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2016 | Hauterkrankungen in der Hausarztpraxis | OriginalPaper | Buchkapitel

8. Raman-Spektroskopie in der Dermatologie

verfasst von : Jürgen Lademann, Prof. Dr. med., Martina C. Meinke, PD Dr. rer. nat., Alexa Patzelt, Dr. med., Maxim E. Darvin, Dr. rer. med.

Erschienen in: Nichtinvasive physikalische Diagnostik in der Dermatologie

Verlag: Springer Berlin Heidelberg

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Zusammenfassung

Die Dermatologie ist ein geeignetes Fachgebiet, um optische und spektroskopische Untersuchungen zur Analyse der Haut, aber auch zur Penetration topisch applizierter Substanzen durchzuführen. Der Penetration topisch applizierter Substanzen in Form von Arzneimitteln und Pflegeprodukten kommt hierbei eine besondere Bedeutung zu. Während laserscanmikroskopische Untersuchungen hauptsächlich dazu dienen, die zelluläre Struktur abzubilden, wird mit Hilfe der Raman-Spektroskopie die inelastische Streuung von Licht an Molekülen oder Festkörpern erfasst. Damit ermöglicht die Raman-Spektroskopie den Nachweis von Stoffen in und auf der Haut. Hierbei wird Laserlicht in die Haut eingestrahlt. Neben der eingestrahlten Frequenz werden noch weitere Frequenzen beobachtet, deren Unterschiede zur Frequenz des eingestrahlten Lichts Informationen über die Rotationsschwingungs- und Photonen- oder Spin-Flip-Prozesse liefern. Mit den erhaltenen Spektren können Substanzen selektiv nachgewiesen werden.

Bernstein PS, Zhao DY, Sharifzadeh M, Ermakov IV, Gellermann W (2004) Resonance Raman measurement of macular carotenoids in the living human eye. Arch Biochem Biophys 430(2):163–169CrossRefPubMed

Bhattacharjee T, Kumar P, Maru G, Ingle A, Krishna CM (2014) Swiss bare mice: a suitable model for transcutaneous in vivo Raman spectroscopic studies of breast cancer. Laser Med Sci 29(1):325–333CrossRef

Blum A, Rassner G, Garbe C (2003) Modified ABC-point list of dermoscopy: A simplified and highly accurate dermoscopic algorithm for the diagnosis of cutaneous melanocytic lesions. J Am Acad Dermatol 48(5):672–678CrossRefPubMed

Blume-Peytavi U, Rolland A, Darvin ME, Constable A, Pineau I, Voit C, Zappel K, Schafer-Hesterberg G, Meinke M, Clavez RL, Sterry W, Lademann J (2009) Cutaneous lycopene and beta-carotene levels measured by resonance Raman spectroscopy: high reliability and sensitivity to oral lactolycopene deprivation and supplementation. Eur J Pharm Biopharm 73(1):187–194CrossRefPubMed

Bodanese B, Silveira FL, Zangaro RA, Pacheco MTT, Pasqualucci CA, Silveira L (2012) Discrimination of basal cell carcinoma and melanoma from normal skin biopsies in vitro through Raman spectroscopy and principal component analysis. Photomed Laser Surg 30(7):381–387PubMedCentralCrossRefPubMed

Breunig HG, Buckle R, Kellner-Hofer M, Weinigel M, Lademann J, Sterry W, Konig K (2012) Combined in vivo multiphoton and CARS imaging of healthy and disease-affected human skin. Microsc Res Techniq 75(4):492–498CrossRef

Cartaxo SB, Santos IDDO, Bitar R, Oliveira AF, Ferreira LM, Martinho HS, Martin AA (2010) FT-Raman spectroscopy for the differentiation between cutaneous melanoma and pigmented nevus. Acta Cir Bras 25(4):351–356CrossRefPubMed

Darvin M, Patzelt A, Gehse S, Schanzer S, Benderoth C, Sterry W, Lademann J (2008a) Cutaneous concentration of lycopene correlates significantly with the roughness of the skin. Eur J Pharm Biopharm 69(3):943–947CrossRefPubMed

