiology, 129, 36–42.PubMedCrossRef

27.

Zhang, S., Xu, J., Kuang, X., Li, S., Li, X., Chen, D., et al. (2017). Biological impacts of glyphosate on morphology, embryo biomechanics and larval behavior in zebrafish (Danio rerio). Chemosphere, 181, 270–280.PubMedCrossRef

28.

Haverinen, J., Hassinen, M., Korajoki, H., & Vornanen, M. (2018). Cardiac voltage-gated sodium channel expression and electrophysiological characterization of the sodium current in the zebrafish (Danio rerio) ventricle. Progress in Biophysics and Molecular Biology, 138, 59–68.PubMedCrossRef

29.

Hoage, T., Ding, Y., & Xu, X. (2012). Quantifying cardiac functions in embryonic and adult zebrafish. In X. Peng & M. Antonyak (Eds.), Cardiovascular development: Methods and protocols, methods in molecular biology (pp. 11–20). Totowa, NJ: Humana Press.CrossRef

30.

Poss, K. D., Keating, M. T., & Nechiporuk, A. (2003). Tales of regeneration in zebrafish. Developmental Dynamics, 226, 202–210.PubMedCrossRef

31.

Chopra, S. S., Stroud, D. M., Watanabe, H., Bennett, J. S., Burns, C. G., Wells, K. S., et al. (2010). Voltage-gated sodium channels are required for heart development in zebrafish. Circulation Research, 106, 1342–1350.PubMedPubMedCentralCrossRef

32.

Chueh, T. C., Hsu, L. S., Kao, C. M., Hsu, T. W., Liao, H. Y., Wang, K. Y., et al. (2017). Transcriptome analysis of zebrafish embryos exposed to deltamethrin. Environmental Toxicology, 32, 1548–1557.PubMedCrossRef

33.

Gorge, G., & Nagel, R. (1990). Toxicity of lindane, atrazine, and deltamethrin to early life stages of zebrafish (Brachydanio rerio). Ecotoxicology and Environmental Safety, 20, 246–255.PubMedCrossRef

34.

Novak, A. E., Taylor, A. D., Pineda, R. H., Lasda, E. L., Wright, M. A., & Ribera, A. B. (2006). Embryonic and larval expression of zebrafish voltage-gated sodium channel α-subunit genes. Developmental Dynamics, 235, 1962–1973.PubMedCrossRef

35.

Papadatos, G. A., Wallerstein, P. M. R., Head, C. E. G., Ratcliff, R., Brady, P. A., Benndorf, K., et al. (2002). Slowed conduction and ventricular tachycardia after targeted disruption of the cardiac sodium channel gene Scn5a. Proceedings of the National academy of Sciences of the United States of America, 99, 6210–6215.PubMedPubMedCentralCrossRef

36.

Balci, M. M., & Akdemir, R. (2011). NKX2.5 mutations and congenital heart disease: Is it a marker of cardiac anomalies? International Journal of Cardiology, 147, e44–45.PubMedCrossRef

37.

Zhou, P., He, A., & Pu, W. T. (2012). Regulation of GATA4 transcriptional activity in cardiovascular development and disease. Current Topics in Developmental Biology, 100, 143–169.PubMedCrossRef

38.

Yeung, H.-M., Hung, M.-W., Lau, C.-F., & Fung, M.-L. (2015). Cardioprotective effects of melatonin against myocardial injuries induced by chronic intermittent hypoxia in rats. Journal of Pineal Research, 58, 12–25.PubMedCrossRef

39.

Zheng, J., Yu, Y., Feng, W., Li, J., Liu, J., Zhang, C., et al. (2019). Influence of nanomolar deltamethrin on the hallmarks of primary cultured cortical neuronal network and the role of ryanodine receptors. Environmental Health Perspectives, 127, 67003.PubMedCrossRef

40.

Zhdanova, I. V., Wang, S. Y., Leclair, O. U., & Danilova, N. P. (2001). Melatonin promotes sleep-like state in zebrafish. Brain Research, 903, 263–268.PubMedCrossRef

41.

Appelbaum, L., Wang, G. X., Maro, G. S., Mori, R., Tovin, A., Marin, W., et al. (2009). Sleep–wake regulation and hypocretin–melatonin interaction in zebrafish. Proceedings of the National Academy of Sciences of the United States of America, 106, 21942–21947.PubMedPubMedCentralCrossRef

42.

Appelbaum, L., Vallone, D., Anzulovich, A., Ziv, L., Tom, M., Foulkes, N. S., et al. (2006). Zebrafish arylalkylamine-N-acetyltransferase genes—Targets for regulation of the circadian clock. Journal of Molecular Endocrinology, 36, 337–347.PubMedCrossRef

Titel: Protective Effects of Spermidine and Melatonin on Deltamethrin-Induced Cardiotoxicity and Neurotoxicity in Zebrafish
verfasst von: Xingyu Liu
Qian Gao
Zeyang Feng
Yaqiu Tang
Xin Zhao
Dongyan Chen
Xizeng Feng
Publikationsdatum: 10.07.2020
Verlag: Springer US
Erschienen in: Cardiovascular Toxicology / Ausgabe 1/2021
Print ISSN: 1530-7905
Elektronische ISSN: 1559-0259
DOI: https://doi.org/10.1007/s12012-020-09591-5

Springer Medizin

Abstract

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