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Journal of Cancer Research and Clinical Oncology

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01.01.2012 | Original Paper

Quantitative methylation analysis of HOXA3, 7, 9, and 10 genes in glioma: association with tumor WHO grade and clinical outcome

verfasst von: Angela Di Vinci, Ida Casciano, Elena Marasco, Barbara Banelli, Gian Luigi Ravetti, Luana Borzì, Claudio Brigati, Alessandra Forlani, Alessandra Dorcaratto, Giorgio Allemanni, Gianluigi Zona, Renato Spaziante, Henning Gohlke, Giovanni Gardin, Domenico Franco Merlo, Vilma Mantovani, Massimo Romani

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 1/2012

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Abstract

Purpose

The purpose of this study was to determine whether specific HOXA epigenetic signatures could differentiate glioma with distinct biological, pathological, and clinical characteristics.

Methods

We evaluated HOXA3, 7, 9, and 10 methylation in 63 glioma samples by MassARRAY and pyrosequencing.

Results

We demonstrated the direct statistical correlation between the level of methylation of all HOXA genes examined and WHO grading. Moreover, in glioblastoma patients, higher level of HOXA9 and HOXA10 methylation significantly correlated with increased survival probability (HOXA9—HR: 0.36, P = 0.007; HOXA10—HR: 0.46, P = 0.045; combined HOXA9 and 10—HR 0.28, P = 0.004).

Conclusions

This study identifies HOXA3, 7, 9, and 10 as methylation targets mainly in high-grade glioma and hypermethylation of the HOXA9 and 10 as prognostic factor in glioblastoma patients. Our data indicate that these epigenetic changes may be biomarkers of clinically different subgroups of glioma patients that could eventually benefit from personalized therapeutic strategies.

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Titel: Quantitative methylation analysis of HOXA3, 7, 9, and 10 genes in glioma: association with tumor WHO grade and clinical outcome
verfasst von: Angela Di Vinci
Ida Casciano
Elena Marasco
Barbara Banelli
Gian Luigi Ravetti
Luana Borzì
Claudio Brigati
Alessandra Forlani
Alessandra Dorcaratto
Giorgio Allemanni
Gianluigi Zona
Renato Spaziante
Henning Gohlke
Giovanni Gardin
Domenico Franco Merlo
Vilma Mantovani
Massimo Romani
Publikationsdatum: 01.01.2012
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 1/2012
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-011-1070-5

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Quantitative methylation analysis of HOXA3, 7, 9, and 10 genes in glioma: association with tumor WHO grade and clinical outcome

Abstract

Purpose

Methods

Results

Conclusions

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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