Background
Results
Variable levels of SIRT3 expression and its activity
Discovery of SIRT3 sequence variations
SIRT3 | ||||
---|---|---|---|---|
WT
|
V208I
|
P345R
|
H248Y
| |
NAD
+
V
max
(pmole/min/mg)
|
16.03 ± 0.02
|
12.14 ± 0.06
|
15.94 ± 0.14
|
1.08 ± 0.16
|
K
M
(mM) |
0.88 ± 0.01
|
1.07 ± 0.03
|
0.88 ± 0.07
|
N.D.
|
Catalytic efficiency (V
max
/K
M
)
|
18.21
|
11.35
|
18.11
|
N.D.
|
Peptide V
max
(pmole/min/mg)
|
17.5 ± 0.69
|
12.5 ± 0.03
|
17.32 ± 0.45
|
1.32 ± 0.11
|
K
M
(mM) |
147.3 ± 12.4
|
153.4 ± 17.5
|
146.8 ± 10.8
|
54.2 ± 14.0
|
Catalytic efficiency (V
max
/K
M
)
|
0.11
|
0.07
|
0.12
|
0.02
|