Skip to main content
main-content

01.12.2014 | Research article | Ausgabe 1/2014 Open Access

BMC Cardiovascular Disorders 1/2014

Rheumatic heart disease in Uganda: the association between MHC class II HLA DR alleles and disease: a case control study

Zeitschrift:
BMC Cardiovascular Disorders > Ausgabe 1/2014
Autoren:
Emmy Okello, Andrea Beaton, Charles K Mondo, Paul Kruszka, Noah Kiwanuka, Richard Odoi-Adome, Juergen Freers
Wichtige Hinweise

Competing interests

The authors declare that they have no competing interest.

Authors’ contribution

EO, CM designed the study, EO, PK and AB did the molecular analysis, EO, PK and NK did the statistical analysis. EO, PK, NK, CM, AB, AB, CM, RO, JF wrote the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Rheumatic heart disease (RHD), the only long term consequence of acute rheumatic fever, remains a leading cause of morbidity and mortality among young adults in Uganda. An inherited susceptibility to acute rheumatic fever centers around the major histocompatibility class II human leucocyte antigens. However, there is paucity of data from sub-Saharan Africa. This study compares the frequency of HLA class II DR alleles between RHD cases and normal controls in Uganda.

Methods

One hundred ninety-nine participants including 96 established RHD cases aged 5–60 years and 103 age and sex matched normal controls were recruited for participation. DNA was manually extracted from buffy coat samples and HLA analysis was performed. HLA-DR allelic frequency comparison between cases and controls were estimated using conditional logistic regression with 95% confidence intervals. P -values were corrected for multiple hypothesis testing.

Results

199 participants (103 female, 51.8%) completed the study. The mean (SD) age in years for cases and controls were 29.6 (10.2) and 29(18), respectively. After conditional logistic regression and multiple hypothesis testing, HLA-DR1was associated with a decreased risk of RHD (OR = 0.42, CI 0.21-085, P = 0.01, Corrected P value (PC) = 0.09,) while HLA-DR11 was associated with increased risk of RHD (OR = 3.31, CI 1.57-6.97, P = <0.001, Pc < 0.001). No other significant associations were found.

Conclusion

In this first study of HLA genetic susceptibility to RHD in Uganda, HLA- DR1 was more common in normal controls while HLA- DR11 was more common among RHD cases suggesting a disease susceptibility association. In future studies, high resolution HLA analysis and genome wide studies should be carried out to confirm this pattern.
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2014

BMC Cardiovascular Disorders 1/2014 Zur Ausgabe

Reviewer acknowledgement

Reviewer Acknowledgement 2013