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Erschienen in: Comparative Clinical Pathology 2/2019

09.11.2018 | Original Article

Rumex alveollatus hydroalcoholic extract protects CCL4-induced hepatotoxicity in mice

verfasst von: Leila Naseri, Mozafar Khazaei, Elham Ghanbari, Mohsen Akbari Bazm

Erschienen in: Comparative Clinical Pathology | Ausgabe 2/2019

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Abstract

Some plants have high antioxidant properties that can improve the damage to the tissues of the body. Rumex alveollatus L. is proposed to contain flavonoids, a group of antioxidants that exert their protective effects against free radicals. Carbon tetrachloride (CCl4) is a factor that damages the liver. This study was carried out to explore the protective effects of Rumex alveollatus L. on the liver damage induced by CCl4 in mice. In this experimental study, 30 male mice (35 ± 4) were divided into five groups, each with six mice. The negative control group was administered 0.5-ml olive oil; positive control group was administered 2-ml/kg CCl4 plus olive oil at 1:1 ratio for 4 days, and the experimental groups were administered 2 ml/kg CCl4 and Rumex alveollatus L. extract at 150, 300, and 450 mg/kg for 4 days. All administrations were done intraperitoneally. Twenty days later, blood samples were taken from the heart, and liver serum enzymes (ALT, AST, and ALP) were measured. After sacrificing the samples, liver samples were fixed in formalin to prepare the histological sections. Then, factors such as liver volume, hepatocytes, sinusoids, central veins, portal vein, bile duct, and hepatic artery were measured. Rumex alveollatus L. extract (450 mg/kg) reduced the serum level of all three liver enzymes (p < 0.05). At this dose, the volume of sinusoids, hepatocytes, and bile duct decreased significantly (p < 0.05), but the volume of portal vein and central vein increased significantly (p < 0.05). Rumex alveollatus L. extract has protective effects against CCl4-induced damages in the liver.
Literatur
Zurück zum Zitat Aghel N, Rashidi I, Mombeini A (2010) Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice. Iran J Pharm Res 285–90 Aghel N, Rashidi I, Mombeini A (2010) Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice. Iran J Pharm Res 285–90
Zurück zum Zitat Amin A, Hamza AA (2005) Hepatoprotective effects of Hibiscus, Rosmarinus and Salvia on azathioprine-induced toxicity in rats. Life Sci 77:266–278CrossRefPubMed Amin A, Hamza AA (2005) Hepatoprotective effects of Hibiscus, Rosmarinus and Salvia on azathioprine-induced toxicity in rats. Life Sci 77:266–278CrossRefPubMed
Zurück zum Zitat Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99:191–203CrossRef Balasundram N, Sundram K, Samman S (2006) Phenolic compounds in plants and agri-industrial by-products: antioxidant activity, occurrence, and potential uses. Food Chem 99:191–203CrossRef
Zurück zum Zitat Cantwell SL (2001) Ferret, rabbit, and rodent anesthesia. Veterinary clinics of North America exotic animal practice 4:169–191CrossRefPubMed Cantwell SL (2001) Ferret, rabbit, and rodent anesthesia. Veterinary clinics of North America exotic animal practice 4:169–191CrossRefPubMed
Zurück zum Zitat Cetinkaya O, Siliğ Y, Cetinkaya S, Demirezer LO (2002) The effects of Rumex patientia extract on rat liver and erythrocyte antioxidant enzyme system. Die Pharmazie 57:487–488PubMed Cetinkaya O, Siliğ Y, Cetinkaya S, Demirezer LO (2002) The effects of Rumex patientia extract on rat liver and erythrocyte antioxidant enzyme system. Die Pharmazie 57:487–488PubMed
