Background
Methods
Subjects
Study design
Pulmonary function, anthropometric measurements and body composition
Muscle biopsy
Fiber typing
Satellite cells and myonuclei staining
Satellite cell isolation and culture
Proliferation assay
MRFs expression
Differentiation assay
Statistical analysis
Results
Muscle homogenates
Subject’s characteristics
Characteristics | Control (n = 9) | COPD > 70 cm2(n = 11) | COPD < 70 cm2(n = 6) |
---|---|---|---|
Age (years)
| 63 ± 2.3 | 64 ± 2.3 | 70 ± 2.4 |
BMI (kg/m
2
)
| 29.1 ± 2.4 | 28.1 ± 2.0 | 21.7 ± 2.6b |
FEV
1
, L
| 3.3 ± 0.2 | 1.0 ± 0.2a | 0.8 ± 0.1a |
FEV
1,
% predicted
| 106.3 ± 4.1 | 36.0 ± 4.9a | 26.0 ± 3.8a |
FEV
1
/FVC, %
| 76.0 ± 1.6 | 36.1 ± 1.8a | 29.0 ± 2.6b |
TLC, % predicted
| 98.6 ± 6.5 | 121.6 ± 6.6a | 125.7 ± 4.8a |
RV, % predicted
| 90.1 ± 15.3 | 173.2 ± 22.8a | 195.5 ± 22.9a |
DL
CO
, % predicted
| 96.9 ± 3.2 | 57.4 ± 6.7a | 40.0 ± 2.8b |
MTCSA (cm
2
)
| 120.3 ± 3.4 | 86.3 ± 4.2a | 60.8 ± 3.6b |
Type I fiber
| |||
Distribution, %
| 51.5 ± 5.7 | 34.7 ± 9.3a | 34.9 ± 3.5a |
CSA, (μm
2
)
| 5170 ± 471 | 4711 ± 340 | 4883 ± 607 |
Type II fiber
| |||
Distribution, %
| 44.1 ± 5.7 | 65.4 ± 9.3a | 65.2 ± 3.5a |
CSA, (μm
2
)
| 4216 ± 358 | 4359 ± 540 | 4096 ± 480 |
Satellite cells and central nuclei quantification
Expression of myogenic regulatory factors
Satellite cell analyses
Subject’s characteristics
Characteristics | Control (n = 7) | COPD (n = 8) |
---|---|---|
Age
| 63 ± 2.3 | 66 ± 2.3 |
BMI (kg/m
2
)
| 29.1 ± 2.4 | 28.8 ± 2.0 |
FEV
1
, L
| 3.3 ± 0.2 | 1.0 ± 0.1a |
FEV
1,
% predicted
| 106.3 ± 4.1 | 37.0 ± 4.0a |
FEV
1
/FVC, %
| 76 ± 1.6 | 36.5 ± 1.1a |
TLC, % predicted
| 98.6 ± 6.5 | 118.0 ± 5.5 |
RV, % predicted
| 90.1 ± 15.3 | 163.5 ± 15.9a |
DL
CO
, % predicted
| 96.9 ± 3.2 | 53.3 ± 5.8a |
MTCSA (cm
2
)
| 120.3 ± 3.4 | 82.3 ± 6.5a |
Satellite cells (Pax7+/100 fibers)
| 2.6 ± 0.4 | 1.7 ± 0.3a |
Central nuclei (count/100 fibers)
| 4.0 ± 0.8 | 6.5 ± 1.0a |