Background
Rheumatoid Arthritis (RA) is a chronic, autoimmune inflammatory disease affecting 0.5–1% of the population, manifesting with joint swelling, cartilage destruction and bone erosion. These clinical characteristics contribute towards joint pain and impaired physical function symptomatic of the disease [
1‐
3]. The heightened inflammatory-load seen in RA may also contribute to the increased risk of cardiovascular disease (CVD) and associated morbidity and mortality [
4‐
10].
It is well evidenced that higher levels of physical activity (PA) associate with improvement in cardiovascular health, systemic inflammation and physical function in RA [
11‐
20]. This relationship is reported to occur in a ‘dose-response’ manner; with moderate-to-vigorous intensity physical activity [MVPA, i.e., ≥ 3 metabolic equivalents (METS)] demonstrated to be particularly beneficial for RA outcomes [
16‐
20]. However, recent studies conducted among older adults and non-RA clinical populations, have underlined the role of engagement in light intensity physical activity (LPA, i.e., 1.6 – 2.9 METS) for reducing cardiovascular risk, improving metabolic health and attenuating functional disability [
21‐
28]. Thus, engagement in LPA may also be relevant to improving pertinent RA outcomes.
Still, despite evidence highlighting the positive consequences of PA engagement for RA outcomes, people living with RA remain physically inactive, and are often referred to as “sedentary” [
29‐
31]. However, it is important to recognise that whilst both sedentary behaviour and physical inactivity represent the ‘non-exercise’ part of the physical activity continuum, they are distinct constructs, and can be operationalised as such [
11,
32,
33]. Specifically, physically inactivity refers to failure to meet the recommended 150 min of MVPA per week [
33,
34], where sedentary behaviour is defined as any waking behaviour resulting in energy expenditure ≤ 1.5 METS whilst sitting or lying [
32,
35].
Sedentary behaviour is demonstrated to be adversely linked to several health outcomes which are relevant to RA. For example, studies have revealed time spent sedentary to hold deleterious consequences for cardiovascular and cardio-metabolic health, as well as functional ability for both healthy adults and patient groups [
21,
36‐
40]. Moreover, the way sedentary time is accumulated is reported to hold implications for CVD risk and physical function. Specifically, the number and length of uninterrupted sedentary periods (i.e., sedentary bouts), and the frequency of interruptions in sedentary time with standing and/or LPA (i.e., sedentary breaks) are reported to be associated with markers of cardio-metabolic and cardiovascular health, and physical dysfunction in older cohorts [
36,
37,
41‐
48]. However, research is yet to examine the implications of objectively measured sedentary behaviour patterns and LPA for risk of CVD (and other relevant health outcomes) in RA [
49].
The primary aim of this study was therefore to examine the relationships between objectively assessed sedentary time, sedentary behaviour patterns (bouts and breaks) and LPA with global long-term (10-year) CVD risk among people living with RA. The extent to which associations are independent of levels of MVPA participation will also be examined. Indeed, interventions which focus on reducing sedentary behaviour and increasing LPA (i.e., promoting the “sit less, move more” message), will likely only be efficacious towards reducing risk of CVD in the instance these behaviours are favourably associated with CVD risk/profile, independently of the possible cardio-protective effects of MVPA engagement. We hypothesise that higher levels of daily sedentary time, and more frequent (and longer) uninterrupted sedentary bouts, will be adversely associated with 10-year CVD risk, after considering daily levels of MVPA participation. In contrast, we expect that the number of sedentary breaks per day, and higher levels of engagement in LPA, will be beneficially associated with 10-year CVD risk, independently of MVPA engagement. Finally, given the lack of research examining the role of sedentary behaviour patterns and LPA for RA outcomes, secondary aims of this study were to explore the role of sedentary time, sedentary behaviour patterns and LPA with individual CVD risk factors and functional disability in RA.
Results
Included (N = 61) versus excluded (N = 36) participants did not differ in terms of age, or any of the targeted outcomes (all p > .05). Chi-square tests confirmed the distribution of males to females was not significantly different among excluded compared to included participants (X (1) = .27, p = .61). No exclusion bias was observed based on ethnicity (X (1) = .36, p = .55).
