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Journal of Cancer Research and Clinical Oncology

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01.04.2007 | Original Paper

Psoriasin (S100A7) is significantly up-regulated in human epithelial skin tumours

verfasst von: Noemi Moubayed, Michael Weichenthal, Jürgen Harder, Elke Wandel, Michael Sticherling, Regine Gläser

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 4/2007

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Abstract

Purpose

Psoriasin (S100A7) has originally been described to be expressed by psoriatic keratinocytes possibly as a result of altered differentiation and inflammation. As psoriasin was found to be overexpressed in human breast and bladder cancer suggesting a role in tumour progression, we investigated the expression of psoriasin in human epithelial skin tumours.

Methods

Realtime reverse transcription-polymerase chain reaction experiments were performed to analyse the mRNA-expression levels of psoriasin together with involucrin as a marker for epithelial differentiation and interleukin-8 (IL-8) as a marker for inflammation in skin biopsy samples from patients with precancerous skin lesions (PSL, n = 6), squamous cell carcinoma (SCC, n = 11), basal cell carcinoma (BCC, n = 17), and healthy controls (n = 10).

Results

Unexpectedly, mRNA expression levels for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) revealed a variation of up to 600-fold in all cDNA-samples under investigation, indicating that GAPDH is not suitable as a housekeeping gene in human skin samples. Psoriasin mRNA expression was significantly up-regulated in samples of PSL, SCC and BCC. In situ hybridisation and immunohistochemical examinations identified psoriasin mRNA and protein expression in the differentiated layers of the epidermis. IL-8 mRNA expression was significantly up-regulated in SCC, however, there was no correlation between elevated levels of psoriasin and the expression of IL-8.

Conclusion

Similar to the findings in breast and bladder cancer, the up-regulation of psoriasin might play a role in the progression of skin cancer. The expression of psoriasin in human skin tumours seems to be independent from differentiation and inflammation.

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Titel: Psoriasin (S100A7) is significantly up-regulated in human epithelial skin tumours
verfasst von: Noemi Moubayed
Michael Weichenthal
Jürgen Harder
Elke Wandel
Michael Sticherling
Regine Gläser
Publikationsdatum: 01.04.2007
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 4/2007
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-006-0164-y

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16.04.2024 | Maligne primäre ZNS-Tumoren | Nachrichten

Springer Medizin

Psoriasin (S100A7) is significantly up-regulated in human epithelial skin tumours

Abstract

Purpose

Methods

Results

Conclusion

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Conclusion

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