The online version of this article (https://doi.org/10.1186/s12865-018-0240-5) contains supplementary material, which is available to authorized users.
The Haemophilus influenzae type b (Hib) conjugate vaccine has been widely used in children to prevent invasive Hib disease because of its strong immunogenicity and antibody response induction relative to the capsular polysaccharide (CPS) antigen. The data from vaccine studies suggest that the conjugate vaccine contains carrier proteins that enhance and/or regulate the antigen’s immunogenicity, but the mechanism of this enhancement remains unclear.
To explore the immunological role of the conjugate vaccine, we compared the immune responses and gene profiles of rhesus macaques after immunization with CPS, carrier protein tetanus toxoid (TT) or conjugate vaccine.
A distinct immune response was induced by the Hib conjugate vaccine but not by CPS or carrier protein TT. The genes that were dynamically regulated in conjunction with the macaque immune responses to the conjugate vaccine were investigated.
We propose that these genes are involved in the induction of specific immunity that is characterized by the appearance and maintenance of antibodies against Hib.
Additional file 1: Table S1. two monkeys in each group were immunized intramuscular injection. Table S2. The primers of eight genes for real-time RT-PCR. Figure S1 ELISA analysis of total antibody titers from immunized rhesus macaques. Figure S2. ELISA analysis of antibody from immunized rhesus macaques. Figure S3. qRT-PCR analysis of mRNA at various time points. Figure S4. qRT-PCR and Flow cytometry analysis of CD69 and ITK expression. Tables S1, S2, S3, S4, Figures S1, S2, S3 and S4. A remarkable immune response induced by Hib conjugate vaccine, in rhesus macaques, because of a result of the synergistic effects between the carrier TT and CPS antigen. (DOCX 1352 kb)12865_2018_240_MOESM1_ESM.docx
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- RNA gene profile variation in peripheral blood mononuclear cells from rhesus macaques immunized with Hib conjugate vaccine, Hib capsular polysaccharide and TT carrier protein
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