Zurück zum Zitat Chatila R (2000) Hepatotoxicity: the adverse effects of drugs and other chemicals on the liver. J Clin Gastroenterol 31:185CrossRef Chatila R (2000) Hepatotoxicity: the adverse effects of drugs and other chemicals on the liver. J Clin Gastroenterol 31:185CrossRef
Zurück zum Zitat Feyzmahdavia M, Khazaeia M, Gholaminb B, Abbasabadia Z (2017) Protective effect of Trogopogon Graminifolius hydroalcoholic extract against acute ethanol induced liver damages in rat. JRPS 6:161–166 Feyzmahdavia M, Khazaeia M, Gholaminb B, Abbasabadia Z (2017) Protective effect of Trogopogon Graminifolius hydroalcoholic extract against acute ethanol induced liver damages in rat. JRPS 6:161–166
Zurück zum Zitat Ganaie MA, Khan TH, Siddiqui NA, Ansari MN (2015) Ameliorative effect of methanol extract of Rumex vesicarius on CCl4-induced liver damage in Wistar albino rats. Pharm Biol 53:1163–1167CrossRefPubMed Ganaie MA, Khan TH, Siddiqui NA, Ansari MN (2015) Ameliorative effect of methanol extract of Rumex vesicarius on CCl4-induced liver damage in Wistar albino rats. Pharm Biol 53:1163–1167CrossRefPubMed
Zurück zum Zitat Ghanbari E, Khazaei M, Yousefzaei F (2017) The restorative effect of prosopis farcta on fertility parameters and antioxidant status in diabetic rats. JBUMS 19:53–60 Ghanbari E, Khazaei M, Yousefzaei F (2017) The restorative effect of prosopis farcta on fertility parameters and antioxidant status in diabetic rats. JBUMS 19:53–60
Zurück zum Zitat Goodarzi N, Doorgard E, Pournaghi P (2018) The ameliorative potential of Sophora alopecuroides essential oil on CCl4-induced hepatotoxicity in mice; a stereological study. RJP 5:47–54 Goodarzi N, Doorgard E, Pournaghi P (2018) The ameliorative potential of Sophora alopecuroides essential oil on CCl4-induced hepatotoxicity in mice; a stereological study. RJP 5:47–54
Zurück zum Zitat Gundersen HJ, Jensen EB (1987) The efficiency of systematic sampling in stereology and its prediction. J Microsc Ultrastruct 147:229–263CrossRef Gundersen HJ, Jensen EB (1987) The efficiency of systematic sampling in stereology and its prediction. J Microsc Ultrastruct 147:229–263CrossRef
Zurück zum Zitat Gundersen HJ, BENDTSEN TF, KORBO L, MARCUSSEN N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Apmis 96:379–394CrossRefPubMed Gundersen HJ, BENDTSEN TF, KORBO L, MARCUSSEN N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. Apmis 96:379–394CrossRefPubMed
Zurück zum Zitat Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y (2003) Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 23:1719–1726CrossRef Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y (2003) Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 23:1719–1726CrossRef
Zurück zum Zitat Halliwell B (1997) Antioxidants in disease mechanisms and therapy. Adv Pharmacol Sci 38:3–17CrossRef Halliwell B (1997) Antioxidants in disease mechanisms and therapy. Adv Pharmacol Sci 38:3–17CrossRef
Zurück zum Zitat Jaeschke H, Gores GJ, Cederbaum AI, Hinson JA, Pessayre D, Lemasters JJ (2002) Mechanisms of hepatotoxicity. Toxicol Sci 65:166–176CrossRefPubMed Jaeschke H, Gores GJ, Cederbaum AI, Hinson JA, Pessayre D, Lemasters JJ (2002) Mechanisms of hepatotoxicity. Toxicol Sci 65:166–176CrossRefPubMed
Zurück zum Zitat Jimoh FO, Adedapo AA, Aliero AA, Afolayan AJ (2008) Polyphenolic contents and biological activities of Rumex ecklonianus. Pharm Biol 46:333–340CrossRef Jimoh FO, Adedapo AA, Aliero AA, Afolayan AJ (2008) Polyphenolic contents and biological activities of Rumex ecklonianus. Pharm Biol 46:333–340CrossRef