Descriptive statistics for included participants’ data are reported in Table
1. Accelerometer data revealed participants spent approximately 8 to 9 h/day sedentary (64.84% of accelerometer wear-time), participated in LPA for 4.5 h/day (32.22% of accelerometer wear-time), and broke up their sedentary time approximately 6 times/h. Interpretation of descriptive data indicated 43% of participants had ≥10% risk of CVD at 10-years based on Qrisk-score. Gender differences were not observed for any sedentary behaviour variables or LPA, but were evident for 10-year risk of CVD (
F (1, 53) = 10.62,
p = <.01) and HDL-C (
F (1, 59) = 9.91,
p = <.01, Table
1).
At the time of data collection, the number (%) of participants undertaking different pharmaceutical treatments were as follows; Disease Modifying Anti-Rheumatic Drugs (DMARDS, 53%), anti-TNF therapy (10%), Non-Steroidal Anti-Inflammatory Drugs (NSAIDs, 36%), analgesics (33%), cholesterol lowering medication (23%), medication for hypertension (20%). For each type of medication, one-way ANOVAs demonstrated that overall, targeted outcomes were not significantly different on the basis of current treatment. Exceptions were observed for participants taking cholesterol lowering medication (Q-risk score,
F (1, 53) = 5.88,
p = .02; M ± SD, yes = 23.54 ± 12.89, no = 13.87 ± 12.87), medication for hypertension (ESR,
F (1, 58) = 4.49,
p = .04; M ± SD, yes = 1.23 ± 0.33, no = 0.97 ± 0.39; LDL-C,
F (1, 58) = 4.13,
p = <.05; M ± SD, yes = 0.54 ± 0.11, no = 0.60 ± 0.09; and plasma glucose,
F (1, 59) = 10.41,
p = <.01; M ± SD, yes = 0.73 ± 0.12, no = 0.66 ± 0.04), and anti-TNF therapy (functional disability,
F (1, 58) = 5.85,
p = .02; M ± SD, yes = 1.47 ± 0.14, no = 1.25 ± 0.22). Subsequently, regression analyses which sought to examine associations between sedentary time, sedentary behaviour patterns and LPA with these specific outcomes, were adjusted for current treatment regime as appropriate (see Table
3 legend).
Correlation analyses
The results of bivariate correlations are displayed in Table
2. This analysis revealed that average daily sedentary time (min/day) and the number of sedentary bouts/day ≥20 min were significantly positively associated with 10-year CVD risk. By contrast, daily LPA (min/day) was significantly negatively associated with 10-year CVD risk. However, the number of sedentary breaks/day and average sedentary bout length were not related to a 10-year CVD risk score.
Table 2
Bivariate correlations between accelerometer assessed sedentary behaviour patterns, light physical activity and MVPA with 10-year CVD risk, individual CVD risk factors and functional disability
1 | Age |
2 | Sedentary time (min/day) | .29* | | | | | | | | | | | | | | | | | | | |
3 |
bLPA (min/day) | −.22 | −.78** | | | | | | | | | | | | | | | | | | |
4 |
bMVPA (min/day) | −.22 | −.37** | .35** | | | | | | | | | | | | | | | | | |
5 | Number of Sbreaks (number/day) | −.12 | −.26* | .61** | .03 | | | | | | | | | | | | | | | | |
6 | Number of Sbouts (number/day) | .31* | .81* | −.79** | −.26** | −.67** | | | | | | | | | | | | | | | |
7 | Average time per Sbout (min) | .07 | .34** | −.50** | −.09 | −.56** | .45** | | | | | | | | | | | | | | |
