01.12.2013 | Original Paper | Ausgabe 12/2013
RETRACTED ARTICLE: siRNA-participated
chemotherapy: an efficient and specific therapeutic against gastric
Journal of Cancer Research and Clinical Oncology
- Donglei Zhou, Xun Jiang, Weixing Ding, Lijun Zheng, Lei Yang, Chengzhu Zheng, Liesheng Lu
This study aims to investigate the role of siRNA silencing fibroblast growth
factor receptor (FGFR) expression in promoting chemotherapy effect of gastric
cancer and to explore its mechanism.
Human gastric cancer cells MGC80-3 were divided into four groups: control
group, cisplatin group (2 μg/L), cisplatin (2 μg/L) + siRNA group and siRNA
group. The expressions of FGFR in four groups were detected by
immunofluorescence. The cell proliferation and apoptosis were detected by MTT
assay and flow cytometry. The protein expression levels of vascular endothelial
growth factor receptor (VEGFR), caspase-3 and Bax were detected by Western blot.
Further, animal model of gastric cancer was established and divided into four
groups as in vitro experiment. The expression of FGFR mRNA in tumor tissue was
detected by the real-time fluorescence quantitative polymerase chain reaction.
The size of tumor was measured to analyze the effects of treatment.
Histopathological detections were performed by hematoxylin and eosin staining
For in vitro experiment, significant decrease inFGFR expression, inhibition of
proliferation and promotion of apoptosis were observed in siRNA-treated cells,
so as cisplatin group. siRNA also resulted in the reduction of VEGFR and rise in
apoptosis-related protein (caspase-3). As for the experiment in vivo, siRNA also
suppressed the expression of FGFR and enhanced tumor shrink. Furthermore, the
co-administration of siRNA and cisplatin revealed a more excellent antitumor
effect than other therapies.
siRNA can effectively suppress FGFR expression and cell proliferation, but
promote apoptosis in vitro and also inhibit tumor growth and FGFR production in
vivo. siRNA-participated chemotherapy may provide an efficient therapeutic
approach to treat gastric cancer.