Darvin ME, Fluhr JW, Caspers P, van der Pool A, Richter H, Patzelt A, Sterry W, Lademann J (2009) In vivo distribution of carotenoids in different anatomical locations of human skin: comparative assessment with two different Raman spectroscopy methods. Exp Dermatol 18(12):1060–1063CrossRefPubMed

Darvin ME, Fluhr JW, Meinke MC, Zastrow L, Sterry W, Lademann J (2011) Topical beta-carotene protects against infra-red-light-induced free radicals. Exp Dermatol 20(2):125–129CrossRefPubMed

Darvin ME, Gersonde I, Albrecht H, Sterry W, Lademann J (2006) In vivo Raman spectroscopic analysis of the influence of UV radiation on carotenoid antioxidant substance degradation of the human skin. Laser Phys 16(5):833–837CrossRef

Darvin ME, Konig K, Kellner-Hoefer M, Breunig HG, Werncke W, Meinke MC, Patzelt A, Sterry W, Lademann J (2012a) Safety assessment by multiphoton fluorescence/second harmonic generation/hyper-rayleigh scattering tomography of ZnO nanoparticles used in cosmetic products. Skin Pharmacol Physiol 25(4):219–226CrossRefPubMed

Darvin ME, Meinke MC, Sterry W, Lademann J (2013a) Optical methods for noninvasive determination of carotenoids in human and animal skin. J Biomed Opt 18(6):61230CrossRefPubMed

Darvin ME, Patzelt A, Knorr F, Blume-Peytavi U, Sterry W, Lademann J (2008b) One-year study on the variation of carotenoid antioxidant substances in living human skin: influence of dietary supplementation and stress factors. J Biomed Opt 13(4):044028CrossRefPubMed

Darvin ME, Sandhagen C, Koecher W, Sterry W, Lademann J, Meinke MC (2012b) Comparison of two methods for noninvasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy. J Biophotonics 5(7):550–558CrossRefPubMed

Darvin ME, Sterry W, Lademann J, Patzelt A (2013b) Alcohol consumption decreases the protection efficiency of the antioxidant network and increases the risk of sunburn in human skin. Skin Pharmacol Physiol 26(1):45–51CrossRefPubMed

Drutis DM, Hancewicz TM, Pashkovski E, Feng L, Mihalov D, Holtom G, Ananthapadmanabhan KP, Xie XS, Misra M (2014) Three-dimensional chemical imaging of skin using stimulated Raman scattering microscopy. J Biomed Opt 19(11):111604CrossRefPubMed

Emanuele E, Spencer JM, Braun M (2014) From DNA repair to proteome protection: new molecular insights for preventing non-melanoma skin cancers and skin aging. J Drugs Dermatol 13:274–281PubMed

Ermakov IV, Gellermann W (2015) Optical detection methods for carotenoids in human skin. Arch Biochem Biophys 572:101–111CrossRefPubMed

Fernandez-Garcia E (2014) Skin protection against UV light by dietary antioxidants. Food Funct 5(9):1994–2003CrossRefPubMed

Franzen L, Anderski J, Planz V, Kostka KH, Windbergs M (2014) Combining confocal Raman microscopy and freeze-drying for quantification of substance penetration into human skin. Experimental Dermatology 23(12):942–944CrossRefPubMed

Franzen L, Mathes C, Hansen S, Windbergs M (2013a) Advanced chemical imaging and comparison of human and porcine hair follicles for drug delivery by confocal Raman microscopy. J Biomed Opt 18(6):061210CrossRefPubMed

Franzen L, Selzer D, Fluhr JW, Schaefer UF, Windbergs M (2013b) Towards drug quantification in human skin with confocal Raman microscopy. Eur J Pharm Biopharm 84(2):437–444CrossRefPubMed