8 | Q-risk | .76** | .37** | −.40** | −.12 | −.21 | .38** | .20 | | | | | | | | | | | | | |
9 |
bCRP (mg/L) | .18 | .05 | −.20 | −.24 | −.20 | .15 | .09 | .28* | | | | | | | | | | | | |
10 |
bESR (mm/h) | .12 | .20 | −.23 | −.21 | −.09 | .21 | −.01 | .17 | .57** | | | | | | | | | | | |
11 | Fibrinogen (g/L) | .25 | .20 | −.27* | −.23 | −.19 | .31* | .03 | .29* | .69** | .71** | | | | | | | | | | |
12 | Total cholesterol (mmol/L) | .18 | .17 | −.10 | −.18 | .04 | .03 | .11 | .09 | .10 | .14 | − .03 | | | | | | | | | |
13 |
bHDL-C (mmol/L) | .05 | −.11 | .27* | .02 | .30* | −.25†
| −.17 | −.33* | −.20 | −.10 | −.24 | .29* | | | | | | | | |
14 |
bLDL-C (mmol/L) | .15 | .22 | −.19 | −.14 | −.13 | .15 | .19 | .19 | .22 | .19 | .06 | .86** | −.09 | | | | | | | |
15 |
bTriglycerides (mmol/L) | .10 | .13 | −.19 | −.17 | −.02 | .08 | .17 | .22 | −.00 | .14 | .11 | .33* | −.24b
| .13 | | | | | | |
16 | Syst-BP (mm HG) | .53** | .19 | −.09 | −.04 | .05 | .17 | .04 | .52** | .16 | .19 | .17 | .11 | .28* | −.11 | .22 | | | | | |
17 | Diast-BP (mm HG) | .37* | .08 | .15 | .02 | .11 | .13 | .01 | .30* | −.05 | −.03 | −.01 | .12 | .23 | −.07 | .22 | .71** | | | | |
18 |
bPlasma insulin (mmol/L) | −.08 | .05 | −.11 | −.21 | −.08 | .13 | −.08 | .16 | .28* | .35* | .37* | −.03 | −.47** | .08 | .37** | .10 | .14 | | | |
19 |
bPlasma glucose (mmol/L) | .02 | .04 | −.04 | .06 | .05 | .01 | .11 | .12 | .06 | .13 | .10 | .07 | −.30* | .12 | .27* | .10 | .19 | .51** | | |
20 |
bHOMA status | −.08 | .06 | −.09 | −.17 | −.02 | .09 | −.01 | .16 | .23 | .30* | .31* | −.04 | −.47** | .05 | .35** | .06 | .12 | .94** | .74** | |
21 | HAQ | .09 | .13 | −.23 | −.27* | −.16 | .17 | .06 | .13 | .18 | .27** | .23 | −.04 | −.03 | .03 | −.11 | .07 | .02 | .26** | − .04 | .18 |
Sedentary time (min/day) was not related to any individual CVD risk factors. However, the number of sedentary bouts/day ≥20 min was significantly positively associated with fibrinogen. In addition, LPA (min/day) was significantly negatively linked to fibrinogen, and both LPA (min/day) and the number of sedentary breaks/day demonstrated significant positive associations with HDL-C. Average sedentary bout length was not related to any individual CVD risk factors. Daily MVPA (min/day) was significantly and negatively linked to plasma levels of CRP, ESR and fibrinogen.
No significant associations were observed between any accelerometer derived variables with functional disability. However, functional disability was positively correlated with plasma insulin and ESR.
Regression analysis
Model 1
Results from the regression analyses are reported in Table
3.
10-year CVD risk; the significant positive associations between both daily sedentary time (min/day) and the number of sedentary bouts/day ≥20 min with10-year CVD risk were sustained after adjusting for accelerometer wear-time (sedentary time, R
2 = .17, number of sedentary bouts ≥20 min, R
2 = .15). The significant negative association observed between LPA (min/day) and 10-year CVD risk was also maintained in wear-time adjusted models (R
2 = .18). Finally, the number of sedentary breaks/day, and the average time per sedentary bout ≥20 min remained unrelated to 10-year CVD risk in wear-time adjusted models.
