nach oben

Inflammopharmacology

Erschienen in:

10.02.2018 | Original Article

Prunus cerasoides fruit extract ameliorates inflammatory stress by modulation of iNOS pathway and Th1/Th2 immune homeostasis in activated murine macrophages and lymphocytes

verfasst von: Anamika Sharma, Robin Joshi, Shiv Kumar, Rohit Sharma, Rajneesh, Yogendra Padwad, Mahesh Gupta

Erschienen in: Inflammopharmacology | Ausgabe 6/2018

Einloggen, um Zugang zu erhalten

Abstract

The present investigation assessed the potential of Prunus cerasoides fruit extract (PCFE) in alleviation of inflammatory stress in response to lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-stimulated murine peritoneal macrophages as well as in concanavalin A (Con A)-activated splenic lymphocytes. We observed a strong inhibition in production of nitric oxide (NO), reactive oxygen species (ROS), inflammatory cytokines (TNF-α/IL-6/IL-1β), inducible nitric oxide synthase (iNOS), and NF-kB in macrophages treated with PCFE. Splenic lymphocytes treated with PCFE also showed a reduction in Con-A-induced cell proliferation and numbers of CD3⁺CD4⁺ T cells. Furthermore, PCFE treatment to Con A-stimulated lymphocytes decreased the production of inflammatory cytokines (TNF-α/IL-6/IL-1β) with a concomitant increase in IL-10 suggesting its possible role in alleviation of inflammation-driven Th1/Th2 immune imbalance. PCFE appeared to influence innate immune response even at lower concentrations (25 and 50 µg/ml), while such effects were more pronounced in lymphocytes only at higher concentrations (100 and 200 µg/ml). UPLC–ESI–MS of PCFE revealed the presence of major bioactive phenolics including catechin, naringin as well as ascorbic acid which could have contributed in above findings. Overall, it is indicative that P. cerasoides fruit could be a valuable source for the development of anti-inflammatory functional foods and nutraceuticals.

Graphical Abstract

Arulselvan P, Fard MT, Tan WS, Gothai S, Fakurazi S (2016) Role of antioxidants and natural products in inflammation. Oxid Med Cell Longev 2016:1–15CrossRef

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O (2012) Oxidative stress and antioxidant defense. World Allergy Organ J. 5:9–19CrossRefPubMedPubMedCentral

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMed

Chandrasekaran CV, Kannan Sundarajan JRE, Gururaja GM, Mundkinajeddu D, Agarwal A (2013) Immune-stimulatory and anti-inflammatory activities of Curcuma longa extract and its polysaccharide fraction. Pharmacogn Res. 5:71–79CrossRef

Chen Y, Luo G, Yuan J, Wang Y, Yang X, Wang X, Li G, Liu Z, Zhong N (2014) Vitamin C mitigates oxidative stress and tumor necrosis factor-alpha in severe community-acquired pneumonia and LPS-induced macrophages. Mediat Inflamm 2014:1–12

Cho HJ, Lee KW, Park JHY (2013) Erucin exerts anti-inflammatory properties inmurine macrophages and mouse skin: possible mediation through the inhibition of NFκB signaling. Int J Mol Sci 14:20564–20577CrossRefPubMedPubMedCentral

Fan FY, Sang LX, Jiang M (2017) Catechins and their therapeutic benefits to inflammatory bowel disease. Molecules 22:484–513CrossRefPubMedCentral

Gao X, Deeb D, Liu Y, Gautam S, Dulchavsky SA, Gautam SC (2009) Immunomodulatory activity of xanthohumol: inhibition of T cell proliferation, cell-mediated cytotoxicity and Th1 cytokine production through suppression of NF-κB. Immunopharmacol Immunotoxicol 31:477–484CrossRefPubMedPubMedCentral

Gil MI, Tomás-Barberán FA, Hess-Pierce B, Kader AA (2002) Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. J Agric Food Chem 50:4976–4982CrossRefPubMed

Gil M, Kim YK, Hong SB, Lee KJ (2016) Naringin Decreases TNF-α and HMGB1 Release from LPS-stimulated macrophages and improves survival in a CLP-induced sepsis mice. PLoS One 11:164186–164202

Guan S, Fang B, Song B, Xiong Y, Lu J (2014) Immunosuppressive activity of alpinetin on activation and cytokines secretion of murine t lymphocytes. Immunopharmacol Immunotoxicol 36:290–296CrossRefPubMed

Hooshmand S, Kumar A, Zhang JY, Johnson SA, Chai SC, Arjmandi BH (2015) Evidence for anti-inflammatory and antioxidative properties of dried plum polyphenols in macrophage RAW 264.7 cells. Food Funct 6:1719–1725CrossRefPubMed

Hu W, Wang X, Wu L, Shen T, Ji L, Zhao X, Si CL, Jiang Y, Wang G (2016) Apigenin-7-O-β-d-glucuronide inhibits LPS-induced inflammation through the inactivation of AP-1 and MAPK signaling pathways in RAW 264.7 macrophages and protects mice against endotoxin shock. Food Funct 7:1002–1013CrossRefPubMed

