The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
RMA: initiated and conceptualised the protocol; patient recruitment, data collection, patient sampling, drafting of the manuscript, analysis of the data, statistical correlation, editing and writing of the final version. HIA: was responsible for the final editing, language review and statistical correlation, critical scientific revision RHS: carried out the extraction and amplification steps of the Real-time PCR, ELISA assays and Latex agglutination tests of the samples collected, writing of the technical details performed and interpretation of the respective results. AMS: participated in the study design,data collection and interpretation, final editing of the final manuscript and critical revision of the scientific material. NAS: shared in the study design, drafting of the manuscript, data interpretation, preliminary editing and submission processes. HG: patient recruitment, sampling of the control group, data collection and analysis. AL: patient recruitment, data collection and statistical interpretation, statistical analysis of the control group. MAM: patient sampling for the control group,data collection, interpretation and and statistical correlation. MAB: collection of scientific material, critical revision of content, data interpretation and analysis. ANH: data interpretation and analysis, revision of scientific content and final editing. AM: participated in data collection, statistical analysis of data and interpretation of results. MAF: initiated and conceptualised the protocol; patient recruitment, data collection, study coordination, drafting of the manuscript and writing of the final version and submission process. All authors read and approved the final manuscript.
Details of ethics approval: approved by the Scientific and Ethical Committee of Obstetrics & Gynecology department- Cairo University.
To study the prevalence of Chlamydia infection in women with primary and secondary unexplained infertility using ELISA technique for antibody detection and real time, fully automated PCR for antigen detection and to explore its association with circulating antisperm antibodies (ASA).
A total of 50 women with unexplained infertility enrolled in this case control study and a control group of 44 infertile women with a known cause of infertility. Endocervical specimens were collected for Chlamydia antigen detection using PCR and serum samples for antibodies detection. Circulating anti-sperm antibodies were detected using sperm antibody Latex Agglutination tests.
The overall prevalence of Chlamydial infection in unexplained infertility cases as detected by both ELISA and PCR was 40 % (20/50). The prevalence of current Chlamydial genital infection as detected by real-time PCR was only 6.0 % (3/50); two of which were also IgM positive. Prevalence of ASA was 6.0 % (3/50); all were sero-negative for anti-C.trachomatis IgM and were PCR negative.
The incidence of Chlamydial infection in Egyptian patients with unexplained infertility is relatively high. In the setting of fertility investigations; screening for anti. C.trachomatis antibodies using ELISA, and treatment of positive cases should be considered. The presence of circulating ASA does not correlate with the presence of old or current Chlamydia infection in women with unexplained infertility.
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Screening for Chlamydia trachomatis in Egyptian women with unexplained infertility, comparing real-time PCR techniques to standard serology tests: case control study
Chlamydia trachomatis and unexplained infertility
Rana M. A. Abdella
Hatem I. Abdelmoaty
Rasha H. Elsherif
Ahmed Mahmoud Sayed
Nadine Alaa Sherif
Hisham M. Gouda
Ahmed El Lithy
Ahmed Naguib Hosni
- BioMed Central
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