Resistance training-induced changes in muscle proteolysis and extracellular matrix remodeling biomarkers in the untrained and trained states
- 23.04.2024
- Original Article
Erschienen in:
- Verfasst von
- Maíra C. Scarpelli
- João G. A. Bergamasco
- Joshua S. Godwin
- Paulo H. C. Mesquita
- Talisson S. Chaves
- Deivid G. Silva
- Diego Bittencourt
- Nathalia F. Dias
- Ricardo A. Medalha Junior
- Paulo C. Carello Filho
- Vitor Angleri
- Luiz A. R. Costa
- Andreas N. Kavazis
- Carlos Ugrinowitsch
- Michael D. Roberts
- Cleiton A. Libardi
- Erschienen in
- European Journal of Applied Physiology | Ausgabe 9/2024
Abstract
Purpose
Resistance training (RT) induces muscle growth at varying rates across RT phases, and evidence suggests that the muscle-molecular responses to training bouts become refined or attenuated in the trained state. This study examined how proteolysis-related biomarkers and extracellular matrix (ECM) remodeling factors respond to a bout of RT in the untrained (UT) and trained (T) state.
Methods
Participants (19 women and 19 men) underwent 10 weeks of RT. Biopsies of vastus lateralis were collected before and after (24 h) the first (UT) and last (T) sessions. Vastus lateralis cross-sectional area (CSA) was assessed before and after the experimental period.
Results
There were increases in muscle and type II fiber CSAs. In both the UT and T states, calpain activity was upregulated and calpain-1/-2 protein expression was downregulated from Pre to 24 h. Calpain-2 was higher in the T state. Proteasome activity and 20S proteasome protein expression were upregulated from Pre to 24 h in both the UT and T. However, proteasome activity levels were lower in the T state. The expression of poly-ubiquitinated proteins was unchanged. MMP activity was downregulated, and MMP-9 protein expression was elevated from Pre to 24 h in UT and T. Although MMP-14 protein expression was acutely unchanged, this marker was lower in T state. TIMP-1 protein levels were reduced Pre to 24 h in UT and T, while TIMP-2 protein levels were unchanged.
Conclusion
Our results are the first to show that RT does not attenuate the acute-induced response of proteolysis and ECM remodeling-related biomarkers.
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- Titel
- Resistance training-induced changes in muscle proteolysis and extracellular matrix remodeling biomarkers in the untrained and trained states
- Verfasst von
-
Maíra C. Scarpelli
João G. A. Bergamasco
Joshua S. Godwin
Paulo H. C. Mesquita
Talisson S. Chaves
Deivid G. Silva
Diego Bittencourt
Nathalia F. Dias
Ricardo A. Medalha Junior
Paulo C. Carello Filho
Vitor Angleri
Luiz A. R. Costa
Andreas N. Kavazis
Carlos Ugrinowitsch
Michael D. Roberts
Cleiton A. Libardi
- Publikationsdatum
- 23.04.2024
- Verlag
- Springer Berlin Heidelberg
- Erschienen in
-
European Journal of Applied Physiology / Ausgabe 9/2024
Print ISSN: 1439-6319
Elektronische ISSN: 1439-6327 - DOI
- https://doi.org/10.1007/s00421-024-05484-5
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