Table 3
Regression analyses
Q-risk | .41**
| .17 | | .03, .12 | −.49**
| .18 | | −.14, −.04 | −.26 | .05 | | −.54, .04 | .39**
| .15 | | .90, 4.02 | .23 | .04 | | −.29, 2.66 |
Model 2 MVPA
| .44**
| .17 | .00 | .04, .15 | −.48**
| .18 | .00 | −.14, −.04 | --- | --- | | --- --- | .38**
| .15 | .00 | .80, 4.08 | --- | --- | | --- --- |
aCRP | .06 | .00 | | −.00, .00 | −.04 | .00 | | −.00, .00 | −.02 | .00 | | −.01, .01 | .09 | .01 | | −.03, .07 | −.03 | .00 | | −.04, .04 |
aESR | .25 | .05 | | .00, .00 | −.25 | .05 | | −.00, .00 | −.20 | .02 | | −.01, .00 | .30*
| .07 | | .01, .10 | −.02 | .00 | | −.04, .04 |
Model 2 MVPA
| --- | --- | | --- --- | --- | --- | | --- --- | --- | --- | | --- --- | .27 | .07 | .02 | −.00, .10 | --- | --- | | --- --- |
Fibrinogen | .18 | .03 | | −.00, .01 | −.18 | .03 | | −.01, .00 | −.10 | .01 | | −.03, .02 | .26*
| .06 | | .00, .27 | −.05 | .00 | | −.14, .09 |
Model 2 MVPA
| --- | --- | | --- --- | --- | --- | | --- --- | --- | --- | | --- --- | .22 | .09 | .03 | −.03, .25 | --- | --- | | --- --- |
Total Cholesterol | .13 | .02 | | −.00, .01 | −.13 | .01 | | −.01, .00 | .03 | .00 | | −.02, 02 | −.01 | .00 | | −.13, .13 | .12 | .01 | | −.06, .15 |
aHDL-C | −.17 | .03 | | .00, .00 | .18 | .02 | | .00, .00 | .16 | .02 | | −.00, .00 | −.22 | .04 | | −.01, .00 | −.10 | .01 | | −.01, .00 |
aLDL-C | .16 | .02 | | .00, .01 | −.17 | .02 | | −.00, .00 | .01 | .00 | | −.00, .00 | .03 | .00 | | −.01, .01 | .13 | .02 | | −.01, .02 |
aTriglycerides | .12 | .01 | | .00, .00 | −.09 | .01 | | −.00, .00 | .13 | .01 | | −.00, .00 | .02 | .00 | | −.01, .02 | .13 | .02 | | −.01, .02 |
Syst-BP | −.02 | .00 | | −.08, .07 | −.00 | .00 | | −.08, .08 | .12 | .01 | | −.21, .54 | −.04 | .00 | | −2.62, 2.01 | .01 | .00 | | −2.05, 2.25 |
Diast-BP | −.13 | .01 | | −.06, .02 | .16 | .02 | | −.02, .06 | .10 | .01 | | −.13, .27 | −.03 | .00 | | −1.38, 1.11 | .06 | .00 | | −.90, 1.40 |
aPlasma Insulin | .11 | .01 | | −.00, .00 | −.07 | .00 | | −.00, .00 | .02 | .00 | | −.01, .01 | .15 | .02 | | −.01, .05 | −.14 | .02 | | −.04, 01 |
aPlasma glucose | .10 | .01 | | .00, .00 | −.12 | .01 | | .00, .00 | .13 | .01 | | −.00, .00 | .13 | .02 | | −.01, .01 | .18 | .03 | | −.00, .01 |
aHOMA | .12 | .01 | | .00, .00 | −.09 | .01 | | −.00, .00 | .05 | .00 | | −.00, .01 | .12 | .01 | | −.01, .04 | −.05 | .00 | | −.02, .02 |
aHAQ | .09 | .01 | | −.00, .00 | −.11 | .01 | | −.00, .00 | −.01 | .00 | | −.01, .01 | .07 | .00 | | −.02, .04 | −.02 | .00 | | −.03, .02 |
Model 2 - Adjustment for MVPA
The significant positive relationships between daily sedentary time (min/day) and the number of sedentary bouts/day ≥20 min with 10-year CVD risk, were maintained following adjustment for MVPA (i.e., these associations were independent of levels of MVPA participation, model R2
∆ MVPA, sedentary time = 0.00, sedentary bouts ≥20 min = .00). Similarly, the significant negative association between LPA (min/day) and 10-year CVD risk remained after the inclusion of MVPA in regression models (R2
∆ MVPA = .00). The significant positive association between the number of sedentary bouts/day ≥20 min with fibrinogen and ESR were attenuated and no longer significant following adjustment for MVPA (fibrinogen, R2∆ MVPA = .03; ESR, R2∆ MVPA = .02).
No significant associations were observed between daily MVPA with 10-year CVD risk, individual CVD risk factors and functional disability (i.e., where regression models were adjusted for age, gender, accelerometer wear-time, and sedentary behaviour or LPA).