Freedberg IM (2003) Fitzpatrick’s dermatology in general medicine, 6. Aufl. McGraw-Hill, New York, S XXXIII (1357)

Fu D, Lu FK, Zhang X, Freudiger C, Pernik DR, Holtom G, Xie XS (2012) Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy. J Am Chem Soc 134(8):3623–3626PubMedCentralCrossRefPubMed

Gellermann W, Bernstein PS (2004) Noninvasive detection of macular pigments in the human eye. J Biomed Opt 9(1):75–85CrossRefPubMed

Gniadecka M, Philipsen PA, Sigurdsson S, Wessel S, Nielsen OF, Christensen DH, Hercogova J, Rossen K, Thomsen HK, Gniadecki R, Hansen LK, Wulf HC (2004) Melanoma diagnosis by Raman spectroscopy and neural networks: structure alterations in proteins and lipids in intact cancer tissue. J Invest Dermatol 122(2):443–449CrossRefPubMed

Haag SF, Bechtel A, Darvin ME, Klein F, Groth N, Schafer-Korting M, Bittl R, Lademann J, Sterry W, Meinke MC (2010) Comparative study of carotenoids, catalase and radical formation in human and animal skin. Skin Pharmacol Physiol 23(6):306–312CrossRefPubMed

Hesterberg K, Lademann J, Patzelt A, Sterry W, Darvin ME (2009) Raman spectroscopic analysis of the increase of the carotenoid antioxidant concentration in human skin after a 1-week diet with ecological eggs. J Biomed Opt 14(2):024039CrossRefPubMed

Hoppel M, Holper E, Baurecht D, Valenta C (2015) Monitoring the distribution of surfactants in the stratum corneum by combined ATR-FTIR and tape-stripping experiments. Skin Pharmacol Physiol 28(3):167–175CrossRefPubMed

Ismail N, Choo-Smith LP, Worhoff K, Driessen A, Baclig AC, Caspers PJ, Puppels GJ, de Ridder RM, Pollnau M (2011) Raman spectroscopy with an integrated arrayed-waveguide grating. Opt Lett 36(23):4629–4631CrossRefPubMed

Kardynal A, Olszewska M (2014) Modern non-invasive diagnostic techniques in the detection of early cutaneous melanoma. J Dermatol Case Rep 8(1):1–8PubMedCentralCrossRefPubMed

Kittler H, Pehamberger H, Wolff K, Binder M (2002) Diagnostic accuracy of dermoscopy. The Lancet Oncology 3(3):159–165CrossRefPubMed

Klein J, Darvin ME, Meinke MC, Schweigert FJ, Muller KE, Lademann J (2013) Analyses of the correlation between dermal and blood carotenoids in female cattle by optical methods. J Biomed Opt 18(6):061219CrossRef

Klein J, Darvin ME, Muller KE, Lademann J (2012) Serial non-invasive measurements of dermal carotenoid concentrations in dairy cows following recovery from abomasal displacement. Plos One 7(10):e47706PubMedCentralCrossRefPubMed

Lademann J, Jacobi U, Surber C, Weigmann HJ, Fluhr JW (2009) The tape stripping procedure – evaluation of some critical parameters. Eur J Pharm Biopharm 72(2):317–323CrossRefPubMed

Lademann J, Meinke MC, Schanzer S, Richter H, Darvin ME, Haag SF, Fluhr JW, Weigmann HJ, Sterry W, Patzelt A (2012) In vivo methods for the analysis of the penetration of topically applied substances in and through the skin barrier. Int J Cosmetic Sci 34(6):551–559CrossRef

Lademann J, Patzelt A, Schanzer S, Richter H, Meinke MC, Sterry W, Zastrow L, Doucet O, Vergou T, Darvin ME (2011a) Uptake of antioxidants by natural nutrition and supplementation: pros and cons from the dermatological point of view. Skin Pharmacol Physiol 24(5):269–273CrossRefPubMed

Lademann J, Schanzer S, Meinke M, Sterry W, Darvin ME (2011b) Interaction between carotenoids and free radicals in human skin. Skin Pharmacol Physiol 24(5):238–244CrossRefPubMed

