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02.05.2017 | Short Communication | Ausgabe 2/2018

International Journal of Legal Medicine 2/2018

Rapidly mutating Y-STR analyses of compromised forensic samples

Zeitschrift:
International Journal of Legal Medicine > Ausgabe 2/2018
Autoren:
Rashed Alghafri, Irena Zupanič Pajnič, Tomaž Zupanc, Jože Balažic, Pankaj Shrivastava
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s00414-017-1600-z) contains supplementary material, which is available to authorized users.
Highlights
- The ability to amplify RM Y-STRs from compromised evidence is crucial for investigations in some of forensic applications
- Seventy-year-old skeletons were used as a model for compromised forensic samples
- RM-Yplex assay produced haplotypes from all analysed WWII bone and tooth samples
- Fifty-six percent full profiles and 44% partial profiles were obtained
- Drop-outs appeared correlated with low DNA quantities and degradation of aged DNA
- At least half of the RM Y-STRs were amplified even from the most compromised samples analysed
Rashed Alghafri and Irena Zupanič Pajnič contributed equally to this work.

Abstract

Rapidly mutating Y-chromosomal short tandem repeats (RM Y-STRs) were identified to improve differentiation of unrelated males and also to enable separating closely and distantly related males in human identity testing in forensic and other applications. RM-Yplex assay was developed as a single multiplex that is capable of simultaneously amplifying all currently known RM Y-STRs, and reproducibility and sensitivity testing were performed on reference samples. Additional analyses are necessary to test its suitability for analysing compromised forensic samples. For this purpose, we applied the RM-Yplex assay to approximately 70-year-old skeletons that were used as a model for poorly preserved, challenging forensic samples. We analysed 57 male skeletal remains (bones and teeth) from 55 skeletons excavated from the Second World War (WWII) mass graves in Slovenia. The RM-Yplex typing was successful in all 57 samples; there were 56% full profiles obtained, and in partial profiles, up to 7 locus drop-outs were observed and they appeared correlated with low DNA quantities and degradation of DNA obtained from WWII bone and tooth samples. The longest loci, DYS403S1b, DYS547, DYS627 and DYS526b, were the most often dropped-out RM Y-STRs. In spite of high frequency of drop-out events, the RM-Yplex typing was successful in all WWII samples, showing the possibility of successful amplification of at least half of the RM Y-STRs even from the most compromised samples analysed.

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