Discussion
This study is the first to examine the associations between objectively assessed daily sedentary time (≤1.5 METS), sedentary behaviour patterns, and LPA engagement (1.6 – 2.9 METS) with long-term (10-year) CVD risk in RA. Results revealed daily sedentary time and the number of sedentary bouts/day ≥ 20 min were positively associated with 10-year CVD risk, with the reverse relationship evidenced for daily LPA participation. Importantly, these significant relationships were observed to be independent of levels of daily MVPA engagement.
Our results suggest that daily sedentary time and LPA engagement may hold implications for 10-year risk of CVD in RA. These findings are aligned with those reported in population based studies, which demonstrate the role of sedentary behaviour and LPA for long-term risk of CVD among adults (e.g., risk of a first atherosclerotic cardiovascular disease event) [
58,
59]. To illustrate the clinical significance of current findings, interpretation of path coefficients indicate that reducing sedentary time by 68 min/day (i.e., the standard deviation), would equate to a 5.5% reduction in 10-year CVD risk, regardless of an individual’s level of MVPA engagement. Similarly, increasing LPA by the same amount would correspond to a 6% reduction in 10-year risk of CVD. Of relevance to these findings is the high correlation between LPA and sedentary time that is also observed in this study. This is indicative of a ‘displacement association’ whereby the action of reducing sedentary time is likely to be synonymous with increasing LPA among people with RA [
60]. As such, any improvements in long-term CVD risk derived from increasing participation in LPA, are likely to be equivalent to those resulting from associated reductions in sedentary time among this patient group. Taken together, our results provide the first evidence to suggest that reducing sedentary time engagement and increasing participation in LPA (i.e., “sit less, move more”), may help to attenuate long-term CVD risk in RA.
The point that current associations between sedentary time and LPA with 10-year risk of CVD, were observed independently of levels of MVPA participation is particularly pertinent to this contention. Indeed, due to the pain symptomatic of RA and concomitant restricted mobility, people with RA represent a patient group for whom engagement MVPA may be challenging. Consequently, it is likely that for these individuals, reducing sedentary time and increasing engagement in LPA, will be perceived as more feasible than realising higher levels of MVPA participation.
We show for the first time that the number of sedentary bouts/day ≥20 min is positively associated with 10-year CVD risk among RA patients. As such, restricting sedentary bouts to less than 20 min via interrupting sedentary time with LPA, may be of benefit towards reducing long-term CVD risk for this patient group. This may also represent a palatable health promotion message for people with RA, e.g., aim to break up your sedentary time every 20 min with LPA. Still, emphasis should also be placed on the
duration of the interruption in sedentary time for this population, not just the act of interrupting itself (i.e., a sedentary break). Indeed, no association was observed between the number of sedentary breaks/day and 10-year risk of CVD in this study. Thus, the simple act of interrupting sedentary time may not be sufficient to induce the necessary physiological mechanisms (e.g., improvements in lipid profile) in order to improve long-term CVD risk for RA patients. Further research is therefore warranted to infer the optimal ‘sedentary break’ duration and associated subsequent intensity of PA engagement likely to induce the required physiological signal to contribute towards a more favourable long-term CVD-risk profile in RA. For example, a recent study revealed that interrupting sedentary time with 2 min periods of light intensity activity was associated with lower systolic blood pressure in overweight and obese adults [
61].
Considering that calculated cardiovascular risk score is a composite score, this raises the question of specifically which
modifiable factors may contribute towards the significant relationships observed herein. Secondary aims of this study were to explore associations between sedentary behaviour patterns and LPA with individual CVD risk factors, thus enabling the contribution of specific factors to be examined. Our results revealed that both daily sedentary time and LPA demonstrated small to moderate non-significant relationships with total and HDL-cholesterol in adjusted analyses (β = −.13 to .18). Whilst not significant, the size of these associations is comparable to significant associations observed in a previous study of RA, in which associations between accelerometer assessed sedentary behaviour and LPA with individual CVD risk factors were examined [
62]. Future research should therefore aim to explore the possibility that variability in cholesterol levels as related to levels of sedentary behaviour and LPA, may represent a physiological pathway through which these behaviours are associated with long-term risk of CVD. This proposition is aligned with the hypothesis that down-regulation of lipoprotein lipase (LPL) activity - an enzyme which catalyses the hydrolysis of circulating triglycerides – is a key physiological mechanism underlying the adverse association between sedentary behaviour and CVD risk. Specifically, LPL activity decreases in response to sedentary behaviour, potentially holding adverse implications for cholesterol profile (e.g., lower HDL-cholesterol) [
63].
