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15.05.2017 | Original Article

Quercetin inhibits gout arthritis in mice: induction of an opioid-dependent regulation of inflammasome

verfasst von: Kenji W. Ruiz-Miyazawa, Larissa Staurengo-Ferrari, Sandra S. Mizokami, Talita P. Domiciano, Fabiana T. M. C. Vicentini, Doumit Camilios-Neto, Wander R. Pavanelli, Phileno Pinge-Filho, Flávio A. Amaral, Mauro M. Teixeira, Rubia Casagrande, Waldiceu A. Verri Jr.

Erschienen in: Inflammopharmacology | Ausgabe 5/2017

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Abstract

We investigated the anti-inflammatory and analgesic effects of quercetin in monosodium urate crystals (MSU)-induced gout arthritis, and the sensitivity of quercetin effects to naloxone, an opioid receptor antagonist. Mice were treated with quercetin, and mechanical hyperalgesia was assessed at 1–24 h after MSU injection. In vivo, leukocyte recruitment, cytokine levels, oxidative stress, NFκB activation, and gp91^phox and inflammasome components (NLRP3, ASC, Pro-caspase-1, and Pro-IL-1β) mRNA expression by qPCR were determined in the knee joints at 24 h after MSU injection. Inflammasome activation was determined, in vitro, in lipopolysaccharide-primed macrophages challenged with MSU. Quercetin inhibited MSU-induced mechanical hyperalgesia, leukocyte recruitment, TNFα and IL-1β production, superoxide anion production, inflammasome activation, decrease of antioxidants levels, NFκB activation, and inflammasome components mRNA expression. Naloxone pre-treatment prevented all the inhibitory effects of quercetin over MSU-induced gout arthritis. These results demonstrate that quercetin exerts analgesic and anti-inflammatory effect in the MSU-induced arthritis in a naloxone-sensitive manner.

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Titel: Quercetin inhibits gout arthritis in mice: induction of an opioid-dependent regulation of inflammasome
verfasst von: Kenji W. Ruiz-Miyazawa
Larissa Staurengo-Ferrari
Sandra S. Mizokami
Talita P. Domiciano
Fabiana T. M. C. Vicentini
Doumit Camilios-Neto
Wander R. Pavanelli
Phileno Pinge-Filho
Flávio A. Amaral
Mauro M. Teixeira
Rubia Casagrande
Waldiceu A. Verri Jr.
Publikationsdatum: 15.05.2017
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 5/2017
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0356-x

Springer Medizin

Abstract

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