Proteomics identifies hypothermia induced adiponectin protects corneal endothelial cells via AMPK mediated autophagy in phacoemulsification
- 08.06.2024
- Basic Science
Erschienen in:
- Verfasst von
- Yanyi Chen
- Kewei Li
- Rongxi Huang
- Liang Xiong
- Ruonan Li
- Lu Jiang
- Yan Xun
- Wenjuan Wan
- Ke Hu
Abstract
Aim
To explore the molecular mechanism underlying the protective effect of hypothermic perfusion on the corneal endothelium during phacoemulsification.
Methods
Phacoemulsification was performed on New Zealand white rabbits. Perfusate at different temperatures was used during the operation, and the aqueous humor was collected for proteomic sequencing after the operation. Corneal endothelial cell injury was simulated by a corneal endothelial cell oxygen–glucose deprivation/reoxygenation (OGD/R) model in vitro. Flow cytometry and evaluation of fluorescent LC3B puncta were used to detect apoptosis and autophagy, and western blotting was used to detect protein expression.
Results
A total of 381 differentially expressed proteins were identified between the two groups. In vitro, 4 ℃ hypothermia significantly reduced apoptosis and promoted autophagy. Apoptosis increased after autophagy was inhibited by 3-Methyladenine (3-MA). Furthermore, adiponectin (ADIPOQ) knockdown inhibited phospho-AMPK and blocked the protective effect of hypothermia on corneal endothelial cells.
Conclusions
We investigated the differential expression of proteins between the hypothermia group and normothermia group by proteomics. Moreover, hypothermia-induced ADIPOQ can reduce apoptosis by promoting AMPK-mediated autophagy.
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- Titel
- Proteomics identifies hypothermia induced adiponectin protects corneal endothelial cells via AMPK mediated autophagy in phacoemulsification
- Verfasst von
-
Yanyi Chen
Kewei Li
Rongxi Huang
Liang Xiong
Ruonan Li
Lu Jiang
Yan Xun
Wenjuan Wan
Ke Hu
- Publikationsdatum
- 08.06.2024
- Verlag
- Springer Berlin Heidelberg
- Erschienen in
-
Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 10/2024
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X - DOI
- https://doi.org/10.1007/s00417-024-06542-6
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