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Erschienen in: Journal of Parasitic Diseases 3/2016

24.09.2014 | Short Communication

Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates

verfasst von: K. P. Shyma, S. K. Gupta, J. P. Gupta, Ajit Singh, S. S. Chaudhari, Veer Singh

Erschienen in: Journal of Parasitic Diseases | Ausgabe 3/2016

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Abstract

The differences or similarities among different isolates of Trypanosoma evansi through endonuclease profile was identified in the present study. The repetitive nuclear DNA of T. evansi isolated from infected cattle, buffalo and equine blood was initially amplified by PCR using specific primers. A panel of restriction enzymes, EcoRI, Eco91l, HindIII and PstI were for complete digestion of PCR products. Agarose gel electrophoresis of digested product did not show cleavage fragments and only single DNA band of the original size was visible in the ethidium bromide stained agarose gel. This indicated that the 227 bp PCR product from repetitive sequence had no site-specific cleavage sites for the REs used in this study. No heterogeneity in the repetitive nuclear DNA restriction endonuclease profile among the different isolates was recorded.
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Metadaten
Titel
Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates
verfasst von
K. P. Shyma
S. K. Gupta
J. P. Gupta
Ajit Singh
S. S. Chaudhari
Veer Singh
Publikationsdatum
24.09.2014
Verlag
Springer India
Erschienen in
Journal of Parasitic Diseases / Ausgabe 3/2016
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI
https://doi.org/10.1007/s12639-014-0582-8

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