Background
Results
Discontinuous density gradient purification of oocysts
Separating oocysts from contaminants using flow cytometry
Genomic DNA extraction from purified oocysts
Bioinformatic analysis of Illumina reads
Purification status of oocysts | Number of mapped reads/total number of reads = % of reads mapped to HCNY assembly | ||
---|---|---|---|
CDC:HCRI01:97 | CDC:HCGM01:97 | CDC:HCTX69:14 | |
Before flow sorting | 2,262,396/9,739,022 = 23% | 1,781,583/9,315,212 = 19% | 163,179/2,767,726 = 6% |
Flow cytometry sorted | 6,282,480/7,785,610 = 81% | 5,954,094/6,137,902 = 97% | 2,486,477/2,888,1657 = 86% |
De novo assembly of draft genomes of C. cayetanensis samples
Total sequence length (bp) | Number of contigs (> 500 bp) | Contig N50 | Contig L50 | G+C content (%) | |
---|---|---|---|---|---|
CHN_HEN01 [14] | 44,034,411 | 3573 | 43,794 | 282 | 52 |
HCNY16:01 [13] | 44,563,857 | 865 | 187,023 | 72 | 52 |
HCDC004_96 | 44,485,136 | 1120 | 142,454 | 92 | 52 |
HCGM002_97 | 44,562,363 | 1073 | 145,614 | 91 | 52 |
HCGM011_97 | 44,518,937 | 677 | 235,157 | 64 | 52 |
HCGM012_97 | 44,435,629 | 2796 | 37,553 | 352 | 52 |
HCNP016_97 | 44,777,959 | 2659 | 62,977 | 203 | 52 |
HCRI001_97 | 45,472,771 | 1526 | 136,687 | 101 | 51 |
HCTX119_13 | 43,837,200 | 1503 | 83,084 | 160 | 52 |
HCTX365_13 | 44,340,945 | 1646 | 86,734 | 155 | 52 |
HCJK001_14 | 44,286,668 | 669 | 180,727 | 72 | 52 |
HCTX535_14 | 44,523,203 | 1266 | 130,032 | 104 | 52 |
HCTX569_14 | 44,347,388 | 792 | 202,065 | 71 | 52 |
HCJK008_15 | 44,551,431 | 1570 | 104,384 | 140 | 52 |
HCJK011_15 | 44,458,373 | 1289 | 112,390 | 114 | 52 |
HCJK015_15 | 44,350,146 | 2827 | 36,648 | 354 | 52 |
HCTX542_15 | 44,626,029 | 1426 | 147,450 | 94 | 52 |
HCTX547_15 | 44,368,440 | 1711 | 81,750 | 169 | 52 |
HCMX010_16 | 44,405,937 | 816 | 218,769 | 59 | 52 |
HCNE181_16 | 44,372,150 | 1421 | 100,417 | 134 | 52 |
HCTX460_16 | 44,233,679 | 1640 | 82,105 | 162 | 52 |
HCTX495_16 | 44,243,943 | 2776 | 38,624 | 336 | 52 |
HCTX503_16 | 44,362,393 | 1790 | 79,041 | 181 | 52 |