The online version of this article (doi:10.1007/s00394-016-1255-4) contains supplementary material, which is available to authorized users.
Eric A. P. Steegers and Trudy Voortman have contributed equally to this article.
Intra-uterine exposure to protein may affect body composition and may increase the prevalence of childhood adiposity. Therefore, we examined whether protein intake during pregnancy is associated with offspring body composition at the age of 6 years and whether associations differ for animal protein and vegetable protein.
We included 2694 Dutch mother–child pairs participating in a prospective population-based cohort in Rotterdam, the Netherlands. Energy-adjusted protein was measured in pregnancy using a food-frequency questionnaire and analyzed in quartiles. At a mean age of 6.1 ± 0.4 years, we measured children’s body mass index, and fat-free mass index and fat mass index using dual-energy X-ray absorptiometry. Outcomes were standardized for age and sex. BMI was used to classify children’s overweight status.
After adjustment for sociodemographic and lifestyle factors, a higher maternal protein intake was associated with a higher children’s fat-free mass index [difference 0.14 standard deviation (95 % CI 0.03, 0.25) for highest vs. lowest quartile of protein intake], but not with children’s fat mass index or body mass index. Comparable associations were found for animal protein and vegetable protein. Maternal protein intake was not associated with children’s overweight.
This study suggests that higher protein intake during pregnancy is associated with a higher fat-free mass in children at the age of 6 years, but not with their fat mass. Our results do not suggest specific recommendations regarding maternal protein intake during pregnancy to prevent overweight in the offspring.
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World Health Organization (WHO) Obesity and overweight. http://www.who.int/mediacentre/factsheets/fs311/en/#
Bradfield JP, Taal HR, Timpson NJ, Scherag A, Lecoeur C, Warrington NM, Hypponen E, Holst C, Valcarcel B, Thiering E, Salem RM, Schumacher FR, Cousminer DL, Sleiman PM, Zhao J, Berkowitz RI, Vimaleswaran KS, Jarick I, Pennell CE, Evans DM, St Pourcain B, Berry DJ, Mook-Kanamori DO, Hofman A, Rivadeneira F, Uitterlinden AG, van Duijn CM, van der Valk RJ, de Jongste JC, Postma DS, Boomsma DI, Gauderman WJ, Hassanein MT, Lindgren CM, Magi R, Boreham CA, Neville CE, Moreno LA, Elliott P, Pouta A, Hartikainen AL, Li M, Raitakari O, Lehtimaki T, Eriksson JG, Palotie A, Dallongeville J, Das S, Deloukas P, McMahon G, Ring SM, Kemp JP, Buxton JL, Blakemore AI, Bustamante M, Guxens M, Hirschhorn JN, Gillman MW, Kreiner-Moller E, Bisgaard H, Gilliland FD, Heinrich J, Wheeler E, Barroso I, O’Rahilly S, Meirhaeghe A, Sorensen TI, Power C, Palmer LJ, Hinney A, Widen E, Farooqi IS, McCarthy MI, Froguel P, Meyre D, Hebebrand J, Jarvelin MR, Jaddoe VW, Smith GD, Hakonarson H, Grant SF, Early Growth Genetics C (2012) A genome-wide association meta-analysis identifies new childhood obesity loci. Nat Genet 44(5):526–531. doi: 10.1038/ng.2247 CrossRef
Muhlhausler BS, Ong ZY (2011) The fetal origins of obesity: early origins of altered food intake. Endocr Metab Immune Disord Drug Targets 11(3):189–197 CrossRef
Maslova E, Rytter D, Bech BH, Henriksen TB, Rasmussen MA, Olsen SF, Halldorsson TI (2014) Maternal protein intake during pregnancy and offspring overweight 20 y later. Am J Clin Nutr. doi: 10.3945/ajcn.113.082222
Kruithof CJ, Kooijman MN, van Duijn CM, Franco OH, de Jongste JC, Klaver CC, Mackenbach JP, Moll HA, Raat H, Rings EH, Rivadeneira F, Steegers EA, Tiemeier H, Uitterlinden AG, Verhulst FC, Wolvius EB, Hofman A, Jaddoe VW (2014) The Generation R Study: Biobank update 2015. Eur J Epidemiol 29(12):911–927. doi: 10.1007/s10654-014-9980-6 CrossRef
Statistics Netherlands (2004) Allochtonen in Nederland 2004. Statistics Netherlands, Voorburg/Heerlen
Klipstein-Grobusch K, den Breeijen JH, Goldbohm RA, Geleijnse JM, Hofman A, Grobbee DE, Witteman JC (1998) Dietary assessment in the elderly: validation of a semiquantitative food frequency questionnaire. Eur J Clin Nutr 52(8):588–596 CrossRef
Netherlands-Nutrition-Centre (2006) NEVO: Dutch food composition database 2006. Hoontetijl, The Hague
Cole TJ, Bellizzi MC, Flegal KM, Dietz WH (2000) Establishing a standard definition for child overweight and obesity worldwide: international survey. BMJ 320(7244):1240–1243 CrossRef
Willett WC, Howe GR, Kushi LH (1997) Adjustment for total energy intake in epidemiologic studies. Am J Clin Nutr 65(4 Suppl):1220S–1228S (discussion 1229S–1231S)
Hu FB, Stampfer MJ, Rimm E, Ascherio A, Rosner BA, Spiegelman D, Willett WC (1999) Dietary fat and coronary heart disease: a comparison of approaches for adjusting for total energy intake and modeling repeated dietary measurements. Am J Epidemiol 149(6):531–540 CrossRef
Rubin DB (1987) Multiple imputation for nonresponse in surveys. Wiley, London CrossRef
Rehfeldt C, Lefaucheur L, Block J, Stabenow B, Pfuhl R, Otten W, Metges CC, Kalbe C (2012) Limited and excess protein intake of pregnant gilts differently affects body composition and cellularity of skeletal muscle and subcutaneous adipose tissue of newborn and weanling piglets. Eur J Nutr 51(2):151–165. doi: 10.1007/s00394-011-0201-8 CrossRef
Svendsen OL, Haarbo J, Hassager C, Christiansen C (1993) Accuracy of measurements of body composition by dual-energy x-ray absorptiometry in vivo. Am J Clin Nutr 57(5):605–608
Kipnis V, Subar AF, Midthune D, Freedman LS, Ballard-Barbash R, Troiano RP, Bingham S, Schoeller DA, Schatzkin A, Carroll RJ (2003) Structure of dietary measurement error: results of the OPEN biomarker study. Am J Epidemiol 158(1):14–21 (discussion 22–16) CrossRef
Greenland S (1988) Variance estimation for epidemiologic effect estimates under misclassification. Stat Med 7(7):745–757 CrossRef
Sterne JA, White IR, Carlin JB, Spratt M, Royston P, Kenward MG, Wood AM, Carpenter JR (2009) Multiple imputation for missing data in epidemiological and clinical research: potential and pitfalls. BMJ 338:b2393 CrossRef
- Protein intake during pregnancy and offspring body composition at 6 years: the Generation R Study
Myrte J. Tielemans
Eric A. P. Steegers
Vincent W. V. Jaddoe
Oscar H. Franco
Jessica C. Kiefte-de Jong
- Springer Berlin Heidelberg