Itoh T, Ando M, Tsukamasa Y, Wakimoto T, Nukaya H (2012) Whiskey congeners suppress LPS/IFNγ-induced NO production in murine macrophage RAW 264 cells by inducing heme oxygenase-1 expression. J Agric Food Chem 60:12491–12500CrossRefPubMed

Jaiswal R, Karaköse H, Rühmann S, Goldner K, Neumüller M, Treutter D, Kuhnert N (2013) Identification of phenolic compounds in plum fruits (Prunus salicina L. and Prunus domestica L.) by high-performance liquid chromatography/tandem mass spectrometry and characterization of varieties by quantitative phenolic fingerprints. J Agric Food Chem 61:12020–12031CrossRefPubMed

Jakobek L, Šeruga M, Šeruga B, Novak I, Medvidović-Kosanović M (2009) Phenolic compound composition and antioxidant activity of fruits of Rubus and Prunus species from Croatia. Int J Food Sci 44:860–868CrossRef

Jena AK, Vasisht K, Sharma N, Kaur R, Dhingra MS, Karan M (2016) Amelioration of testosterone induced benign prostatic hyperplasia by Prunus species. J Ethnopharmacol 190:33–45CrossRefPubMed

Joseph N, Anjum N, Tripathi YC (2016) Phytochemical screening and evaluation of polyphenols, flavonoids and antioxidant activity of Prunus cerasoides D. Don Leaves. J Pharm Res 10:502–508

Joshi R, Rana A, Gulati A (2015) Studies on quality of orthodox teas made from anthocyanin-rich tea clones growing in Kangra valley, India. Food Chem 176:357–366CrossRefPubMed

Kanwar JR, Kanwar RK, Burrow H, Baratchi S (2009) Recent advances on the roles of NO in cancer and chronic inflammatory disorders. Curr Med Chem 16:2373–2394CrossRefPubMed

Kuo YC, Meng HC, Tsai WJ (2001) Regulation of cell proliferation, inflammatory cytokine production and calcium mobilization in primary human T lymphocytes by emodin from Polygonum hypoleucum Ohwi. Inflamm Res 50:73–82CrossRefPubMed

Laavola M, Haavikko R, Hämäläinen M, Leppänen T, Nieminen R, Alakurtti S, Moreira VM, Yli-Kauhaluoma J, Moilanen E (2016) Betulin derivatives effectively suppress inflammation in vitro and in vivo. J Nat Prod 79:274–280CrossRefPubMed

Lazarini JG, Franchin M, Infante J, Paschoal JAR, Freires IA, de Alencar SM, Rosalen PL (2016) Anti-inflammatory activity and polyphenolic profile of the hydroalcoholic seed extract of Eugenia leitonii, an unexplored Brazilian native fruit. J Funct Foods 26:249–257CrossRef

Lee IA, Hyam SR, Jang SE, Han MJ, Kim DH (2012) Ginsenoside Re ameliorates inflammation by inhibiting the binding of lipopolysaccharide to TLR4 on macrophages. J Agric Food Chem 60:9595–9602CrossRefPubMed

Levaj B, Dragović-Uzelac V, Delonga K, Kovačević Ganić K, Banović M, Bursać Kovačević D (2010) Polyphenols and volatiles in fruits of two sour cherry cultivars, some berry fruits and their jams. Food Technol Biotechnol 248:538–547

Lima GPP, Vianello F, Corrêa CR, Campos RADS, Borguini MG (2014) Polyphenols in fruits and vegetables and its effect on human health. Food Sci Nutr 5:1065–1082

Liu Y, Su WW, Wang S, Li PB (2012) Naringin inhibits chemokine production in an LPS-induced RAW 264.7 macrophage cell line. Mol Med Rep 6:1343–1350CrossRefPubMed

Malsawmtluangi C, Thanzami K, Lalhlenmawia H, Selvan V, Palanisamy S, Kandasamy R, Pachuau L (2014) Physicochemical characteristics and Antioxidant activity of Prunus cerasoides D. Don gum exudates. Int J Biol Macromol 69:192–199CrossRefPubMed

Maroon JC, Bost JW, Maroon A (2010) Natural anti-inflammatory agents for pain relief. Surg Neurol Int 1:80CrossRefPubMedPubMedCentral

Noratto GD, Angel-Morales G, Talcott ST, Mertens-Talcott SU (2011) Polyphenolics from acai (Euterpe oleracea Mart.) and red muscadine grape (Vitis rotundifolia) protect human umbilical vascular endothelial cells (HUVEC) from glucose-and lipopolysaccharide (LPS)-induced inflammation and target microRNA-126. J Agric Food Chem 59:7999–8012CrossRefPubMed

Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:337–341CrossRefPubMed

Rana S, Kumar S, Rana A, Sharma V, Katoch P, Padwad Y, Bhushan S (2016) Phenolic constituents from apple tree leaves and their in vitro biological activity. Ind Crop Prod 90:118–125CrossRef

Sareila O, Korhonen R, Auvinen H, Hämäläinen M, Kankaanranta H, Nissinen E, Moilanen E (2008) Effects of levo-and dextrosimendan on NF-κB-mediated transcription, iNOS expression and NO production in response to inflammatory stimuli. Br J Pharmacol 155:884–895CrossRefPubMedPubMedCentral

Shahid A, Ali R, Ali N, Hasan SK, Bernwal P, Afzal SM, Vafa A, Sultana S (2016) Modulatory effects of catechin hydrate against genotoxicity, oxidative stress, inflammation and apoptosis induced by benzo (a) pyrene in mice. Food Chem Toxicol 92:64–74CrossRefPubMed

Sharma R, Tiku AB (2016) Emodin inhibits splenocyte proliferation and inflammation by modulating cytokine responses in a mouse model system. J Immunotoxicol 13:20–26CrossRefPubMed

Sharma JN, Al-Omran A, Parvathy SS (2007) Role of nitric oxide in inflammatory diseases. Inflammopharmacology 15:252–259CrossRefPubMed

Sharma R, Kapila R, Haq MRU, Salingati V, Kapasiya M, Kapila S (2014) Age-associated aberrations in mouse cellular and humoral immune responses. Aging Clin Exp Res 26:353–362CrossRefPubMed

Sharma R, Sharma A, Kumari A, Kulurkar PM, Raj R, Gulati A, Padwad YS (2017) Consumption of green tea epigallocatechin-3-gallate enhances systemic immune response, antioxidative capacity and HPA axis functions in aged male swiss albino mice. Biogerontology 2017:367–382CrossRef

Singh H, Lily MK, Dangwal K (2015) Evaluation and comparison of polyphenols and bioactivities of wild edible fruits of north-west himalaya, India. Asian Pac J Trop Dis 5:888–893CrossRef

Tolando R, Jovanović A, Brigelius-Flohé R, Ursini F, Maiorino M (2000) Reactive oxygen species and proinflammatory cytokine signaling in endothelial cells: effect of selenium supplementation. Free Radic Biol Med 28:979–986CrossRefPubMed

Vasco C, Ruales J, Kamal-Eldin A (2008) Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chem 111:816–823CrossRef

Wang Y, Li B, Zhu J, Zhang Q, Zhang X, Li L, Ma Y, Meng X (2016) Lonicera caerulea berry extract suppresses lipopolysaccharide-induced inflammation via toll-like receptor and oxidative stress-associated mitogen-activated protein kinase signaling. Food Funct 7:4267–4277CrossRefPubMed

Xiong Y, Chen D, Yu C, Lv B, Peng J, Wang J, Lin Y (2015) Citrus nobiletin ameliorates experimental colitis by reducing inflammation and restoring impaired intestinal barrier function. Mol Nutr Food Res 59:829–842CrossRefPubMed

Yan H, Wang H, Zhang X, Li X, Yu J (2015) Ascorbic acid ameliorates oxidative stress and inflammation in dextran sulfate sodium-induced ulcerative colitis in mice. Int J Clin Exp Med 8:20245PubMedCentralPubMed

Zhou T, Xu DP, Lin SJ, Li Y, Zheng J, Zhou Y, Zhang JJ, Li HB (2017) Ultrasound-assisted extraction and identification of natural antioxidants from the fruit of Melastoma sanguineum sims. Molecules 22:306–321CrossRefPubMedCentral

Titel: Prunus cerasoides fruit extract ameliorates inflammatory stress by modulation of iNOS pathway and Th1/Th2 immune homeostasis in activated murine macrophages and lymphocytes
verfasst von: Anamika Sharma
Robin Joshi
Shiv Kumar
Rohit Sharma
Rajneesh
Yogendra Padwad
Mahesh Gupta
Publikationsdatum: 10.02.2018
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 6/2018
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-018-0448-2

Springer Medizin

Abstract

Graphical Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2018

Ex vivo immunomodulatory effect of ethanolic extract of propolis during Celiac Disease: involvement of nitric oxide pathway

Anti-inflammatory and anti-arthritic effects of methanol extract of the stem bark of Boswellia dalzielii Hutch (Burseraceae) in rats

The significant impact of apigenin on different aspects of autoimmune disease

Nyctanthes arbor-tristis leaf extract ameliorates hyperlipidemia- and hyperglycemia-associated nephrotoxicity by improving anti-oxidant and anti-inflammatory status in high-fat diet–streptozotocin-induced diabetic rats

Quinazoline clubbed 1,3,5-triazine derivatives as VEGFR2 kinase inhibitors: design, synthesis, docking, in vitro cytotoxicity and in ovo antiangiogenic activity

Involvement of central opioid receptors in protective effects of methadone on experimental colitis in rats