Li SC, Tachiki LML, Kabeer MH, Dethlefs BA, Anthony MJ, Loudon WG (2014) Cancer genomic research at the crossroads: realizing the changing genetic landscape as intratumoral spatial and temporal heterogeneity becomes a confounding factor. Cancer Cell Int 14:115PubMedCentralCrossRefPubMed

Lieber C, Mahadevan-Jansen A (2007) Development of a handheld Raman microspectrometer for clinical dermatologic applications. Optics express 15(19):11874–11882CrossRefPubMed

Lieber CA, Majumder SK, Billheimer D, Ellis DL, Mahadevan-Jansen A (2008a) Raman microspectroscopy for skin cancer detection in vitro. J Biomed Opt 13(2):024013CrossRefPubMed

Lieber CA, Majumder SK, Ellis DL, Billheimer DD, Mahadevan-Jansen A (2008b) In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy. Laser Surg Med 40(7):461–467CrossRef

Lindemann U, Wilken K, Weigmann HJ, Schaefer H, Sterry W, Lademann J (2003) Quantification of the horny layer using tape stripping and microscopic techniques. J Biomed Opt 8(4):601–607CrossRefPubMed

Mahadevan-Jansen A, Mitchell MF, Ramanujam N, Utzinger U, Richards-Kortum R (1998) Development of a fiber optic probe to measure NIR Raman spectra of cervical tissue in vivo. Photochem Photobiol 68(3):427–431CrossRefPubMed

Mittal R, Balu M, Krasieva T, Potma EO, Elkeeb L, Zachary CB, Wilder-Smith P (2013) Evaluation of stimulated Raman scattering microscopy for identifying squamous cell carcinoma in human skin. Laser Surg Med 45(8):496–502

Mogensen M, Jemec GBE (2007) Diagnosis of nonmelanoma skin cancer/keratinocyte carcinoma: a review of diagnostic accuracy of nonmelanoma skin cancer diagnostic tests and technologies. Dermatol Surg 33(10):1158–1174PubMed

Motz JT, Hunter M, Galindo LH, Gardecki JA, Kramer JR, Dasari RR, Feld MS (2004) Optical fiber probe for biomedical Raman spectroscopy. Appl Opt 43(3):542–554CrossRefPubMed

Nijssen A, Koljenovic S, Bakker STC, Caspers PJ, Puppels GJ (2009) Towards oncological application of Raman spectroscopy. J Biophotonics 2(1–2):29–36CrossRefPubMed

Philipsen PA, Knudsen L, Gniadecka M, Ravnbak MH, Wulf HC (2013) Diagnosis of malignant melanoma and basal cell carcinoma by in vivo NIR-FT Raman spectroscopy is independent of skin pigmentation. Photoch Photobio Sci 12(5):770–776CrossRef

Rohr M, Klette E, Ruppert S, Bimzcok R, Klebon B, Heinrich U, Tronnier H, Johncock W, Peters S, Pflucker F, Rudolph T, Flosser-Muller H, Jenni K, Kockott D, Lademann J, Herzog B, Bielfeldt S, Mendrok-Edinger C, Hanay C, Zastrow L (2010) In vitro sun protection factor: still a challenge with no final answer. Skin Pharmacol Physiol 23(4):201–212CrossRefPubMed

Schleusener J, Gluszczynska P, Reble C, Gersonde I, Helfmann J, Cappius HJ, Fluhr JW, Meinke MC (2015a) Discrimination of basal cell carcinoma and melanoma from normal skin biopsies in vitro through raman spectroscopy and principal component analysis. Appl Spectrosc 69(2):13CrossRef

Schleusener J, Gluszczynska P, Reble C, Gersonde I, Helfmann J, Cappius HJ, Fluhr JW, Meinke MC (2015b) Perturbation factors in the clinical handling of a fiber-coupled Raman probe for cutaneous in vivo diagnostic Raman spectroscopy. Appl Spectrosc 69(2):243–256CrossRefPubMed

