Introduction
Materials and methods
Detection of HBsAg in HCC and matched non-cancerous tissues by IHC
Detection of HBV cccDNA by ddPCR
Serum hepatitis B surface antigen (HBsAg) assay
Serum alpha-fetoprotein (AFP) assay
Statistical analysis
Results
Correlation between serum HBsAg and intrahepatic HBsAg
Patients (n = 88) |
n (%) |
---|---|
Age (years) | |
< 50 | 25 (28.4) |
≥ 50 | 63 (71.6) |
Sex | |
Male | 75 (85.23) |
Female | 13 (14.77) |
Serum HBsAg | |
Positive | 56 (63.64) |
Negative | 32 (36.36) |
Serum AFP | |
< 500 ng/ml | 62 (70.46) |
≥ 500 ng/ml | 24 (27.27) |
Unknown | 2 (2.27) |
Tumor size | |
< 5 cm | 42 (47.73) |
≥ 5 cm | 46 (52.27) |
TP53 expressions | |
Negative | 50 (56.82) |
Positive | 38 (43.18) |
Ki-67 expression | |
< 10% | 45 (51.14) |
≥ 10% | 43 (48.86) |
(A) | Intrahepatic HBsAg in matched non-cancerous tissues | ||
---|---|---|---|
Number positive (%) | Number negative (%) | ||
Serum HBsAg | |||
Positive | 41 (73.2) | 15 (26.8) |
p < 0.0001 |
Negative | 6 (18.7) | 26 (81.3) |
(B) | Intrahepatic HBsAg in HCC tissues | ||
---|---|---|---|
Number positive (%) | Number negative (%) | ||
Serum HBsAg | |||
Positive | 6 (10.7) | 50 (89.3) |
p = 0.415 |
Negative | 1 (3.1) | 31 (96.9) |
Correlation between serum HBsAg and intrahepatic HBV cccDNA
Patients (n = 30) |
n (%) |
---|---|
Age (years) | |
< 50 | 13 (43.33) |
≥ 50 | 17 (56.67) |
Sex | |
Male | 26 (86.67) |
Female | 4 (13.33) |
Serum HBsAg | |
Positive | 18 (60.0) |
Negative | 12 (40.0) |
Serum AFP | |
< 500 ng/ml | 19 (63.33) |
≥ 500 ng/ml | 8 (26.67) |
Unknown | 3 (10.0) |
Tumor size | |
< 5 cm | 12 (40.0) |
≥ 5 cm | 18 (60.0) |
TP53 expressions | |
Negative | 16 (53.33) |
Positive | 14 (46.67) |
Ki-67 expression | |
< 10% | 8 (26.67) |
≥ 10% | 22 (73.33) |
(A) | HBV cccDNA in matched non-cancerous tissues | ||
---|---|---|---|
Number positive (%) | Number negative (%) | ||
Serum HBsAg | |||
Positive | 12 (66.7) | 6 (33.3) |
p = 0.007 |
Negative | 2 (16.7) | 10 (83.3) |
(B) | HBV cccDNA in HCC tissues | ||
---|---|---|---|
Number positive (%) | Number negative (%) | ||
Serum HBsAg | |||
Positive | 1 (5.6) | 17 (94.4) |
p = 1.0 |
Negative | 0 (0) | 12 (100) |
Correlation between intrahepatic HBsAg and intrahepatic HBV cccDNA
(A) | HBV cccDNA in matched non-cancerous tissues | ||
---|---|---|---|
Number positive (%) | Number negative (%) | ||
Intrahepatic HBsAg | |||
Positive | 11 (73.3) | 4 (26.7) | |
Negative | 3 (20.0) | 12 (80.0) |
p = 0.003 |
(B) | HBV cccDNA in HCC tissues | ||
---|---|---|---|
Number positive (%) | Number negative (%) | ||
Intrahepatic HBsAg | |||
Positive | 1 (100) | 0 (0) | |
Negative | 0 (0) | 29 (100) |
p = 0.033 |