Background
Materials and methods
Ethics statement
Study population and sample statement
Protein preparation and iTRAQ labeling
Liquid chromatography mass spectrometry (LC-MS) analysis
Protein identification and quantitation
Protein-protein interaction analysis conducted on GeneMANIA
Protein function and pathway enrichment analysis
Protein selection and validation using western blot
Results
Protein expression profiling and identification of differentially expressed proteins
Uniprot ID | Protein Name | Gene Name | Log2FC | P value |
---|---|---|---|---|
Up-regulated proteins | ||||
P04279 | Semenogelin-1 | SEMG1 | 2.27 | 1.51E-08 |
P02751 | Fibronectin | FN1 | 1.24 | 7.50268E-05 |
A0A0U1RQF0 | Fatty acid synthase | FASN | 1.21 | 2.09185E-06 |
E7EQB2 | Lactotransferrin | LTF | 2.14 | 3.04345E-11 |
P35579 | Myosin-9 | MYH9 | 1.36 | 1.05659E-05 |
Q02383 | Semenogelin-2 | SEMG2 | 1.62 | 0.027249441 |
O75369 | Filamin-B | FLNB | 0.73 | 0.033877425 |
P50991 | T-complex protein 1 | CCT4 | 1.06 | 0.017997051 |
P53396 | ATP-citrate synthase | ACLY | 0.84 | 0.038844213 |
P35580 | Myosin-10 | MYH10 | 1.12 | 0.002368773 |
P12277 | Creatine kinase B | CKB | 1.29 | 0.002233567 |
P62258 | 14–3-3 protein epsilon | YWHAE | 1.09 | 0.033955991 |
P27487 | Dipeptidyl peptidase 4 | DPP4 | 0.85 | 0.016909158 |
Q00796 | Sorbitol dehydrogenase | SORD | 1.77 | 0.01105386 |
P12273 | Prolactin-inducible protein | PIP | 1.75 | 0.014142492 |
Q6UX06 | Olfactomedin-4 | OLFM4 | 2.03 | 0.041974943 |
P49221 | Glutamyltransferase 4 | TGM4 | 1.85 | 0.048483204 |
P08670 | Vimentin | VIM | 0.98 | 0.023722751 |
O43175 | Phosphoglycerate dehydrogenase | PHGDH | 2.01 | 0.01025431 |
P31947 | 14–3-3 protein sigma | SFN | 1.01 | 0.011000973 |
P09525 | Annexin A4 | ANXA4 | 1.25 | 0.021621821 |
O43776 | Asparagine--tRNA ligase | NARS | 1.29 | 0.031847354 |
P47895 | Aldehyde dehydrogenase | ALDH1A3 | 1.42 | 0.045620359 |
X6RBG4 | Uromodulin | UMOD | 2.74 | 0.043337822 |
C9JC07 | Myeloid-associated differentiation marker | MYADM | 5.50 | 0.013526945 |
Down-regulated proteins | ||||
Q5JQC9 | A-kinase anchor protein 4 | AKAP4 | −1.50 | 1.12E-09 |
O75969 | A-kinase anchor protein 3 | AKAP3 | −1.25 | 1.17548E-05 |
P19367 | Hexokinase-1 | HK1 | −0.60 | 0.002458935 |
Q8NEB7 | Acrosin-binding protein | ACRBP | −0.82 | 0.03690489 |
J3QTJ6 | Fibrous sheath-interacting protein 2 | FSIP2 | −1.18 | 2.85668E-06 |
O75976 | Carboxypeptidase D | CPD | −0.78 | 0.004574668 |
Q8TEX9 | Importin-4 | IPO4 | −1.20 | 0.002037515 |
Q8TC56 | Protein FAM71B | FAM71B | −0.92 | 0.00900713 |
Q9BXF9 | Tektin-3 | TEKT3 | −0.70 | 0.016060278 |
A0A0C4DGB6 | Serum albumin | ALB | −1.28 | 0.006274223 |
A0A0G2JML4 | C6orf10 | C6orf10 | −1.01 | 0.041954588 |
Q6ZU69 | Protein FAM205A | FAM205A | −1.26 | 0.010902458 |
Q9UII2 | ATPase inhibitor, mitochondrial | ATPIF1 | −1.01 | 0.035165988 |