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Archives of Virology

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11.04.2016 | Brief Report

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid diagnosis of chilli veinal mottle virus

verfasst von: Amrita Banerjee, Somnath Roy, Susheel Kumar Sharma, Sudip Kumar Dutta, Satish Chandra, SV Ngachan

Erschienen in: Archives of Virology | Ausgabe 7/2016

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Abstract

Chilli veinal mottle virus (ChiVMV) causes significant economic loss to chilli cultivation in northeastern India, as well as in eastern Asia. In this study, we have developed a single-tube one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid, sensitive and specific diagnosis of ChiVMV. Amplification could be visualized after adding SYBR Green I (1000×) dye within 60 min under isothermal conditions at 63 °C, with a set of four primers designed based on the large nuclear inclusion protein (NIb) domain of ChiVMV (isolate KC-ML1). The RT-LAMP method was 100 times more sensitive than one-step reverse transcription polymerase chain reaction (RT-PCR), with a detection limit of 0.0001 ng of total RNA per reaction.

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Titel: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid diagnosis of chilli veinal mottle virus
verfasst von: Amrita Banerjee
Somnath Roy
Susheel Kumar Sharma
Sudip Kumar Dutta
Satish Chandra
SV Ngachan
Publikationsdatum: 11.04.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-2850-7

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Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid diagnosis of chilli veinal mottle virus

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