Schleusener J, Gluszczynska P, Reble C, Gersonde I, Helfmann J, Fluhr JW, Lademann J, Röwert-Huber J, Meinke MC (2015c) In vivo study for the discrimination of cancerous and normal skin using fiber probe based Raman spectroscopy. Exp Dermatol 24:767–772CrossRefPubMed

Schleusener J, Reble C, Helfmann J, Gersonde I, Cappius HJ, Glanert M, Fluhr JW, Meinke MC (2014) Design and technical evaluation of fibre-coupled Raman probes for the image-guided discrimination of cancerous skin. Meas Sci Technol 25(3) doi:10.1088/0957-0233/25/3/035701

Schroeder P, Lademann J, Darvin ME, Stege H, Marks C, Bruhnke S, Krutmann J (2008) Infrared radiation-induced matrix metalloproteinase in human skin: implications for protection. J Invest Dermatol 128(10):2491–2497CrossRefPubMed

Shah PP, Desai PR, Channer D, Singh M (2012) Enhanced skin permeation using polyarginine modified nanostructured lipid carriers. J Control Release 161(3):735–745PubMedCentralCrossRefPubMed

Shim MG, Wilson BC, Marple E, Wach M (1999) Study of fiber-optic probes for in vivo medical Raman spectroscopy. Appl Spectrosc 53(6):619–627CrossRef

Wang WZJ, Short M, Zeng H (2015) Real-time in vivo cancer diagnosis using raman spectroscopy. J Biophotonics 8:527–545CrossRefPubMed

Weigmann HJ, Jacobi U, Antoniou C, Tsikrikas GN, Wendel V, Rapp C, Gers-Barlag H, Sterry W, Lademann J (2005) Determination of penetration profiles of topically applied substances by means of tape stripping and optical spectroscopy: UV filter substance in sunscreens. J Biomed Opt 10(1):14009CrossRefPubMed

Zastrow L, Doucet O, Ferrero L, Groth N, Klein F, Kockott D, Lademann J (2015) Free radical threshold value – a new universal body constant. Skin Pharmacol Physiol 28:264–268CrossRefPubMed

Zastrow L, Groth N, Klein F, Kockott D, Lademann J, Renneberg R, Ferrero L (2009) The missing link – light-induced (280-1,600 nm) free radical formation in human skin. Skin Pharmacol Physiol 22(1):31–44CrossRefPubMed

Zhu Y, Choe CS, Ahlberg S, Meinke MC, Alexiev U, Lademann J, Darvin ME (2015) Penetration of silver nanoparticles into porcine skin ex vivo using fluorescence lifetime imagingmicroscopy, Raman microscopy, and surface-enhanced Raman scattering microscopy. J Biomed Optics 20(5):051006CrossRef

Titel: Raman-Spektroskopie in der Dermatologie
verfasst von: Jürgen Lademann, Prof. Dr. med.
Martina C. Meinke, PD Dr. rer. nat.
Alexa Patzelt, Dr. med.
Maxim E. Darvin, Dr. rer. med.
Verlag: Springer Berlin Heidelberg
Buch: Nichtinvasive physikalische Diagnostik in der Dermatologie
Print ISBN: 978-3-662-46388-8

Electronic ISBN: 978-3-662-46389-5

Copyright-Jahr: 2016
DOI: https://doi.org/10.1007/978-3-662-46389-5_8

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8. Raman-Spektroskopie in der Dermatologie

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Leitlinien kompakt für die Dermatologie

Neu im Fachgebiet Dermatologie

Auf diese Krankheiten bei Geflüchteten sollten Sie vorbereitet sein

Kein Abstrich bei chronischen Wunden ohne Entzündungszeichen!

„Nur wer sich gut aufgehoben fühlt, kann auch für Patientensicherheit sorgen“

Vitiligo: Prävalenz steigt und viele Erkrankte werden spät behandelt

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