Zurück zum Zitat Kasote DM, Katyare SS, Hegde MV, Bae H (2015) Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci 11:982–991CrossRefPubMedPubMedCentral Kasote DM, Katyare SS, Hegde MV, Bae H (2015) Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci 11:982–991CrossRefPubMedPubMedCentral
Zurück zum Zitat Khazaei M, Roshankhah S, Ghorbani R, Chobsaz F (2011) Sildenafil effect on nitric oxide secretion by normal human endometrial epithelial cells cultured in vitro. Int J Fertil & Steril 5:142 Khazaei M, Roshankhah S, Ghorbani R, Chobsaz F (2011) Sildenafil effect on nitric oxide secretion by normal human endometrial epithelial cells cultured in vitro. Int J Fertil & Steril 5:142
Zurück zum Zitat Korkorian N, Mohammadi-Sichani M (2017) Anti-quorum sensing and antibacterial activity of Rumex alveolatus. Zahedan J Res Med Sci 19(11) Korkorian N, Mohammadi-Sichani M (2017) Anti-quorum sensing and antibacterial activity of Rumex alveolatus. Zahedan J Res Med Sci 19(11)
Zurück zum Zitat Latha S, Chaudhary S, Ray RS (2017) Hydroalcoholic extract of Stevia rebaudiana bert. leaves and stevioside ameliorates lipopolysaccharide induced acute liver injury in rats. Biomed Pharmacother 95:1040–1050CrossRef Latha S, Chaudhary S, Ray RS (2017) Hydroalcoholic extract of Stevia rebaudiana bert. leaves and stevioside ameliorates lipopolysaccharide induced acute liver injury in rats. Biomed Pharmacother 95:1040–1050CrossRef
Zurück zum Zitat Lee SS, Kim DH, Yim DS, Lee SY (2007) Anti-inflammatory, analgesic and hepatoprotective effect of semen of Rumex crispus. Korean J Physiol Pharmacol Lee SS, Kim DH, Yim DS, Lee SY (2007) Anti-inflammatory, analgesic and hepatoprotective effect of semen of Rumex crispus. Korean J Physiol Pharmacol
Zurück zum Zitat Mirhosseini M (2017) Medicinal plants with anti-poisoning toxicity of carbon tetrachloride: an overview of the most important medicinal plants native to Iran with anti-carbon tetrachloride toxicity. Journal of Global Pharma Technology 17–20 Mirhosseini M (2017) Medicinal plants with anti-poisoning toxicity of carbon tetrachloride: an overview of the most important medicinal plants native to Iran with anti-carbon tetrachloride toxicity. Journal of Global Pharma Technology 17–20
Zurück zum Zitat Nejad ZS, Bamakan AM, Sharfififar F, Mokhtari S, Iranpour M (2015) Evaluation of the hepatoprotective effects of rumex acetosella on the carbon tetrachloride-induced hepatotoxicity in rats. Avicenna J Phytomed 5:134–135CrossRef Nejad ZS, Bamakan AM, Sharfififar F, Mokhtari S, Iranpour M (2015) Evaluation of the hepatoprotective effects of rumex acetosella on the carbon tetrachloride-induced hepatotoxicity in rats. Avicenna J Phytomed 5:134–135CrossRef
Zurück zum Zitat NK AT, Londonkar RL, Nayaka HB (2015) Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius. L. Biol Res 48:19CrossRef NK AT, Londonkar RL, Nayaka HB (2015) Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius. L. Biol Res 48:19CrossRef
Zurück zum Zitat Nyengaard JR (1999) Stereologic methods and their application in kidney research. Clin J Am Soc Nephrol 10:1100–1123 Nyengaard JR (1999) Stereologic methods and their application in kidney research. Clin J Am Soc Nephrol 10:1100–1123
Zurück zum Zitat Rajkapoor B, Anandan R, Jayakar B (2002) Hepatoprotective activity of Nigella sativa Linn. Indian Drugs 39:398–399 Rajkapoor B, Anandan R, Jayakar B (2002) Hepatoprotective activity of Nigella sativa Linn. Indian Drugs 39:398–399