With regards to examination of the relationships between sedentary time, sedentary behaviour patterns and LPA with 1) individual CVD risk factors and 2) functional disability, the lack of significant associations reported herein are inconsistent with findings from extant research [
22‐
24,
36‐
40,
43,
46,
47,
64]. Only the number of sedentary bouts/day ≥ 20 min was significantly related to secondary outcomes (i.e., positive associations with fibrinogen and ESR). Previous studies conducted among non-RA populations, have revealed sedentary time, sedentary behaviour patterns, and LPA to be linked to serological markers of CVD and cardio-metabolic health (e.g., CRP, HDL-cholesterol, blood pressure and plasma glucose, HOMA status), as well as physical function in both healthy adults and clinical cohorts. Our findings also contradict results reported by Khoja et al., (2016) - the only existing study to examine associations between objectively assessed sedentary behaviour and LPA with markers of CVD and physical function in RA specifically [
62]. In the case of Khoja and colleagues (2016), incongruent findings may represent inconsistencies with regards to the way sedentary behaviour and LPA were defined. In this study, we applied the widely accepted ≤1.5 MET definition of sedentary behaviour, as advocated by the sedentary behaviour research network [
35], and considered LPA to represent activities requiring 1.6 – 2.9 METs [
65,
66]. In contrast, Khoja et al., (2016) defined sedentary behaviour and LPA as activities requiring <1 MET, and 1 – 2.9 METS, respectively. As a consequence, this may have resulted in exclusion of common sedentary behaviours requiring energy expenditure of 1 – 1.5 METS (e.g., sitting and reading a book or typing [
65,
67]), and subsequent underestimation of sedentary time engagement/overestimation of LPA.
Still, current results diverge from those observed among such studies of non-RA populations, in which sedentary behaviour (≤1.5 METs) and LPA (1.6–2.9 METS) are reported to be related to CVD risk profile and physical function [
22‐
24,
36‐
40,
43,
46,
47,
64]. The absence of significant relationships in the current study, may therefore be due in part to the disproportionately higher levels of inflammation observed in RA, relative to other populations. That is, the effects of sedentary behaviour and LPA on specific serological markers of CVD and physical function, may be comparatively small when considering the chronic and elevated-inflammatory disease-state characteristic of RA. Thus, in order to reduce systemic inflammation, and improve physical function in RA, the dose and intensity of PA engagement may need to be higher than reported for non-RA populations (i.e., MVPA vs LPA, respectively).
Limitations to the current study include a restricted sample size following data reduction procedures, and a cross-sectional study design. As outlined, longitudinal and experimental studies among larger samples are therefore required to confirm the relationships reported herein. Still, this is the first study to employ accelerometers to explore the implications of sedentary behaviour patterning and LPA for cardiovascular health among RA patients. We also acknowledge that conducting repeated regression analyses to examine the hypothesised associations might have inflated the chance of Type 1 error. However, this is the first study to examine relationships between sedentary behaviour patterns and LPA across a broad range of CVD related outcomes in RA. Therefore, we believe that it is of interest to analyse and report results pertaining to all relevant CVD variables examined within the PARA study, even in the instance that null or significant relationships were observed. Finally, the use of the GT3X in this study means the definition of sedentary behaviour employed considers only energy expenditure (i.e., ≤1.5 METs). That is, we were not able to examine whether behaviours characterised by ≤1.5 METS (<100 counts per minute), occurred whilst sitting/lying vs. standing. Still, studies to date examining the implications of sedentary behaviour for health across diverse populations have largely employed accelerometers to measure sedentary time [
52]. Thus, the results presented herein facilitate comparisons with other relevant literature. Nevertheless, future studies should seek to employ devices that enable assessment of posture (e.g., the activPAL) alongside traditional accelerometry based approaches used to measure sedentary time, in order to more accurately determine the implications of sedentary behaviour for health outcomes in RA.
Acknowledgements
The authors would like to acknowledge all of the participants who gave their time to participate in the study. In addition, we would like to thank the staff at the Clinical Research Unit and Russells Hall Hospital who provided assistance with data collection.