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Neuroscience Bulletin
Erschienen in:

01.06.2019 | METHOD

Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus

verfasst von: Fan Jia, Xutao Zhu, Pei Lv, Liang Hu, Qing Liu, Sen Jin, Fuqiang Xu

Erschienen in: Neuroscience Bulletin | Ausgabe 3/2019

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Abstract

Sparse labeling of neurons contributes to uncovering their morphology, and rapid expression of a fluorescent protein reduces the experiment range. To achieve the goal of rapid and sparse labeling of neurons in vivo, we established a rapid method for depicting the fine structure of neurons at 24 h post-infection based on a mutant virus-like particle of Semliki Forest virus. Approximately 0.014 fluorescent focus-forming units of the mutant virus-like particle transferred enhanced green fluorescent protein into neurons in vivo, and its affinity for neurons in vivo was stronger than for neurons in vitro and BHK21 (baby hamster kidney) cells. Collectively, the mutant virus-like particle provides a robust and convenient way to reveal the fine structure of neurons and is expected to be a helper virus for combining with other tools to determine their connectivity. Our work adds a new tool to the approaches for rapid and sparse labeling of neurons in vivo.
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Metadaten
Titel
Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus
verfasst von
Fan Jia
Xutao Zhu
Pei Lv
Liang Hu
Qing Liu
Sen Jin
Fuqiang Xu
Publikationsdatum
01.06.2019
Verlag
Springer Singapore
Erschienen in
Neuroscience Bulletin / Ausgabe 3/2019
Print ISSN: 1673-7067
Elektronische ISSN: 1995-8218
DOI
https://doi.org/10.1007/s12264-019-00362-z

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