Zurück zum Zitat Saleem M, Ahmed B, Karim M, Ahmed S, Ahmad M, Qadir MI (2014) Hepatoprotective effect of aqeous methanolic extract of Rumex dentatus in paracetamol induced hepatotoxicity in mice. Bangladesh J Pharmacol 9:284–289 Saleem M, Ahmed B, Karim M, Ahmed S, Ahmad M, Qadir MI (2014) Hepatoprotective effect of aqeous methanolic extract of Rumex dentatus in paracetamol induced hepatotoxicity in mice. Bangladesh J Pharmacol 9:284–289
Zurück zum Zitat Saleh NA, El-Hadidi MN, Arafa RF (1993) Flavonoids and anthraquinones of some Egyptian Rumex species (Polygonaceae). Biochem Syst Ecol 21:301–303CrossRef Saleh NA, El-Hadidi MN, Arafa RF (1993) Flavonoids and anthraquinones of some Egyptian Rumex species (Polygonaceae). Biochem Syst Ecol 21:301–303CrossRef
Zurück zum Zitat Sharma B, Kumar SU (2010) Hepatoprotective activity of some indigenous plants. Int J Pharmtech Res 2:568–572 Sharma B, Kumar SU (2010) Hepatoprotective activity of some indigenous plants. Int J Pharmtech Res 2:568–572
Zurück zum Zitat Silig Y, Cetinkaya O, Demirezer LO (2004) Effects of Rumex patientia L extract on some drug-metabolizing enzymes in rat liver, 41: 45–47 Silig Y, Cetinkaya O, Demirezer LO (2004) Effects of Rumex patientia L extract on some drug-metabolizing enzymes in rat liver, 41: 45–47
Zurück zum Zitat Tavafi M, Ahmadvand H, Tamjidipoor A, Delfan B, Khalatbari AR (2011) Satureja khozestanica essential oil ameliorates progression of diabetic nephropathy in uninephrectomized diabetic rats. Tissue Cell 43:45–51CrossRefPubMed Tavafi M, Ahmadvand H, Tamjidipoor A, Delfan B, Khalatbari AR (2011) Satureja khozestanica essential oil ameliorates progression of diabetic nephropathy in uninephrectomized diabetic rats. Tissue Cell 43:45–51CrossRefPubMed
Zurück zum Zitat Ubhenin AE, Igbe I, Adamude FA, Falodun A (2016) Hepatoprotective effects of ethanol extract of Caesalpinia bonduc against carbon tetrachloride induced hepatotoxicity in albino rats. J Appl Sci Environ Manag 20:396–401 Ubhenin AE, Igbe I, Adamude FA, Falodun A (2016) Hepatoprotective effects of ethanol extract of Caesalpinia bonduc against carbon tetrachloride induced hepatotoxicity in albino rats. J Appl Sci Environ Manag 20:396–401
Zurück zum Zitat Vasas A, Orbán-Gyapai O, Hohmann J (2015) The genus Rumex: review of traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 175:198–228CrossRefPubMed Vasas A, Orbán-Gyapai O, Hohmann J (2015) The genus Rumex: review of traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 175:198–228CrossRefPubMed
Zurück zum Zitat Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018a) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl 4-induced hepatic injury in mice. Comp Clin Pathol, 1–7 Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018a) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl 4-induced hepatic injury in mice. Comp Clin Pathol, 1–7
Zurück zum Zitat Zangeneh MM, Zangeneh A, Almasi M, Tahvilian R, Hosseini F, Moradi R (2018b) A comparative study of hepatoprotective effect of Inula britannica L aqueous extract and glibenclamide in streptozotocin-induced diabetic mice. Comp Clin Pathol, 1–9 Zangeneh MM, Zangeneh A, Almasi M, Tahvilian R, Hosseini F, Moradi R (2018b) A comparative study of hepatoprotective effect of Inula britannica L aqueous extract and glibenclamide in streptozotocin-induced diabetic mice. Comp Clin Pathol, 1–9
Metadaten
Titel
Rumex alveollatus hydroalcoholic extract protects CCL4-induced hepatotoxicity in mice
verfasst von
Leila Naseri
Mozafar Khazaei
Elham Ghanbari
Mohsen Akbari Bazm
Publikationsdatum
09.11.2018
Verlag
Springer London
Erschienen in
Comparative Clinical Pathology / Ausgabe 2/2019
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-018-2846-7

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