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Clinical and Translational Oncology

Erschienen in:

01.02.2014 | Research Article

Proteomic analysis of pleural effusion from lung adenocarcinoma patients by shotgun strategy

verfasst von: Shu-hong Sheng, Hui-li Zhu

Erschienen in: Clinical and Translational Oncology | Ausgabe 2/2014

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Abstract

Purpose

To construct a protein catalogue of malignant pleural effusion from lung adenocarcinoma patients and to screen the potential candidates of biomarkers for diagnostic value in human lung adenocarcinoma.

Method

Five malignant pleural effusion samples of lung adenocarcinoma patients were collected from January 2009 to September. A composite sample was analyzed using shotgun strategy. Pleural effusion samples were separated by means of SDS-PAGE. Proteomic analysis was performed by 1D-LC–MS/MS, and then the proteins were identified using SEQUEST software and protein database search.

Results

Among 230 unique proteins, 123 proteins were identified with higher confidence levels (at least two unique peptide sequences matched). Most of these proteins have been reported in plasma. However, there are 7 proteins, including JUP protein, suprabasin, annexin A2, transforming growth factor-beta-induced protein ig-h3 (βig-h3), V-set and immunoglobulin domain-containing protein 4 precursor, ifapsoriasin 2 and actin, cytoplasmic 1 have not been reported in serum.

Conclusions

Seven proteins may represent potential candidates of biomarkers. Annexin A2 is of special interest since it may play a role in the regulation of intercellular adhesion and cell proliferation.

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Titel: Proteomic analysis of pleural effusion from lung adenocarcinoma patients by shotgun strategy
verfasst von: Shu-hong Sheng
Hui-li Zhu
Publikationsdatum: 01.02.2014
Verlag: Springer Milan
Erschienen in: Clinical and Translational Oncology / Ausgabe 2/2014
Print ISSN: 1699-048X
Elektronische ISSN: 1699-3055
DOI: https://doi.org/10.1007/s12094-013-1054-9

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Proteomic analysis of pleural effusion from lung adenocarcinoma patients by shotgun strategy

Abstract

Purpose

Method

Results

Conclusions

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ICI-Therapie in der Schwangerschaft wird gut toleriert

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Method

Results

Conclusions

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